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Coil (BC031167) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208968
Coil (Myc-DDK-tagged) - Mouse coilin (cDNA clone MGC:37472 IMAGE:4983919)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $530.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Coil
Synonyms Cln80, p80-coilin, MGC37472
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208968 representing BC031167
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCTCCGAGACGGTTAGGCTACGGCTTCAATTTGACTACCCGCCGCCGGCCACTCCACACTGCA
CGGTCTTCTGGCTTCTGGTGGACCTGAACAGATGTCGAGTTGTCACGGATCTCATCAGTCTCATCCGCCA
GCGCTTCGGCTTCAGTTCCGGGGCGCTCCTGGGCCTCTATCTGGAAGGGGGGCTGCTGCCCCCTGCCGAG
AGCGCGCGCCTGGTGAGAGACAACGACAGCCTCAGGGTTAAGTTAGAAGACCAAGGACTCCCTGAGAACC
TGATAGTAAGCAATGGTGGCGACTCTAGTTTCCCATGCAGGAAAGCAAAGAAACGGGCTTTTAAGTTGAT
GGAGGATGAGGAGACAGATCAGGGATACAAGTCTTCGAAGAAGCACTGCAAGAGGCAGGAGGACAGCGGC
CAGAATGAGAAGGCCTCGGACCTGGAAACTAAACTCCTCCCAGATGAGACGGGGCGGACAAAGAGCAAGA
AAAAGAGCAAAGTGACAGGCAGCCCAGCCGAAGAGGACGAGGAAGAGACCAAAAAGAAGTCTTCAAAAAG
AAAGGAGAAGTGTGAACCGAAGAAGCAGACCAGGGCCTCCAAGTCTTCCAAACAACAGACGCCTAAAGAG
GGGGCCTCACAGAACTGCAGCTTCCCCAGGGCCTCCCCCAGAAGCCTCGGTAAAGCCCGGAGGAGAAGCA
GCACGGGGCTAAAAGGTAGCTCAGGCCACCTGTCAGAGTCTGAGTCCTGCCCAGAGTCAGTCAGCGATGG
CCACTGCAGTGTCATGATGCAGGAGGTCACCTTTTCAGAGAAACTGTCAGCTGAGCTGTTGAAAGATGCA
CCTGCTACTAAGACTGCAGCTGCCAACAGGCAGGCCTCAAAGCCTGGCTTTACCTTCAGCTCCGGCAAGG
GCAAGGCCTCCAGAACCTCGTCATCTAGCTCAGACTCCAGTTCAGAGTCGGAAGACCAGTTCCTGGTGTC
CAAGAACATGCTCGAGGGCGCATCTGGCTTCTTAAAGCCAACTGGCCTCTTTGCAGGGCAAGGGGGTTCT
GGTCCAGGGCTGTCATTGGAGACTCCGGGTATTATGGGATGGAAGTCTTCTGACTCAAACCGGGGCAGAC
AAGCTCCTGGTCCTCCCAGTACGCCTGTCCCCACCAGTTTGGGAAGAGGATGGGGGCGAGGAGAGGACTT
GTTGTTTGGGAAGGGACTGAGGGGCCGGGGTGTTCGGGGCCGAGGGCGAGGGCGCGGGCAAGCGGTCTCC
TGTGTCTTTAATAGAAGCTCTGAGAGTCAGAAGCAGAGACAGTTAAACGATATTCTAACAAACTCATCTG
TGGTGATCCAGAATCCAGTAGAGCCCCCCAAGAAGGACTATAGTCTATTACCACTGTTGGCAGCTGCCCC
TCAAGTCGGAGAAAAGATTGCATTTAAGCTTTTGGAACTCACATCCGACTACTCTCCTGATGTCTCTGAC
TACAAGGAAGGAAAAATATTAAGTCACGATCCAGAGACCCAGCAAGTGGACATAGAGGTCCTCTCCTCGC
TACCGGCTTTGAAAGAACCTGGGAAGTTTGATTTGGTCTATCACAATGAAAACGGAACCGAGGTGGTGGA
ATACGCTGTGACTCAGGAGAAGAGGATCACCGTGCTCTGGAGAGAGTTGATCGATCCGAGGCTGATCATT
GACTCTTCAGGTAGCATCTCGAGTACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208968 representing BC031167
Red=Cloning site Green=Tags(s)

MAASETVRLRLQFDYPPPATPHCTVFWLLVDLNRCRVVTDLISLIRQRFGFSSGALLGLYLEGGLLPPAE
SARLVRDNDSLRVKLEDQGLPENLIVSNGGDSSFPCRKAKKRAFKLMEDEETDQGYKSSKKHCKRQEDSG
QNEKASDLETKLLPDETGRTKSKKKSKVTGSPAEEDEEETKKKSSKRKEKCEPKKQTRASKSSKQQTPKE
GASQNCSFPRASPRSLGKARRRSSTGLKGSSGHLSESESCPESVSDGHCSVMMQEVTFSEKLSAELLKDA
PATKTAAANRQASKPGFTFSSGKGKASRTSSSSSDSSSESEDQFLVSKNMLEGASGFLKPTGLFAGQGGS
GPGLSLETPGIMGWKSSDSNRGRQAPGPPSTPVPTSLGRGWGRGEDLLFGKGLRGRGVRGRGRGRGQAVS
CVFNRSSESQKQRQLNDILTNSSVVIQNPVEPPKKDYSLLPLLAAAPQVGEKIAFKLLELTSDYSPDVSD
YKEGKILSHDPETQQVDIEVLSSLPALKEPGKFDLVYHNENGTEVVEYAVTQEKRITVLWRELIDPRLII
DSSGSISST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC031167
ORF Size 1707 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC031167.1
RefSeq Size 2653 bp
RefSeq ORF 1709 bp
Locus ID 12812
Cytogenetics 11 54.34 cM
MW 97.2 kDa
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"BC031167" in other vectors (4)
SKU Description Size Price
MC218152 Coil (untagged) - Mouse coilin (cDNA clone MGC:37472 IMAGE:4983919), (10ug) 10 ug
$584.00
MG208968 Coil (tGFP-tagged) - Mouse coilin (cDNA clone MGC:37472 IMAGE:4983919) 10 ug
$730.00
MR208968L3 Lenti ORF clone of Coil (Myc-DDK-tagged) - Mouse coilin (cDNA clone MGC:37472 IMAGE:4983919) 10 ug
$830.00
MR208968L4 Lenti ORF clone of Coil (mGFP-tagged) - Mouse coilin (cDNA clone MGC:37472 IMAGE:4983919) 10 ug
$830.00

Plasmid Map for MR208968

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208968
Circular map for MR208968

file-alt Citations

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