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Mars2 (NM_175439) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209149
Mars2 (Myc-DDK-tagged) - Mouse methionine-tRNA synthetase 2 (mitochondrial) (Mars2), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $545.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mars2
Synonyms C730026E21Rik; MetRS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209149 representing NM_175439
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGGCAGTGTGCTCGCTGGGTGTTGACGCGCACCCGGTTCGGCCGCGGCTGTCGCCGCTACGGTT
CGTGCTCGCCGAGCGCTAGTGGCGACGCCGGGGAAGCGCGGGCCTACTTCACCACACCTATCTTCTACGT
GAACGCCGCGCCGCACATCGGGCACCTGTACTCGGCGCTACTGGCCGACGCCCTGTGCCGCCACCGTCGT
CTCCGAGTTCCCGGCTCTGCGTCCACGCGGTTTTCCACCGGCACGGATGAGCACGGCCTGAAGATCCAGC
AGGCGGCAGCCACCGCCGGCTTGGCCCCGATCGAGCTGTGCGATCGAGTGTCTGCCCAGTTTTTGCAGCT
TTTCCGGGAGGCTGACATCTCTTCCACGGACTTCATCCGCACCACGGAGGCTCGGCACCGAGTAGCGGTG
CAGCATTTCTGGGGTGTGCTGGAGGCCCGGGGTCTGCTTTACAAAGGGATCTATGAAGGGTGGTATTGTG
CCTCGGATGAGTGCTTCTTGCCGGAGGCCAAGGTTACTCGGCAGGTGGGTCCGTCCGGGGATCCCTGTCC
CGTGTCTCTCGAGAGCGGACATCCTGTCTCCTGGACGAAGGAAGAAAATTACATTTTCAAGCTTTCTCAG
TTTCGAGAACCACTCCAGCGGTGGCTTGGAAACAATCCTCAGGCAATCACACCGGAACCGTTCCACCAGG
CAGTTCTTCAGTGGCTGGAGGAGGAGCTGCCCGACTTGTCCGTTTCTCGAAGGAGTAGCCATCTGCACTG
GGGCATACCAGTTCCCGGGGACGACTCGCAGACCATCTACGTGTGGCTGGATGCCCTGGTCAACTACCTC
ACTGTAGTTGGCTACCCAGATGCAGATTTCAAGTCTTGGTGGCCAGCCACCTCTCATATCATAGGTAAGG
ACATTCTCAAATTTCATGCTATTTATTGGCCTGCCCTTCTCCTAGGGGCAGGATTGAGGCCACCACATCG
CATCTATGTCCACTCGCACTGGACGGTGAGTGGCCAAAAAATGTCCAAAAGCTTGGGCAACGTGGTGGAT
CCCAGGACTTGCCTTGATCGCTATACTGTGGATGGCTTTCGCTACTTTCTCCTTCGGCAGGGCGTTCCCA
ATTGGGACTGCGATTACTATGATGAAAAGGTGGTTAAATTGCTGGACTCCGAGCTGGCAGATGCCTTAGG
AGGGCTTTTAAACCGGTGCACTGCCTACAGAATAAATCCTTCTGGAACCTACCCATCTTTCTGTGCTGCC
TGTTTCCCCAGTGAGCCAGGGTTAACGGGGCCGTCAGTTCGTGTGCAGGCAGAAGACTATGCGCTTGTAA
CTGCAGTGGCCACTTTACCCAAACTGGTAGCGGGCTATTATAATGACTTTCAGATCTATAAGGCTCTGGA
GGCGGTATCCAGCTGTGTTCGGCAAACTAATGGTTTCGTCCAAAGGCACGCACCATGGAAATTGAACTGG
GAGAGCCCTGAGGATGCTCCCTGGCTGGGTACCGTGCTTCACGTAGCCTTGGAGTGCTTGCGAGTGTTTG
GAACTTTGCTTCAGCCTGTTACTCCAAACTTAGCAGATAAACTGCTGTCAAGACTGGGAGTCTCTACCAC
AGAGAGAGGCCTTGGAGAGCTCTACTTCCTACCTCGATTCTATGGACACCCATGCCCCTTTGAGGGGAGA
AAGTTGGGACCTGACACTGGGCTTTTGTTTCCTAGACTGGACCAATCTAGGACTCGGCTAGTGAAAGCCC
ACAGGACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209149 representing NM_175439
Red=Cloning site Green=Tags(s)

MLRQCARWVLTRTRFGRGCRRYGSCSPSASGDAGEARAYFTTPIFYVNAAPHIGHLYSALLADALCRHRR
LRVPGSASTRFSTGTDEHGLKIQQAAATAGLAPIELCDRVSAQFLQLFREADISSTDFIRTTEARHRVAV
QHFWGVLEARGLLYKGIYEGWYCASDECFLPEAKVTRQVGPSGDPCPVSLESGHPVSWTKEENYIFKLSQ
FREPLQRWLGNNPQAITPEPFHQAVLQWLEEELPDLSVSRRSSHLHWGIPVPGDDSQTIYVWLDALVNYL
TVVGYPDADFKSWWPATSHIIGKDILKFHAIYWPALLLGAGLRPPHRIYVHSHWTVSGQKMSKSLGNVVD
PRTCLDRYTVDGFRYFLLRQGVPNWDCDYYDEKVVKLLDSELADALGGLLNRCTAYRINPSGTYPSFCAA
CFPSEPGLTGPSVRVQAEDYALVTAVATLPKLVAGYYNDFQIYKALEAVSSCVRQTNGFVQRHAPWKLNW
ESPEDAPWLGTVLHVALECLRVFGTLLQPVTPNLADKLLSRLGVSTTERGLGELYFLPRFYGHPCPFEGR
KLGPDTGLLFPRLDQSRTRLVKAHRT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175439
ORF Size 1758 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175439.3, NP_780648.1
RefSeq Size 2882 bp
RefSeq ORF 1761 bp
Locus ID 212679
UniProt ID Q499X9
Cytogenetics 1 C1.2
MW 65.8 kDa
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Documents
Resources
"NM_175439" in other vectors (4)
SKU Description Size Price
MC219335 Mars2 (untagged) - Mouse methionine-tRNA synthetase 2 (mitochondrial) (Mars2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$601.00
MG209149 Mars2 (tGFP-tagged) - Mouse methionine-tRNA synthetase 2 (mitochondrial) (Mars2) 10 ug
$745.00
MR209149L3 Lenti ORF clone of Mars2 (Myc-DDK-tagged) - Mouse methionine-tRNA synthetase 2 (mitochondrial) (Mars2), nuclear gene encoding mitochondrial protein 10 ug
$845.00
MR209149L4 Lenti ORF clone of Mars2 (mGFP-tagged) - Mouse methionine-tRNA synthetase 2 (mitochondrial) (Mars2), nuclear gene encoding mitochondrial protein 10 ug
$845.00

Plasmid Map for MR209149

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209149
Circular map for MR209149

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