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Trafd1 (NM_172275) Mouse Untagged Clone

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SKU
MC201435
Trafd1 (untagged) - Mouse TRAF type zinc finger domain containing 1 (Trafd1), transcript variant 2, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $537.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Trafd1
Synonyms 1110008K06Rik; Fln29
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC038396 sequence for NM_172275
GGGCGGCGGAGGCGGCGGTGGCGGTGCTGTTCCCGCTGGGCTCGGAGTTTTCAGGAAGAGCTGAGATGGC CGAGTTTCGAGATGACCAGGCTTCTAGGCTGTGTGACAACTGCAAGAAGGAAATTCCTGTATTTAATTTT ACCATCCATGAAATCCACTGTCAAAGGAACATTGGTGTGTGCCCTGTCTGCAAGGAACCGTTCCCCAAAT CTGACATGGACATTCACATGGCTGCAGAGCACTGTCAGGTGACCTGTAAATGCAACAAGAAGTTGGAGAA GAGGCAGTTAAAGCAGCATGCGGAGACAGAGTGTCCCCTGCGGCTCGCCGTCTGCCAGCACTGTGATCTG GAGCTTTCTGTTGTCAAGTTGAAGGAGCATGAGGATTACTGTGGAGCCCGGACAGAGCTGTGTGGCAGCT GTGGGCGCAACGTGCTTGTGAAGGAGCTGAAGACTCACCCCGAAGTCTGTGGGAGAGTGGAGGAGGAAAA GAGAACGGAGGCTGCCATCCCTCCGGAGGCTTACGACGAGCCCTGGAGTCAGGACAGAATCTGGATCGCA TCCCAGCTCCTCAGACAAATCGAGGCTCTGGACCCGCCCATGAGGCTCCCTGGAAGGCCCCTGCAAGCCT TTGAGGCAGACCCCTTCTACAGTAGGACTACCAGCCAGAGGAGCATGGCAGCCCAGTTTCCAGTTCAAAA TAATCTTTTTGAAGAACAAGAAAGGCAGGAAAGGAACAGAAGCCGGCAGTCCCCAAAGGACAGCGCTGAG AATAACGCACACTTGGACTTCATGTTGGCCTTGAGTCTGCAGAATGAGGGACAGGCCACCAGCATGGTAG AGCAGGGCTTCTGGGAGTCTGTGCCTGAGGCTGATCCGGCTCGTGCTGGGCCCACATCTCTAGGTGACAT AAAGGGTGCTGCTGACGAGATTCTGCTGCCGTGTGAGTTCTGTGAGGAGCTGTACCCAGAGGAACTGCTC ATTGACCATCAGACAAGCTGCAACCCTTCTCATGCCTTACGTTCACTCAATACGGGCAGCTCTTCCGTCA GGGGTATGGAAGATCCTGGTACCATCTTCCAGAACTTTCTACAACAAGCAACAAGTAACCAGTTTGACAC TTTAATGGGCCTGAGCAGTTCAGCTGCTGTGGAAGACAGCATCATCATCCCCTGTGAGTTCTGTGGGGTG CAGCTGGAAGAGGAGGTGCTGTTCTACCATCAGGACCAGTGTGACCAACGCCCAGCCACAGCAAACCACC GTGCAGTGGAGGGCATCCCAGCCCAGGATTCGCAGCCAGAAAACACTTCAGCAGAGCTGTCCAGGAGGCG GGTCAAACACCAGGGAGACCTGTCATCTGGTTACATGGATGATGTCAAGCCGGAATCAGTGAAAGGCCCC ACCTACTCGATGTCTCCTAACAGGACCATGAACAATGTGGCTTCCTGCAACCGACTGTTGAACTTACCGT CAGGGCCCAGGTCTGACTGCCAGCGTAGCCCTCCCGGTGTGCTGAAACTCAACAACTCTGATAGCCAGGA CATCCGTGGGCAGATGCGGGGCAGCCAGAATGGGCCCATAGCATCCGGGCACGCTCCAGTGATCCACTCT ATTCAAAATCTCTATCCAGAAAACTTTGCGCCCTCTTTTCCTCATGGATCCCCTGGGAGTGGGAGGAGTG AAGGTGGTCGGAGCTCTCGGGTCAGCCCTGCAGCTGCTGGCTACCACAGCAGAGCAGCAAAGGCCAAGCC TCCAAAGCAGCAGGGAGCAGGCGATGCGGAGGAGGAAGAGGAGTGATGGTGACTCTGGGGGCTCTTGCAG CCCCCACCCCACCCCACCCCATGCATAACAGAACATGCTGGTGCAATGTCCAGCTGCCTCTCGGGCTTAC TGGGCAGGAGAGACACCAGATTGTAATTTTTCCTAGGTTATGGCCATTTTGTGTCTTTGCGATTGTGCTG TGTGTGTTTGGGTTTGAGGGAGGGCTAGCTGGGAGGCCCGAGCTGCCATTGTTGAGCAGCCTTGAAATCA GCGCTGAGTGTCTGTGGCCCTCCTGGCCCATAAGGGCTCCATCCTTAGTGATGTCATCACCACTGCTTCT TGGTGCTTTGTGGTCCTGATGGACAGAGACAAGGGTTCTGGGATCTCAGAGTGAGAAGCATCCTCCTGGT GGGGACCTAAGCTTGAGTGTGCAGCGTGTTACACAGGCACATGTCTGGCAGGGACACGGACTGGAGGCCA CCTTCGTCCTTGCGGGTCACTCACCCACCTCTCTCTGTTCTTGATGAGGAATGTACTGCTTTGTTCATGC TGCCCCTACCAGGGCCACTCCTACTCATGAGGACGGAGGCGCCAGCCCCTCCCCTTTTTATTTTACTGTT CTAATTGTCTATTTCCTTGTAATAGAAATAAAGTTTGTATTTAGAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_172275
Insert Size 1731 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC038396, AAH38396
RefSeq Size 2449 bp
RefSeq ORF 1731 bp
Locus ID 231712
UniProt ID Q3UDK1
Cytogenetics 5 F
Summary Negative feedback regulator that controls excessive innate immune responses. Regulates both Toll-like receptor 4 (TLR4) and DDX58/RIG1-like helicases (RLH) pathways. May inhibit the LTR pathway by direct interaction with TRAF6 and attenuation of NF-kappa-B activation. May negatively regulate the RLH pathway downstream from MAVS and upstream of NF-kappa-B and IRF3.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, that results in a protein (isoform 2) with a shorter C-terminus, compared to isoform 1. Variants 2 and 3 both encode the same isoform (2).
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Documents
Resources
"NM_172275" in other vectors (4)
SKU Description Size Price
MG209046 Trafd1 (tGFP-tagged) - Mouse TRAF type zinc finger domain containing 1 (Trafd1) 10 ug
$737.00
MR209046 Trafd1 (Myc-DDK-tagged) - Mouse TRAF type zinc finger domain containing 1 (Trafd1), transcript variant 2 10 ug
$289.00 $537.00
MR209046L3 Lenti ORF clone of Trafd1 (Myc-DDK-tagged) - Mouse TRAF type zinc finger domain containing 1 (Trafd1), transcript variant 2 10 ug
$837.00
MR209046L4 Lenti ORF clone of Trafd1 (mGFP-tagged) - Mouse TRAF type zinc finger domain containing 1 (Trafd1), transcript variant 2 10 ug
$837.00

file-alt Citations

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