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Tkfc (NM_145496) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209059
Dak (Myc-DDK-tagged) - Mouse dihydroxyacetone kinase 2 homolog (yeast) (Dak)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $538.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tkfc
Synonyms BC021917; Dak
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209059 representing NM_145496
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTCCAAGAAGATGGTGAATTCTGTGGAAGGGTGTGCTGATGATGCCCTTGCTGGGTTAGTAGCCT
CTAACCCTGACTTGCAGCTCCTGCAAGGGCACCGTGTGGCCCTACGTTCTGACCTGGACACCCTCAAGGG
CCGGGTAGCACTTCTGTCAGGTGGAGGCTCAGGCCATGAGCCTGCCCATGCCGGTTTTATCGGGAAAGGG
ATGCTAACAGGTGTCATCGCAGGATCTGTGTTTGCCTCTCCTCCTGTGGGCAGCATCCTGGCAGCCATTA
GAGCTGTGGCCCAGGCAGGCACAGTGGGCACCCTCCTCATTGTGAAGAACTACACTGGGGATCGGCTCAA
CTTTGGACTTGCCATGGAGCAGGCCAAGGCCGAGGGCATCTCTGTGGAGATGGTGATAGTTGAGGATGAC
AGTGCCTTCACCGTCCTCAAGAAGGCAGGCCGGCGTGGCCTGTGTGGCACAGTACTTATCCACAAGGTGG
CAGGTGCTCTGGCTGAGGAAGGAATGGGGCTGGAGGAGATCACAAAGAGGGTGAGCGTGATCGCCAAGAC
CATGGGTACCCTAGGGGTGAGCTTGTCATCCTGCAGTGTCCCTGGCGCCACACACACCTTTGAGCTTGCA
GCTGATGAAATAGAGCTAGGCCTGGGGATCCATGGGGAAGCTGGTGTTCGTCGGATAAAGATAGCACCTG
TTGATCAGATTGTCACTCTTATGCTTGACCACATGACAAACACCTCCAATATATTTCATGTGCCTGTACG
GTCAGGCTCTTCAGTGGTGCTGATAGTCAACAATCTGGGTGGCCTGTCTTTCCTGGAACTGGGCATCATA
GCTGATGCTGCCATACGCTTGCTGGAGGGCCGTGGAGTGAAGGTCGCCCGTGCCCTGGTGGGCACCTTCA
TGTCAGCGCTGGAGATGCCTGGTGTGTCTCTTACTTTGATGCTTGTGGATGAACCCGTGCTGAAGCTAAT
AGATGCTGAAACTACTGCAAAAGCCTGGCCTCACATGGCCAAGGTCTCTGTGACTGGGAGGAAGCGGATT
CGGGCAGCTCCCACAGAGCCTCCAGAAGCCCCTGAAGCCACTGCAGCAGGAGGTGTAACATCAAAGCAGA
TGGCACTTGTGCTGGATCGGATATGCACCACCCTCATCGGACTGGAGGAGCACCTGAATGCCTTGGACAG
GGCTGCTGGTGATGGGGATTGTGGTTCTACCCATAGCCGTGCTGCCAAAGCCATTCAGGGCTGGCTAAAG
GAAGGCCCATCTCTAACCAGCCCTGCCCAGGTACTCTCCAGATTGTCTGTCCTGCTGCTGGAGAGGATGG
GAGGCTCATCTGGGGCGCTCTATGGCCTGTTCCTGACTGCAGCTGCCCAGCCTCTCAAGGCCAAGACTGA
CCTCCCAACCTGGTCTGCTGCCATGGATGCCGGCTTAGAGTCCATGCAGAAGTATGGAAAGGCTGCACCA
GGAGACAGGACAATGCTGGATTCTCTGTGGGCAGCAGCACAGGAGTTCCAAGCCTGGAAGAGCCCAGGGG
CCAGTCTCCTCCCAGTCCTGACTAAAGCAGTCAAGAGTGCTGAAGCTGCAGCCGAGGCCACCAAGAATAT
GGAAGCTGGTGCTGGGAGAGCTAGTTATATCAGCTCTGCACAACTAGATCAGCCAGACCCTGGAGCAGTC
GCAGCTGCTGCCATCTTCCGTGCCATCCTGGAGGTCTTGCAGACGCAGGGAGCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209059 representing NM_145496
Red=Cloning site Green=Tags(s)

MSSKKMVNSVEGCADDALAGLVASNPDLQLLQGHRVALRSDLDTLKGRVALLSGGGSGHEPAHAGFIGKG
MLTGVIAGSVFASPPVGSILAAIRAVAQAGTVGTLLIVKNYTGDRLNFGLAMEQAKAEGISVEMVIVEDD
SAFTVLKKAGRRGLCGTVLIHKVAGALAEEGMGLEEITKRVSVIAKTMGTLGVSLSSCSVPGATHTFELA
ADEIELGLGIHGEAGVRRIKIAPVDQIVTLMLDHMTNTSNIFHVPVRSGSSVVLIVNNLGGLSFLELGII
ADAAIRLLEGRGVKVARALVGTFMSALEMPGVSLTLMLVDEPVLKLIDAETTAKAWPHMAKVSVTGRKRI
RAAPTEPPEAPEATAAGGVTSKQMALVLDRICTTLIGLEEHLNALDRAAGDGDCGSTHSRAAKAIQGWLK
EGPSLTSPAQVLSRLSVLLLERMGGSSGALYGLFLTAAAQPLKAKTDLPTWSAAMDAGLESMQKYGKAAP
GDRTMLDSLWAAAQEFQAWKSPGASLLPVLTKAVKSAEAAAEATKNMEAGAGRASYISSAQLDQPDPGAV
AAAAIFRAILEVLQTQGA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145496
ORF Size 1734 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145496.1, NP_663471.1
RefSeq Size 2350 bp
RefSeq ORF 1737 bp
Locus ID 225913
UniProt ID Q8VC30
Cytogenetics 19 A
MW 59.7 kDa
Summary Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response.UniProtKB/Swiss-Prot Function
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"NM_145496" in other vectors (4)
SKU Description Size Price
MC219223 Dak (untagged) - Mouse dihydroxyacetone kinase 2 homolog (yeast) (Dak), (10ug) 10 ug
$593.00
MG209059 Dak (tGFP-tagged) - Mouse dihydroxyacetone kinase 2 homolog (yeast) (Dak) 10 ug
$738.00
MR209059L3 Lenti ORF clone of Dak (Myc-DDK-tagged) - Mouse dihydroxyacetone kinase 2 homolog (yeast) (Dak) 10 ug
$838.00
MR209059L4 Lenti ORF clone of Dak (mGFP-tagged) - Mouse dihydroxyacetone kinase 2 homolog (yeast) (Dak) 10 ug
$838.00

Plasmid Map for MR209059

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209059
Circular map for MR209059

file-alt Citations

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