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4432405B04Rik (BC058375) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209082
4432405B04Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $539.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 4432405B04Rik
Synonyms 4432405B04Rik; Tect2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209082 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTAGTTTCAGCCTCCCACAATGCATCCTGCTTAGCACATCTGCTCATCCAGGTGGAGATTTATC
CCAACACATCTGTAACCCACAACGCATCAGAGAACATGACTGTCATTCCTAACCAAGTCTATCAGCCCCT
GGGTCCCTGCCCGTGTGACTTAACAGCCAAGGCCTGTGACATTCAATGCTGCTGTGACCAGGACTGTCAG
CCAGAAGTAAGAGAGCTGTTTGCACAGTCCTGCTTCTCCGGCGTGTTTGGAGGGCACGTCAGCCCTCCGT
CCCATCATCACTGCGCTGTCAGCACTACTCACCAGACTCCTGACTGGTTTCCCCTCCTGTGTGTGCAGTC
GCCACCCTCTACCTCGCCGTTCCTTGGTCACTTCTACCACGGTGCCACTTCCCCCAGACATTCCCCCGGC
TTTGAAGCCCATTTGCACTTCGACCTAAGAGACTTCGCGGATGCCAGTTACAAGCAAGGAGATCCCATTA
TGACCACGGAGGGGTATTTTACCATCCCCCAGGTGTCTCTAGCTGGGCAGTGTCTACAGGATGCTCCTGT
GGCTTTCCTGAGGAATTTTGACAGTGTATGTACCATGGACCTGGAAGTGCACCAAGGACGAGATGAAATT
GTCCTCGAGAACATGAAAATCCGCACAACAGGAGGTCCGGTCACACCAACAGTAACCTATGAGGAAGCCA
TTGACCTGGACAAGTTCATCACCAGCCCGGACACAGTTTTAAGTGTCGGATCGGCCCCCAGAAACGTGAA
TGTGGAAGAACATTATGTCTTCAGATGGCAAAATAATTCCATCAGCGGCCTAGACATCACAGTCATCAGG
GCAGAAATCAGTGCCCAGCAGAGAGGAATGATGACCCAGAGATTCACAGTGAAGTTTTTAAGCCATCACA
GTGGTGGTGAGAAGGAATTTTCTGGAAATCCAGGTTACCAGCTTGGCAAGCCGGTCCGAGCCCTGCATAC
CGCTGGGATGAACGTCAGCACACTGCACCTCTGGCAGCCAGCTGGAAGGGGCCTGTGCACAGCAGCCGCT
CTCAGACCCATTTTGTTTGGAGAAAATGCCTTCTCTGGCTGCCTGCTGGAAGTTGGGATTAAGGAGAACT
GTACCCAGCTCAGAGAGAATGTCCTTCAGAGACTGGATTTGCTGACACAAGCAACGCACGTGGCGAGGAG
AGGCAACTCGGACTACAGTGATCTGAGTGACGGCTGGCTGGAGATCATACGTGCCGAAGCCCCTGATACA
GGTGCCGACCTGCCCTTGAGCAGCGTGAATGGCGTGTGCCCAGAAGTTCCAGCCCGGCTCTCCATCCGCA
TTCTCACTGCTGAAGCTGGCTCTGTGGAAGGGGTGGCTCAGCGGGAGATCCTGGCTGTGGAGACAAGGTT
TTCCACAGTGACCTGGCAGTACCAGTGTGGGCTCACTTGTGAGGACAAGGCCGACCTTCTCCCTCTCAGT
GCCTCTGTTGAGTTCATTAATGTCCCTGCGCAGATGCCCCACCCCCCTACGAGATTCCAGATCAACTTCA
CAGAGTACGACTGTACCCGGAATGAGCTGTGTTGGCCCCAACTTCTGTACCCTCTGACCCAGTATTATCA
AGGGGAGCCCCAGTCTCAGTGTGTGGCCAAAGGCCTGATGCTGCTGTCACTCCTCATGCTGGCCATCCTC
CTCAGGCACCCCTGGGTCAGGATGTGCAAAGCCCGGGACTCAGCTGCCATCTACCAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209082 protein sequence
Red=Cloning site Green=Tags(s)

MLLVSASHNASCLAHLLIQVEIYPNTSVTHNASENMTVIPNQVYQPLGPCPCDLTAKACDIQCCCDQDCQ
PEVRELFAQSCFSGVFGGHVSPPSHHHCAVSTTHQTPDWFPLLCVQSPPSTSPFLGHFYHGATSPRHSPG
FEAHLHFDLRDFADASYKQGDPIMTTEGYFTIPQVSLAGQCLQDAPVAFLRNFDSVCTMDLEVHQGRDEI
VLENMKIRTTGGPVTPTVTYEEAIDLDKFITSPDTVLSVGSAPRNVNVEEHYVFRWQNNSISGLDITVIR
AEISAQQRGMMTQRFTVKFLSHHSGGEKEFSGNPGYQLGKPVRALHTAGMNVSTLHLWQPAGRGLCTAAA
LRPILFGENAFSGCLLEVGIKENCTQLRENVLQRLDLLTQATHVARRGNSDYSDLSDGWLEIIRAEAPDT
GADLPLSSVNGVCPEVPARLSIRILTAEAGSVEGVAQREILAVETRFSTVTWQYQCGLTCEDKADLLPLS
ASVEFINVPAQMPHPPTRFQINFTEYDCTRNELCWPQLLYPLTQYYQGEPQSQCVAKGLMLLSLLMLAIL
LRHPWVRMCKARDSAAIYH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC058375
ORF Size 1737 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC058375, AAH58375
RefSeq Size 2339 bp
RefSeq ORF 1739 bp
Locus ID 67978
Cytogenetics 5 F
MW 63.9 kDa
Summary Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for hedgehog signaling transduction.UniProtKB/Swiss-Prot Function
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Documents
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"BC058375" in other vectors (4)
SKU Description Size Price
MC217847 4432405B04Rik (untagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178), (10ug) 10 ug
$594.00
MG209082 4432405B04Rik (tGFP-tagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178) 10 ug
$739.00
MR209082L3 Lenti ORF clone of 4432405B04Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178) 10 ug
$839.00
MR209082L4 Lenti ORF clone of 4432405B04Rik (mGFP-tagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178) 10 ug
$839.00

Plasmid Map for MR209082

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209082
Circular map for MR209082

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