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5430413K10Rik (BC080814) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209220
5430413K10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5430413K10 gene (cDNA clone MGC:91146 IMAGE:6391119)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $550.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 5430413K10Rik
Synonyms 4833413D08Rik; AI413838
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209220 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGTTCTCCAGGCCTTGGCCATCATGTTGAGCATCCAAGCGGGAACACTTGACCTGGTGGAAACAC
CCCCAGTGGTGGGCAATCTTCCTGTTGCCATGCCTGTTCCACTCAATCTCCCTGTAGGAGGCCTAAGTCC
TCCAGTCCTCAAAGGACCAGTCAACCATCAAATGTTACCTCCTAAAAGGCCTGTACCTCCACCCAAAGGT
GGCAAGTGTGCACCTGCAGCCAGATACTTCCTCTCCAGTGACAAACTCCATGACTACCTCATGAGCACAC
TGCCCCCACAGATTGAGGACATGGTGAAGTGTGATGAAGTTAACCTGGAAGGCATGCTTGCGGATGTGTT
GAACACTGTGGAGAGCTCTGACCTGCTCTCCCTCTTAGATGGCATTTCCCTCCTAAAAGGAGGAGAAGGG
GGAGGCCTTGGCATTGGTGGACTCCTAGGCAATGAAGGCAATGGGGATTCCTCAAAGCCCTCATCAGGCT
CCAAGGCCACTGGTGGTCTTGGCCAGCTAATCCCAGGGGGCATCCCAGGCACGGAGGCCCTGGGCGGCCT
ACTAAACCTCGGTGGAGACAAGAGCTCTGGAAAAGGGCTGCTGAATGGGGATGGGCTCTCCAAAATCAAG
AAGCCTCTTGAGGATGCAGTTGAAAATGTGAGCGGTATAAAAGATGCCATTCAGGAGAAAGTGAATGAAG
TGGTCCCAGATGGCGTCAAGGAGCCACTTAACGATGTGCTCAAGATGGATATCAAAGACACTCTGCTCGA
ACTGAAGGTCGGACAAGTAACTTTGGACGACATGGAGATAAACATGGAAGCTAATGGCATGCAAGTTCTC
TCCATGCTTACTGCTACCATAGATGGAAAAGGCGTGCTTGGACCAGTCATCAGCCTACTGCAGTTTGAAG
CTAAGATGGATGTAATGACGACAATTGCTGTTGCCTCCAACAACACCCAGTGTGTCAACCTTGATGCCCA
AGACACCCACATGCATGTCAAGGAAATGAAAATTCAGTTAGTGGAGACAGTCACAGGAAAAGTGCCTCTG
CCTGTGCCTCTGCCCTTGGATCAGATCATCCCAGCAATAGTGACAGCAAAAATAAATGAAAATCTGGAAA
AATCCAACTCCTGTGCCATTGTCCTCAATGACTTCAACAACTGCAAGAACAATACCGGCTTATTCAGTTA
CCAAGTTAATACTGCCAGGATCTCTCCAAAAGGACTTGTCATCCTCTACTGTGCTAAAGCCAATATAGGC
AACAAAACAGTACCTGTGCCTGGAGGTCGGCTGCCTCCAGATCCCAAAAATGCAAGCATTGCGGTAACCA
TTTCCTCCACAACACTGAAGACACTTGTAAAAGAAGTGGCCAAGAATAGCTCTGTGCAGATGGATGGCCT
GGAAGCACAAATCACCCACATAGCGTTTGCCTCCCAGGAAAACAATACGCTCAGAGTCGTGTATAAGGTT
GACATAACAAAGAACGGAGAGCACTTTGCCACTGGGGAAACGAAATTATTCATCTCCCATGGCAGCAAGA
TTTCAAACTCCACACTGATACCAGATGTCAAGCTCATAAGGTCTGAGCACAGTGTGGTGCCTCCTGAGGC
TAAGGAAGAAGTAGAAGGCATTTTGTCTGAAGTAGGAAAGGTGGCCTGGTCCAACTTCAATGAAACGTAT
AAAAAAATGAACATTCCTGTAGGTGTGTCTTCACATACTTTAAAGAACTCCGATGTCAAATTGATGAAAT
CAATTGACCTTCAGGCAGCAAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209220 protein sequence
Red=Cloning site Green=Tags(s)

MWVLQALAIMLSIQAGTLDLVETPPVVGNLPVAMPVPLNLPVGGLSPPVLKGPVNHQMLPPKRPVPPPKG
GKCAPAARYFLSSDKLHDYLMSTLPPQIEDMVKCDEVNLEGMLADVLNTVESSDLLSLLDGISLLKGGEG
GGLGIGGLLGNEGNGDSSKPSSGSKATGGLGQLIPGGIPGTEALGGLLNLGGDKSSGKGLLNGDGLSKIK
KPLEDAVENVSGIKDAIQEKVNEVVPDGVKEPLNDVLKMDIKDTLLELKVGQVTLDDMEINMEANGMQVL
SMLTATIDGKGVLGPVISLLQFEAKMDVMTTIAVASNNTQCVNLDAQDTHMHVKEMKIQLVETVTGKVPL
PVPLPLDQIIPAIVTAKINENLEKSNSCAIVLNDFNNCKNNTGLFSYQVNTARISPKGLVILYCAKANIG
NKTVPVPGGRLPPDPKNASIAVTISSTTLKTLVKEVAKNSSVQMDGLEAQITHIAFASQENNTLRVVYKV
DITKNGEHFATGETKLFISHGSKISNSTLIPDVKLIRSEHSVVPPEAKEEVEGILSEVGKVAWSNFNETY
KKMNIPVGVSSHTLKNSDVKLMKSIDLQAAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC080814
ORF Size 1773 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC080814, AAH80814
RefSeq Size 2070 bp
RefSeq ORF 1775 bp
Locus ID 71425
Cytogenetics 2 H1
MW 62.4 kDa
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"BC080814" in other vectors (4)
SKU Description Size Price
MC217875 5430413K10Rik (untagged) - Mouse RIKEN cDNA 5430413K10 gene (cDNA clone MGC:91146 IMAGE:6391119), (10ug) 10 ug
$606.00
MG209220 5430413K10Rik (tGFP-tagged) - Mouse RIKEN cDNA 5430413K10 gene (cDNA clone MGC:91146 IMAGE:6391119) 10 ug
$750.00
MR209220L3 Lenti ORF clone of 5430413K10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5430413K10 gene (cDNA clone MGC:91146 IMAGE:6391119) 10 ug
$850.00
MR209220L4 Lenti ORF clone of 5430413K10Rik (mGFP-tagged) - Mouse RIKEN cDNA 5430413K10 gene (cDNA clone MGC:91146 IMAGE:6391119) 10 ug
$850.00

Plasmid Map for MR209220

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209220
Circular map for MR209220

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