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Trafd1 (NM_172275) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209046
Trafd1 (Myc-DDK-tagged) - Mouse TRAF type zinc finger domain containing 1 (Trafd1), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $537.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Trafd1 or NM_172275"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trafd1
Synonyms 1110008K06Rik; Fln29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209046 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGTTTCGAGATGACCAGGCTTCTAGGCTGTGTGACAACTGCAAGAAGGAAATTCCTGTATTTA
ATTTTACCATCCATGAAATCCACTGTCAAAGGAACATTGGTGTGTGCCCTGTCTGCAAGGAACCGTTCCC
CAAATCTGACATGGACATTCACATGGCTGCAGAGCACTGTCAGGTGACCTGTAAATGCAACAAGAAGTTG
GAGAAGAGGCAGTTAAAGCAGCATGCGGAGACAGAGTGTCCCCTGCGGCTCGCCGTCTGCCAGCACTGTG
ATCTGGAGCTTTCTGTTGTCAAGTTGAAGGAGCATGAGGATTACTGTGGAGCCCGGACAGAGCTGTGTGG
CAGCTGTGGGCGCAACGTGCTTGTGAAGGAGCTGAAGACTCACCCCGAAGTCTGTGGGAGAGTGGAGGAG
GAAAAGAGAACGGAGGCTGCCATCCCTCCGGAGGCTTACGACGAGCCCTGGAGTCAGGACAGAATCTGGA
TCGCATCCCAGCTCCTCAGACAAATCGAGGCTCTGGACCCGCCCATGAGGCTCCCTGGAAGGCCCCTGCA
AGCCTTTGAGGCAGACCCCTTCTACAGTAGGACTACCAGCCAGAGGAGCATGGCAGCCCAGTTTCCAGTT
CAAAATAATCTTTTTGAAGAACAAGAAAGGCAGGAAAGGAACAGAAGCCGGCAGTCCCCAAAGGACAGCG
CTGAGAATAACGCACACTTGGACTTCATGTTGGCCTTGAGTCTGCAGAATGAGGGACAGGCCACCAGCAT
GGTAGAGCAGGGCTTCTGGGAGTCTGTGCCTGAGGCTGATCCGGCTCGTGCTGGGCCCACATCTCTAGGT
GACATAAAGGGTGCTGCTGACGAGATTCTGCTGCCGTGTGAGTTCTGTGAGGAGCTGTACCCAGAGGAAC
TGCTCATTGACCATCAGACAAGCTGCAACCCTTCTCATGCCTTACGTTCACTCAATACGGGCAGCTCTTC
CGTCAGGGGTATGGAAGATCCTGGTACCATCTTCCAGAACTTTCTACAACAAGCAACAAGTAACCAGTTT
GACACTTTAATGGGCCTGAGCAGTTCAGCTGCTGTGGAAGACAGCATCATCATCCCCTGTGAGTTCTGTG
GGGTGCAGCTGGAAGAGGAGGTGCTGTTCTACCATCAGGACCAGTGTGACCAACGCCCAGCCACAGCAAA
CCACCGTGCAGTGGAGGGCATCCCAGCCCAGGATTCGCAGCCAGAAAACACTTCAGCAGAGCTGTCCAGG
AGGCGGGTCAAACACCAGGGAGACCTGTCATCTGGTTACATGGATGATGTCAAGCCGGAATCAGTGAAAG
GCCCCACCTACTCGATGTCTCCTAACAGGACCATGAACAATGTGGCTTCCTGCAACCGACTGTTGAACTT
ACCGTCAGGGCCCAGGTCTGACTGCCAGCGTAGCCCTCCCGGTGTGCTGAAACTCAACAACTCTGATAGC
CAGGACATCCGTGGGCAGATGCGGGGCAGCCAGAATGGGCCCATAGCATCCGGGCACGCTCCAGTGATCC
ACTCTATTCAAAATCTCTATCCAGAAAACTTTGCGCCCTCTTTTCCTCATGGATCCCCTGGGAGTGGGAG
GAGTGAAGGTGGTCGGAGCTCTCGGGTCAGCCCTGCAGCTGCTGGCTACCACAGCAGAGCAGCAAAGGCC
AAGCCTCCAAAGCAGCAGGGAGCAGGCGATGCGGAGGAGGAAGAGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209046 protein sequence
Red=Cloning site Green=Tags(s)

MAEFRDDQASRLCDNCKKEIPVFNFTIHEIHCQRNIGVCPVCKEPFPKSDMDIHMAAEHCQVTCKCNKKL
EKRQLKQHAETECPLRLAVCQHCDLELSVVKLKEHEDYCGARTELCGSCGRNVLVKELKTHPEVCGRVEE
EKRTEAAIPPEAYDEPWSQDRIWIASQLLRQIEALDPPMRLPGRPLQAFEADPFYSRTTSQRSMAAQFPV
QNNLFEEQERQERNRSRQSPKDSAENNAHLDFMLALSLQNEGQATSMVEQGFWESVPEADPARAGPTSLG
DIKGAADEILLPCEFCEELYPEELLIDHQTSCNPSHALRSLNTGSSSVRGMEDPGTIFQNFLQQATSNQF
DTLMGLSSSAAVEDSIIIPCEFCGVQLEEEVLFYHQDQCDQRPATANHRAVEGIPAQDSQPENTSAELSR
RRVKHQGDLSSGYMDDVKPESVKGPTYSMSPNRTMNNVASCNRLLNLPSGPRSDCQRSPPGVLKLNNSDS
QDIRGQMRGSQNGPIASGHAPVIHSIQNLYPENFAPSFPHGSPGSGRSEGGRSSRVSPAAAGYHSRAAKA
KPPKQQGAGDAEEEEE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172275
ORF Size 1728 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172275.1, NP_758479.1
RefSeq Size 2486 bp
RefSeq ORF 1731 bp
Locus ID 231712
UniProt ID Q3UDK1
Cytogenetics 5 F
MW 63.9 kDa
Summary Negative feedback regulator that controls excessive innate immune responses. Regulates both Toll-like receptor 4 (TLR4) and DDX58/RIG1-like helicases (RLH) pathways. May inhibit the LTR pathway by direct interaction with TRAF6 and attenuation of NF-kappa-B activation. May negatively regulate the RLH pathway downstream from MAVS and upstream of NF-kappa-B and IRF3.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172275" in other vectors (4)
SKU Description Size Price
MC201435 Trafd1 (untagged) - Mouse TRAF type zinc finger domain containing 1 (Trafd1), transcript variant 2, (10ug) 10 ug
$537.00
MG209046 Trafd1 (tGFP-tagged) - Mouse TRAF type zinc finger domain containing 1 (Trafd1) 10 ug
$737.00
MR209046L3 Lenti ORF clone of Trafd1 (Myc-DDK-tagged) - Mouse TRAF type zinc finger domain containing 1 (Trafd1), transcript variant 2 10 ug
$837.00
MR209046L4 Lenti ORF clone of Trafd1 (mGFP-tagged) - Mouse TRAF type zinc finger domain containing 1 (Trafd1), transcript variant 2 10 ug
$837.00

Plasmid Map for MR209046

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file-download TXT (FASTA format)

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Cloning scheme for MR209046
Circular map for MR209046

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