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Faah (NM_010173) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209084
Faah (Myc-DDK-tagged) - Mouse fatty acid amide hydrolase (Faah)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $540.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Faah
Synonyms AW412498
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209084 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCTGAGCGAAGTGTGGACCGCGCTGTCTGGACTCTCCGGGGTTTGCCTAGCCTGCAGCTTGCTGT
CGGCGGCGGTGGTCCTGCGATGGACCAGGAGCCAGACCGCCCGGGGCGCGGTGACCAGGGCGCGGCAGAA
GCAGCGAGCCGGCCTGGAGACCATGGACAAGGCGGTGCAGCGCTTCCGGCTGCAGAATCCTGACCTGGAT
TCACAGGCCTTGCTGGCTCTGCCCCTGCTCCAACTGGTACAGAAGTTACAGAGTGGGGAACTGTCCCCAG
AAGCTGTGCTCTTTACCTACCTGGGAAAGGCCTGGGAAGTGAACAAAGGGACCAACTGTGTGACCTCCTA
TCTGACTGACTGTGAGACTCAGCTGTCCCAGGCCCCACGGCAGGGCCTGCTCTATGGCGTCCCCGTGAGC
CTCAAGGAATGCTTCAGCTACAAGGGCCATGCTTCCACACTGGGCTTAAGTTTGAACGAGGGTGTGACAT
CGGAGAGTGACTGTGTGGTGGTGCAGGTACTGAAGCTGCAGGGAGCTGTGCCCTTTGTGCACACCAACGT
CCCCCAGTCCATGCTAAGCTATGACTGCAGTAACCCCCTCTTTGGCCAGACCATGAACCCGTGGAAGCCC
TCCAAGAGTCCAGGAGGTTCCTCAGGGGGTGAGGGGGCTCTCATTGGATCTGGAGGCTCCCCTCTGGGTT
TAGGCACTGACATCGGCGGCAGCATCCGGTTCCCTTCTGCCTTCTGTGGCATCTGTGGCCTCAAGCCTAC
TGGGAACCGCCTCAGCAAGAGTGGCCTGAAGAGCTGTGTTTATGGACAGACAGCAGTGCAGCTTTCTGTT
GGCCCCATGGCACGGGATGTGGATAGCCTGGCATTGTGCATGAAAGCCCTACTTTGTGAGGATTTGTTCC
GCTTGGACTCCACCATCCCCCCCTTGCCCTTCAGGGAGGAGATCTACAGAAGTTCTCGACCCCTTCGTGT
GGGATACTATGAAACTGACAACTACACCATGCCCACTCCAGCCATGAGGAGGGCTGTGATGGAGACCAAG
CAGAGTCTCGAGGCTGCTGGCCACACGCTGGTCCCCTTCTTACCAAACAACATACCTTATGCCCTGGAGG
TCCTGTCGGCAGGTGGGCTGTTCAGTGATGGTGGCTGCTCTTTTCTCCAAAACTTCAAAGGCGACTTTGT
GGATCCCTGCTTGGGGGACCTGGTCTTAGTGCTGAAGCTGCCCAGGTGGTTTAAAAAACTGCTGAGCTTC
CTGCTGAAGCCTCTGTTTCCTCGGCTGGCAGCCTTTCTCAACAGTATGTGTCCTCGGTCAGCCGAAAAGC
TGTGGGAACTGCAGCATGAGATTGAGATGTATCGCCAGTCCGTCATTGCCCAGTGGAAGGCAATGAACTT
GGACGTGGTGCTAACCCCCATGCTGGGTCCTGCTCTGGATTTGAACACACCGGGCAGAGCCACAGGGGCT
ATCAGCTACACTGTTCTCTATAACTGCCTGGACTTCCCTGCGGGGGTGGTGCCTGTCACCACTGTGACCG
CTGAGGACGATGCCCAGATGGAACACTACAAAGGCTACTTTGGGGATATGTGGGACAACATTCTGAAGAA
GGGCATGAAAAAGGGTATAGGCCTGCCTGTGGCTGTGCAGTGCGTGGCTCTGCCCTGGCAGGAAGAGCTG
TGTCTGCGGTTCATGCGGGAGGTGGAACGGCTGATGACCCCTGAAAAGCGGCCATCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209084 protein sequence
Red=Cloning site Green=Tags(s)

MVLSEVWTALSGLSGVCLACSLLSAAVVLRWTRSQTARGAVTRARQKQRAGLETMDKAVQRFRLQNPDLD
SQALLALPLLQLVQKLQSGELSPEAVLFTYLGKAWEVNKGTNCVTSYLTDCETQLSQAPRQGLLYGVPVS
LKECFSYKGHASTLGLSLNEGVTSESDCVVVQVLKLQGAVPFVHTNVPQSMLSYDCSNPLFGQTMNPWKP
SKSPGGSSGGEGALIGSGGSPLGLGTDIGGSIRFPSAFCGICGLKPTGNRLSKSGLKSCVYGQTAVQLSV
GPMARDVDSLALCMKALLCEDLFRLDSTIPPLPFREEIYRSSRPLRVGYYETDNYTMPTPAMRRAVMETK
QSLEAAGHTLVPFLPNNIPYALEVLSAGGLFSDGGCSFLQNFKGDFVDPCLGDLVLVLKLPRWFKKLLSF
LLKPLFPRLAAFLNSMCPRSAEKLWELQHEIEMYRQSVIAQWKAMNLDVVLTPMLGPALDLNTPGRATGA
ISYTVLYNCLDFPAGVVPVTTVTAEDDAQMEHYKGYFGDMWDNILKKGMKKGIGLPVAVQCVALPWQEEL
CLRFMREVERLMTPEKRPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010173
ORF Size 1737 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010173.1, NM_010173.2, NM_010173.3, NM_010173.4, NP_034303.2
RefSeq Size 3816 bp
RefSeq ORF 1740 bp
Locus ID 14073
UniProt ID O08914
Cytogenetics 4 53.08 cM
MW 63.2 kDa
Summary Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010173" in other vectors (4)
SKU Description Size Price
MC200558 Faah (untagged) - Mouse fatty acid amide hydrolase (Faah), (10ug) 10 ug
$540.00
MG209084 Faah (tGFP-tagged) - Mouse fatty acid amide hydrolase (Faah) 10 ug
$489.00 $740.00
MR209084L3 Lenti ORF clone of Faah (Myc-DDK-tagged) - Mouse fatty acid amide hydrolase (Faah) 10 ug
$840.00
MR209084L4 Lenti ORF clone of Faah (mGFP-tagged) - Mouse fatty acid amide hydrolase (Faah) 10 ug
$840.00

Plasmid Map for MR209084

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209084
Circular map for MR209084

file-alt Citations

Cooperative enzymatic control of N-acyl amino acids by PM20D1 and FAAH
Kim, Terrell, Li et al
Elife (2020) 9 DOI: 10.7554/eLife.55211
AI Summary: OriGene products—specifically FAAH-flag (MR209084), ACY1-flag (MR206415), CNDP1-flag (MR219018), and CNDP2-flag (MR207616) transfected constructs—were used to express and study the roles of these enzymes in the cooperative enzymatic regulation of N-acyl amino acids in this research.
Snippet: Mouse line (M. musculus) C57BL/6J Jackson Labs 000664 Transfected construct (M. musculus) PM20D1-flag Addgene 84566 Transfected construct (M. musculus) FAAH-flag Origene MR209084 Transfected construct (M. musculus) ACY1-flag Origene MR206415 Transfected construct (M. musculus) CNDP1-flag Origene MR219018 Transfected construct (M. musculus) CNDP2-flag Origene MR207616
FigureFigureFigure
FAAH genetic variation enhances fronto-amygdala function in mouse and human
Dincheva, Drysdale, Hartley et al
Nat Commun (2015) 6, 6395 DOI: 10.1038/ncomms7395
Cited by 292
AI Summary: OriGene’s mouse FAAH complementary DNA clone (NM_010173) was used as a positive control in transfection experiments to validate FAAH expression analyses in this study.
Snippet: As a positive control, 293T cells purchased from American Type Culture Collection were transfected with 1 mg mouse ​FAAH complementaryDNA clone (Origene, NM_010173), and lysates were processed in parallel. Forebrain lysate from ​FAAH knockout mice5 were used as a negative control
Showing 1-2 of 2 papers.
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