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Pigt (BC034175) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209067
Pigt (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $538.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pigt
Synonyms CGI-06, NDAP, Ndap7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209067 representing BC034175
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCTCGGTTTGCCGTTGCGGTTACTGGTGCTGTTGCTGGTGGGACGGGGCTGCTGTGGCTGCGCGG
AGGGCCCACGCGACAGCCTGCGAGAAGAGCTCGTTATCACTCCACTGCCTTCCGGCGACGTGGCCGCCAC
ATTCCAGTTCCGCACGCGTTGGGATTCGGATCTGCAGCGGGAAGGAGTGTCCCATTACAGGCTCTTCCCT
AAAGCCCTGGGACAGCTGATCTCCAAGTACTCCCTGCGGGAGCTCCACCTGTCGTTCACGCAAGGCTTTT
GGAGGACGCGATACTGGGGGCCACCCTTCCTGCAGGCTCCGTCAGGTGCAGAGCTCTGGGTCTGGTTCCA
AGACACTGTCACCGATGTGGATAAGTCCTGGAGGGAGCTCAGCAATGTCCTCTCGGGGATCTTCTGTGCA
TCCCTCAACTTCATCGACGCCACCAATACCGTCACTCCCACCGCCTCCTTCAAACCTCTGGGACTGGCCA
ATGACACTGACGACTACTTCCTGCGCTACGCTGTGCTGCCTCGGGAGGTTGTCTGCACTGAGAACCTCAC
GCCATGGAAGAAGCTCTTGCCCTGCAGTTCCAAGGCCGGTCTCTCAGTGCTATTGAAAGCAGACCGCTTG
TTCCACACCAGTTACCACTCCCAGGCAGTGCACATCCGGCCTATCTGCAGAAATGCTCACTGTACCAGCA
TCTCCTGGGAGCTGAGGCAGACCCTTTCGGTTGTCTTTGATGCCTTCATCACAGGACAGGGGAAGAAAGA
CTGGTCCCTCTTCCGCATGTTCTCCCGGACTCTCACAGAGGCCTGTCCATTGGCGTCTCAGAGCCTAGTT
TATGTGGACATCACAGGCTACAGCCAGGATAATGAAACACTGGAGGTGAGCCCTCCCCCAACTTCCACAT
ACCAGGATGTCATTTTGGGCACCAGGAAGACCTATGCCGTCTATGACTTGTTTGACACAGCCATGATCAA
TAACTCTCGAAACCTCAACATCCAGCTCAAATGGAAGAGACCCCCAGATAATGAGGCCCTACCCGTGCCC
TTCCTGCATGCGCAGCGGTACGTGAGTGGCTATGGACTGCAGAAGGGAGAGCTGAGTACTCTGCTGTACA
ACTCCCATCCTTATCGGGCCTTCCCCGTGCTGCTGCTGGATGTCGTGCCCTGGTACCTGCGGCTGTATGT
GCACACGCTCACCATTACCTCCAAGGGCAAGGAGAATAAACCAAGTTACATCCACTACCAACCTGCTCAG
GACAGGCAGCAGCCCCACCTCCTGGAGATGCTCATTCAGCTGCCAGCCAACTCCGTCACCAAGGTCTCCA
TCCAGTTTGAACGAGCCCTGCTCAAGTGGACAGAGTACACACCAGACCCCAACCACGGCTTCTATGTCAG
CCCATCTGTCCTCAGCGCCCTTGTGCCCAGTGTGGTGGCAGCCAAACCAGTGGACTGGGAAGGGAGCCCT
CTCTTCAACACCTTGTTCCCGGTGTCTGATGGCTCCAGCTACTTTGTCCGACTCTACACAGAGCCCTTGC
TAGTGAACCTGCCCACCCCGGACTTCAGCATGCCCTACAATGTGATCTGCCTTACGTGCACGGTGGTGGC
CGTGTGCTATGGCTCCTTCTACAATCTCCTCACCCGAACCTTTCACATCGAAGAGCCCAAGTCGGGCGGC
CTGGCCAAGCGGCTGGCTAACCTCATCCGCCGTGCCCGTGGTGTGCCCCCTCTC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209067 representing BC034175
Red=Cloning site Green=Tags(s)

MPLGLPLRLLVLLLVGRGCCGCAEGPRDSLREELVITPLPSGDVAATFQFRTRWDSDLQREGVSHYRLFP
KALGQLISKYSLRELHLSFTQGFWRTRYWGPPFLQAPSGAELWVWFQDTVTDVDKSWRELSNVLSGIFCA
SLNFIDATNTVTPTASFKPLGLANDTDDYFLRYAVLPREVVCTENLTPWKKLLPCSSKAGLSVLLKADRL
FHTSYHSQAVHIRPICRNAHCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMFSRTLTEACPLASQSLV
YVDITGYSQDNETLEVSPPPTSTYQDVILGTRKTYAVYDLFDTAMINNSRNLNIQLKWKRPPDNEALPVP
FLHAQRYVSGYGLQKGELSTLLYNSHPYRAFPVLLLDVVPWYLRLYVHTLTITSKGKENKPSYIHYQPAQ
DRQQPHLLEMLIQLPANSVTKVSIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSVVAAKPVDWEGSP
LFNTLFPVSDGSSYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHIEEPKSGG
LAKRLANLIRRARGVPPL

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN BC034175
ORF Size 1734 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC034175.1
RefSeq Size 2322 bp
RefSeq ORF 1736 bp
Locus ID 78928
Cytogenetics 2 H3
MW 85.1 kDa
Summary Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC034175" in other vectors (4)
SKU Description Size Price
MC218498 Pigt (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752), (10ug) 10 ug
$593.00
MG209067 Pigt (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752) 10 ug
$738.00
MR209067L3 Lenti ORF clone of Pigt (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752) 10 ug
$838.00
MR209067L4 Lenti ORF clone of Pigt (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class T (cDNA clone MGC:36817 IMAGE:5026752) 10 ug
$838.00

Plasmid Map for MR209067

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209067
Circular map for MR209067

file-alt Citations

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