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Cdk5rap1 (NM_025876) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209175
Cdk5rap1 (Myc-DDK-tagged) - Mouse CDK5 regulatory subunit associated protein 1 (Cdk5rap1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $548.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdk5rap1
Synonyms 2310066P17Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209175 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCCTTTACGGTGTGTCCTCCAAGTACAGAGGTTGTCAGCACCATTCACCTCCATGTGCTGGGTGT
TGCTTAGGACCTGCAGAGCACAAAGCAGTGTGTCCAGCACTCCTTGTCCCAGTCCAGAGGCGAAGAGCTC
AGAAGCTCAGAAGGACTTCAGCTCCAGGCTAGCCACTGGACCGACTTTTCAGCATTTTTTAAGAAGTGCT
TCAGTTCCTCAAGAGAAACCATCTTCTCCAGAGGTGGAGGACCCACCTCCCTATCTCTCGGGGGATGAAC
TTCTAGGAAGGCAGAGAAAAGTCTACCTCGAGACCTATGGCTGTCAGATGAACGTGAACGACACAGAGAT
AGCCTGGTCCATCTTACAGAAGAGTGGCTACCTTCGGACCAGCAACCTCCAAGAGGCTGATGTGATCCTT
CTTGTCACGTGTTCTATCAGGGAGAAGGCCGAGCAGACCATCTGGAACCGCTTACATCAGCTCAAAGTCC
TGAAGACAAAGCGGCCACGCTCTCGAGTACCTCTGAGGATTGGGATTCTAGGCTGCATGGCTGAGAGGCT
GAAGGGAGAGATCCTCAACAGGGAGAAGATGGTAGATCTCTTGGCTGGTCCAGACGCCTACCGAGACCTT
CCCCGGCTGCTGGCTGTGGTGGAGTCAGGTCAGCAAGCAGCAAATGTGCTTCTCTCTCTGGATGAGACCT
ACGCAGATATCATGCCAGTCCAGACGAGCCCCAGTGCCACTTCTGCCTTTGTATCCATTATGCGGGGCTG
CGACAACATGTGCAGTTACTGTATCGTGCCGTTCACGCGTGGCCGGGAGAGGAGCCGGCCAGTTGCCTCC
ATTCTCGATGAAGTGAGGAAGCTGTCCGAGCAGGGCCTAAAAGAAGTGACACTTCTTGGTCAGAATGTTA
ATAGTTTTCGGGACAATTCGGAAGTCCAGTTCAATAATGCAGGGTCTGCTAACCTCAGCCGTGGCTTTAC
CACCAACTATAAACCCAAACAAGGAGGGCTTCGTTTTTCTCACCTTCTGGATCAGGTTTCAAGAATCGAT
CCTGAAATGAGGATCCGATTCACCTCTCCTCACCCCAAGGATTTTCCTGATGAGGTTCTTCAGCTGATTC
GTGAGAGACACAACATCTGTAAGCAGATCCACCTGCCAGCCCAGAGTGGAAGCAGCCGTGTACTGGATGC
CATGCGGAGAGGATATTCAAGAGAAGCATATGTGGCATTAGTTCATCATGTCAGAGAGACTATCCCAGGT
GTGAGCCTTAGCAGCGACTTCATCACTGGCTTTTGTGGAGAGACAGAGGATGATCACCGGCAGACAGTGT
CTTTACTTAGAGAAGTGCAGTATAATACCGGTTTTCTCTTTGCATACAGCATGAGGCAAAAGACACGAGC
ATATCACAGGCTGAAGGATGATGTTCCAGAAGAAGTAAAATTAAGGCGTTTGGAGGAACTCATTACTGTC
TTCAGAGAAGAAGCTTCAAAAGCCAATAAGACCTCAGTGGGTTGTAGCCAGCTGGTACTGGTTGAAGGGT
TCAGCAAACGTTCTACCACAGACCTTTGTGGCCGGAATGATGCAAACCTTAAGGTGATTTTCCCTGACGC
AGAGGTGGAGGATATCACTAACCCTGGGCTCAAGGTCAGAGCTCAACCTGGGGACTATGTGCTGGTGAAG
ATCACCTCTGCCAGCTCTCAGACCCTGAAAGGACACATTCTCTGCAGGACCACTATGAAAGACTCATTGA
CGTATTGCACGACC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209175 protein sequence
Red=Cloning site Green=Tags(s)

MHPLRCVLQVQRLSAPFTSMCWVLLRTCRAQSSVSSTPCPSPEAKSSEAQKDFSSRLATGPTFQHFLRSA
SVPQEKPSSPEVEDPPPYLSGDELLGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVIL
LVTCSIREKAEQTIWNRLHQLKVLKTKRPRSRVPLRIGILGCMAERLKGEILNREKMVDLLAGPDAYRDL
PRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPVAS
ILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQFNNAGSANLSRGFTTNYKPKQGGLRFSHLLDQVSRID
PEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQSGSSRVLDAMRRGYSREAYVALVHHVRETIPG
VSLSSDFITGFCGETEDDHRQTVSLLREVQYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITV
FREEASKANKTSVGCSQLVLVEGFSKRSTTDLCGRNDANLKVIFPDAEVEDITNPGLKVRAQPGDYVLVK
ITSASSQTLKGHILCRTTMKDSLTYCTT

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_025876
ORF Size 1764 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025876.2, NP_080152.1
RefSeq Size 1970 bp
RefSeq ORF 1767 bp
Locus ID 66971
UniProt ID Q8BTW8
Cytogenetics 2 H1
MW 66.1 kDa
Summary Probable regulator of CDK5 activity. May inhibit CDK5 function via its interaction with CDK5R1 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025876" in other vectors (4)
SKU Description Size Price
MC205818 Cdk5rap1 (untagged) - Mouse CDK5 regulatory subunit associated protein 1 (Cdk5rap1), (10ug) 10 ug
$548.00
MG209175 Cdk5rap1 (tGFP-tagged) - Mouse CDK5 regulatory subunit associated protein 1 (Cdk5rap1) 10 ug
$748.00
MR209175L3 Lenti ORF clone of Cdk5rap1 (Myc-DDK-tagged) - Mouse CDK5 regulatory subunit associated protein 1 (Cdk5rap1) 10 ug
$848.00
MR209175L4 Lenti ORF clone of Cdk5rap1 (mGFP-tagged) - Mouse CDK5 regulatory subunit associated protein 1 (Cdk5rap1) 10 ug
$848.00

Plasmid Map for MR209175

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR209175
Circular map for MR209175

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