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6330514A18Rik (BC099679) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209261
6330514A18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 6330514A18 gene (cDNA clone MGC:106082 IMAGE:6489647)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $554.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 6330514A18Rik
Synonyms 6330514A18Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209261 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCCGGTCCCGGCGGCGGCGGAGCCGCCAGCTTGTGGCCACCTTCCTGCGCGACCCAGGCTCGG
GGCGCGTGTACCGGCGCGGGAAGCTGATCGGCAAGGGAGCCTTCAGCCGCTGCTACAAGCTCACGGACAT
GTCCACCAGCGCTGTGTTCGCTCTTAAGGTGGTGCCAAGGGGCGGAGCGGGGCGGCTGCGCCTGCGCGGG
AAGGTGGAGCGTGAGATCGCCCTGCACAGCCGCCTGCGCCACCGCAACATCGTGGCCTTCCACGCACACT
TTGCTGACCGCGATCACGTGTACATGGTGCTGGAGTATTGTAGCCGCCAGTCCTTGGCTCATGTGCTGAA
AGTGCGGCGGACTCTGACAGAGCCTGAAGTGCGCTATTACCTTCGGGGCCTGGTCAGCGGCCTGCGCTAC
TTGCACCAACAACGCATCGTGCACAGGGACCTGAAACCCAGTAATTTCTTCCTTAACAAGAACATGGAGG
TTAAGATAGGTGACCTGGGGCTGGCTGCCCGTGTGGGGCCTGCTGGTCGCTGCCACAGGGTGCTCTGCGG
GACTCCAAACTTCCAGGCACCTGAAGTGGTGTCCCGAAATGGACACTCGTGCAAATCTGACATCTGGGCT
CTGGGATGCATCATGTACACGGTGCTGACAGGCACGCCACCCTTTGCCGCGGCACCCTTGTCAGAGATGT
ACCAAAACATCCGTGACGGCCACTACCTCGAGCCTACTCACCTGTCGCCCAGCGCCCGCAGCCTCATTGC
ACGGCTCCTGGCACCGGACCCCGCCGAGCGGCCCAGCCTGGACCACTTACTGCAAGATGACTTCTTCAGT
CAGGGCTTCACCCCGGAGCGGCTGCCTCCACATTCCTGCCACAGCCCACCTGTGTTTGCCTTTCCCCCAC
CTCTGGGCAGGTTGTTCCGGAAGGTGGGCCAGCTCCTGCTGACCCAGTGCCGGCCACCTTGTCCCTTTAC
CTCAAAAGAGGCCTCAGGCCCTGGAGAAGAGGGCACAGAACCAGACCACATGGAAGCCGGCAACGAGGAA
AGGGATCCTCTCTGCACCGAGGGCCGAATCCACCTCCTCACCCTTGGGACCCCGCGGACTGGCCTGGCGG
GCCCAAAAGGGAGCTTGGCTCTGCAGCTGGAAGTGGCAACCAGAAAACTGTTCCTCTGCTTGGACGCAGG
ACCTATGGCTGGACAGGACCCCCCAGGGGAGCAGCGGCCTGTTCTCTGGGCTCCTAAGTGGGTGGATTAC
TCCCTCAAGTATGGCTTCGGCTACCAGCTGTCGGATGGGGGCAGTGGGGTGCTGTTTCGGGATGGCTCCC
ACATGGCCCTGCGCCCCCAAGGCGGCCATGTCTCCTACCAACCTGACCAAGGGACTCTCTGGACCTTCAC
CCTGAGGGACGTCCCCAGCCCACTTCGTGCCAAGCTGGCTGTCCTGCGGCTCTTTGCCTGCTATATGCAG
CGGCGTCTGCGGGAGGAGGGGACTGTGCCCATGCCTGCCACACCTGCCTCTCCTGACATCTCTCTGCTGA
GCTTCATTGCTGACTCCCAGGCTATGGTCATGCTGTTTAGCAATGGGACAGTGCAGGTCAGCCTCAAAAC
CTCCCAAACCCAGCTGGTGCTAAGTGGGGAGGACGAGGACCTCCTGCTCACCCTCCAGGAGCCAGGGGGA
CCCGCTGTGGGCGTCTCCTACACACTCGATGTCCTGCGGAGCCATGGCTTCACCCTTGCAGTCCACCATC
ACCTGCGCCATGGCCTGCATCTGCTACAGAGTGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209261 protein sequence
Red=Cloning site Green=Tags(s)

MEPRSRRRRSRQLVATFLRDPGSGRVYRRGKLIGKGAFSRCYKLTDMSTSAVFALKVVPRGGAGRLRLRG
KVEREIALHSRLRHRNIVAFHAHFADRDHVYMVLEYCSRQSLAHVLKVRRTLTEPEVRYYLRGLVSGLRY
LHQQRIVHRDLKPSNFFLNKNMEVKIGDLGLAARVGPAGRCHRVLCGTPNFQAPEVVSRNGHSCKSDIWA
LGCIMYTVLTGTPPFAAAPLSEMYQNIRDGHYLEPTHLSPSARSLIARLLAPDPAERPSLDHLLQDDFFS
QGFTPERLPPHSCHSPPVFAFPPPLGRLFRKVGQLLLTQCRPPCPFTSKEASGPGEEGTEPDHMEAGNEE
RDPLCTEGRIHLLTLGTPRTGLAGPKGSLALQLEVATRKLFLCLDAGPMAGQDPPGEQRPVLWAPKWVDY
SLKYGFGYQLSDGGSGVLFRDGSHMALRPQGGHVSYQPDQGTLWTFTLRDVPSPLRAKLAVLRLFACYMQ
RRLREEGTVPMPATPASPDISLLSFIADSQAMVMLFSNGTVQVSLKTSQTQLVLSGEDEDLLLTLQEPGG
PAVGVSYTLDVLRSHGFTLAVHHHLRHGLHLLQSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC099679
ORF Size 1785 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC099679, AAH99679
RefSeq Size 2089 bp
RefSeq ORF 1787 bp
Locus ID 216166
Cytogenetics 10 C1
MW 66.1 kDa
Summary Inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation.UniProtKB/Swiss-Prot Function
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"BC099679" in other vectors (4)
SKU Description Size Price
MC218577 6330514A18Rik (untagged) - Mouse RIKEN cDNA 6330514A18 gene (cDNA clone MGC:106082 IMAGE:6489647), (10ug) 10 ug
$610.00
MG209261 6330514A18Rik (tGFP-tagged) - Mouse RIKEN cDNA 6330514A18 gene (cDNA clone MGC:106082 IMAGE:6489647) 10 ug
$754.00
MR209261L3 Lenti ORF clone of 6330514A18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 6330514A18 gene (cDNA clone MGC:106082 IMAGE:6489647) 10 ug
$854.00
MR209261L4 Lenti ORF clone of 6330514A18Rik (mGFP-tagged) - Mouse RIKEN cDNA 6330514A18 gene (cDNA clone MGC:106082 IMAGE:6489647) 10 ug
$854.00

Plasmid Map for MR209261

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209261
Circular map for MR209261

file-alt Citations

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