Rec8 (NM_020002) Mouse Tagged ORF Clone
$826.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Rec8 |
| Synonyms | mrec; Rec8L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_020002 |
| ORF Size | 1773 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_020002.3, NP_064386.2 |
| RefSeq Size | 2055 bp |
| RefSeq ORF | 1776 bp |
| Locus ID | 56739 |
| UniProt ID | Q8C5S7 |
| Cytogenetics | 14 28.19 cM |
| MW | 67.4 kDa |
| Summary | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_020002" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC219409 | Rec8 (untagged) - Mouse REC8 homolog (yeast) (Rec8), (10ug) | 10 ug |
$881.00
|
|
| MG209218 | Rec8 (tGFP-tagged) - Mouse REC8-like 1 (yeast) (Rec8L1) | 10 ug |
$1,026.00
|
|
| MR209218L3 | Lenti ORF clone of Rec8 (Myc-DDK-tagged) - Mouse REC8 homolog (yeast) (Rec8) | 10 ug |
$1,126.00
|
|
| MR209218L4 | Lenti ORF clone of Rec8 (mGFP-tagged) - Mouse REC8 homolog (yeast) (Rec8) | 10 ug |
$1,126.00
|
|
Citations
Ectopic expression of meiotic cohesin RAD21L promotes adjacency of homologous chromosomes in somatic cells
Rong, Miyauchi, Lee
J. Reprod. Dev. (2017)
Rong, Miyauchi, Lee
J. Reprod. Dev. (2017)
AI Summary: OriGene products contributed to this study by providing expression plasmids for RAD21-DDK, REC8-DDK, SMC3-DDK, and SMC1β-DDK, as well as the pCMV6-AC-mGFP vector, which were used to investigate the effects of ectopic expression of meiotic cohesin subunits on homologous chromosome adjacency in somatic cells.
Showing 1-1 of 1 papers.
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