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C9 (NM_013485) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208897
C9 (Myc-DDK-tagged) - Mouse complement component 9 (C9)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $523.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol C9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208897 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTTGCAAGCCTCCAGGGCTCCCCAGGAGGAGCAGCATGGCCTCAGGCATGGCCATCACCTTAGCCC
TTGCCATCTTTGCCTTGGGTGTCAATGCACAGATGCCAATACCCGTTTCCAGAGAAGAACAAGAACAACA
CTATCCCATACCGATAGACTGCAGAATGAGCCCATGGAGCAATTGGTCAGAGTGTGATCCTTGCCTCAAA
CAAAGGTTTCGCTCAAGAAGCATTTTAGCCTTCGGACAGTTTAATGGGAAAAGCTGTGTTGATGTTTTGG
GAGACAGACAAGGCTGTGAACCCACCCAGGAGTGTGAAGAGATACAGGAAAACTGTGGAAATGACTTTCA
GTGTGAGACAGGCAGGTGCATAAAGAGGAGACTTCTGTGTAATGGTGACAACGACTGTGGAGATTATTCT
GATGAGAATGACTGTGACGATGACCCACGCACCCCATGCCGTGACCGAGTAGCGGAAGAATCAGAGCTGG
GACTAACAGCAGGCTATGGGATCAACATCTTAGGGATGGAGCCCCTGAGAACACCTTTTGACAATGAGTT
CTACAACGGACTCTGTGACCGGGTACGAGACGAAAAGACATACTATCGCAAACCTTGGAATGTAGTTTCT
CTGATCTATGAAACCAAGGCTGATAAAAGTTTCAGAACTGAGAACTATGACGAACACTTGGAAGTATTCA
AAGCCATCAACCGAGAGAAGACCTCGAATTTTAATGCAGATTTTGCCCTAAAATTTTCAGCCACCGAAGT
ACCTGAAAAGGGAGCTGGGGAAGTCTCCCCAGCAGAACACTCTTCAAAACCTACAAACATTTCAGCTAAA
TTTAAATTTTCATATTTCATGGGAAAAAATTTTCGAAGACTATCATCTTATTTTTCGCAGTCGAAAAAGA
TGTTTGTGCACTTGAGAGGAGTGGTCCAACTGGGGAGATTTGTAATGAGGAATCGGGATGTTGTGCTGAG
GTCAACTTTCCTGGATGATGTAAAAGCTCTACCAACTTCCTATGAAAAGGGAGAATATTTTGGATTTTTG
GAAACCTATGGGACTCACTACAGTACCTCTGGGTCCCTGGGAGGACAATATGAAATTGTCTATGTCTTGG
ATAAAGCTTCCATGAAAGAGAAAGGTGTTGACCTGAATGATGTAAAACATTGTCTTGGATTTAATATGGA
TTTACGTATTCCTCTACAAGACGACTTAAAGGATGCATCAGTCACAGCAAGTGTTAATGCGGATGGTTGC
ATAAAGACAGATAATGGGAAAACTGTAAACATCACCCGCGATAACATCATAGATGATGTCATTTCATTCA
TAAGAGGAGGGACTAGGGAGCAAGCAATTCTCCTGAAAGAGAAGATTCTCAGAGGAGACAAGACATTTGA
TAAGACTGACTTCGCCAACTGGGCCTCGTCCCTGGCAAACGCTCCAGCTCTCATCAGTCAAAGAATGTCC
CCTATATATAATCTCATTCCTTTGAAAATAAAAGATGCATACATAAAGAAGCAAAATTTGGAAAAGGCTG
TTGAAGACTATATAGATGAATTCAGTACTAAAAGGTGCTACCCATGTCTAAATGGAGGTACTATAATTCT
TCTGGATGGGCAGTGCCTGTGCTCCTGCCCAATGATGTTTAGGGGAATGGCCTGCGAAATCCATCAAAAA
ATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208897 protein sequence
Red=Cloning site Green=Tags(s)

MLCKPPGLPRRSSMASGMAITLALAIFALGVNAQMPIPVSREEQEQHYPIPIDCRMSPWSNWSECDPCLK
QRFRSRSILAFGQFNGKSCVDVLGDRQGCEPTQECEEIQENCGNDFQCETGRCIKRRLLCNGDNDCGDYS
DENDCDDDPRTPCRDRVAEESELGLTAGYGINILGMEPLRTPFDNEFYNGLCDRVRDEKTYYRKPWNVVS
LIYETKADKSFRTENYDEHLEVFKAINREKTSNFNADFALKFSATEVPEKGAGEVSPAEHSSKPTNISAK
FKFSYFMGKNFRRLSSYFSQSKKMFVHLRGVVQLGRFVMRNRDVVLRSTFLDDVKALPTSYEKGEYFGFL
ETYGTHYSTSGSLGGQYEIVYVLDKASMKEKGVDLNDVKHCLGFNMDLRIPLQDDLKDASVTASVNADGC
IKTDNGKTVNITRDNIIDDVISFIRGGTREQAILLKEKILRGDKTFDKTDFANWASSLANAPALISQRMS
PIYNLIPLKIKDAYIKKQNLEKAVEDYIDEFSTKRCYPCLNGGTIILLDGQCLCSCPMMFRGMACEIHQK
I

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013485
ORF Size 1683 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013485.2
RefSeq Size 1767 bp
RefSeq ORF 1686 bp
Locus ID 12279
UniProt ID P06683
Cytogenetics 15 A1
MW 63.4 kDa
Summary Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013485" in other vectors (4)
SKU Description Size Price
MC218917 C9 (untagged) - Mouse complement component 9 (C9), (10ug) 10 ug
$575.00
MG208897 C9 (tGFP-tagged) - Mouse complement component 9 (C9) 10 ug
$723.00
MR208897L3 Lenti ORF clone of C9 (Myc-DDK-tagged) - Mouse complement component 9 (C9) 10 ug
$823.00
MR208897L4 Lenti ORF clone of C9 (mGFP-tagged) - Mouse complement component 9 (C9) 10 ug
$823.00

Plasmid Map for MR208897

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208897
Circular map for MR208897

file-alt Citations

Differential contribution of C5aR and C5b-9 pathways to renal thrombic microangiopathy and macrovascular thrombosis in mice carrying an atypical hemolytic syndrome-related factor H mutation
Ueda, Miwa, Ito et al
Kidney Int (2019) 96 (1), 67-79 DOI: 10.1016/j.kint.2019.01.009
Cited by 42
AI Summary: In this study, OriGene's C5aR1 (NM_007693) and C9 (NM_007374) real-time PCR primer sets were used to measure gene expression, helping to elucidate the distinct roles of C5aR and C5b-9 pathways in renal and vascular pathology in a mouse model of atypical hemolytic uremic syndrome.
Figure
Showing 1-1 of 1 papers.
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