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Mon1a (NM_028369) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208816
Mon1a (Myc-DDK-tagged) - Mouse MON1 homolog A (yeast) (Mon1a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $519.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Mon1a or NM_028369"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mon1a
Synonyms 2810468K17Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208816 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTGACATGCAGAGGAAGAGAAGCAGCGAATGCCCCGAGGGCACACTGGCTCCTTCCAATGGCC
AAAGTGTGGAAAGAGCTGAGAGCCCCACGCCAGGACTAACCCAGGGAACGGAGCCAGGTGCTGGGCAGGA
GGGTGCGATGTTCGTCCATACACGTTCCTATGAAGACCTGACAGAGTTAGAAGACAGAGAAGCTTCTGGG
GACAGCCCCAAAGAGTGCGTTGGGAGTCCCCCACCACTGGCTACGGACATGCGCCAGATTAGCCAGGACT
TCAGCGAGCTAAGCACCCAGCTGACCGGTGTGGCCCGGGACCTGCAGGAGGAAATGCTGCCAGGAAGCTC
TGAAGACTGGCCGGAGCCCCAGGGCGCAGCTGGCCGAGGAGCAGCCACAGAGCCCTCCCAAGAAGGCTCA
ACTGAAGGGGAAGAGGAAGACGCTACGGAGGCATGGCGTCTCCACCAGAAGCATGTGTTTGTACTGAGTG
AGGCAGGGAAGCCTGTGTATTCTCGCTATGGGTCTGAGGAAGCCCTTTCCAGCACTATGGGTGTCATGGT
TGCCCTGGTGTCCTTCCTGGAGGCAGACAAGAATGCCATCCGCTCCATCCATGCAGATGGCTACAAGGTA
GTATTCGTGCGCCGGAGCCCTCTGGTGCTGGTGGCGGTGGCCCGCACGCGGCAGTCGGCCCAGGAGCTGG
CGCAGGAGCTACTGTACATCTACTACCAGATCCTGAGCCTTCTTACCGGCGCGCAGCTGAGCCACATCTT
CCAGCAGAAGCAGAACTACGACCTGCGGCGCCTGCTCTCGGGCTCGGAGCGAATCACCGATAACCTGCTG
CAGCTAATGGCTCGAGACCCCAGTTTCCTGATGGGCGCGGCGCGGTGCCTGCCTTTGGCAGCGGCCGTGC
GGGACACGGTGAGTGCCAGCCTGCAGCAGGCGCGTGCTCGCAGCCTGGTCTTTTCCATCCTGCTGGCCCA
TAACCAGCTGGTGGCGCTCGTGCGCCGCAAGGACCAATTCCTTCATCCCATCGACCTGCACCTGCTTTTC
AACCTCATTAGCTCCTCCTCCTCCTTCCGTGAGGGCGAGGCTTGGACCCCAGTGTGCCTGCCAAAGTTCA
ATGCGGCCGGCTTCTTCCACGCACACATCTCTTACCTAGAACCTGACACCGACCTCTGCCTGCTGCTGAT
CTCCACTGACCGTGAGGACTTCTTTGCAGTCTCTGACTGCCGACGGCGCTTCCAGGAACGCCTCCGAAAG
CGGGGAACCCATTTGGCCCTGCGGGAGGCGCTGCGCACACCTTACTACAGCGTTGCCCAAGTGGGCATCC
CTGACCTTCGCCACTTCCTCTATAAATCAAAGAGCTCAGGACTCTTCACCAGTCCTGAGATTGAGGCCCC
GTACTCCAGTGAGGAGGAGCAGGAGCGACTGTTGGGCCTCTACCAGTACCTGCATAGCCGTGCCCACAAT
GCCTCTCGCCCACTCAAGACCATCTACTACACAGGCCCCAATGAGAACCTCCTAGCCTGGGTGACAGGTG
CCTTTGAACTCTACATGTGCTACAGCCCACTGGGGACCAAGGCATCAGCTGTCAGCGCCATCCATAAGCT
GATGCGCTGGATCCGTAAAGAGGAAGACCGTCTCTTCATCCTCACGCCCCTCACCTAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208816 protein sequence
Red=Cloning site Green=Tags(s)

MAADMQRKRSSECPEGTLAPSNGQSVERAESPTPGLTQGTEPGAGQEGAMFVHTRSYEDLTELEDREASG
DSPKECVGSPPPLATDMRQISQDFSELSTQLTGVARDLQEEMLPGSSEDWPEPQGAAGRGAATEPSQEGS
TEGEEEDATEAWRLHQKHVFVLSEAGKPVYSRYGSEEALSSTMGVMVALVSFLEADKNAIRSIHADGYKV
VFVRRSPLVLVAVARTRQSAQELAQELLYIYYQILSLLTGAQLSHIFQQKQNYDLRRLLSGSERITDNLL
QLMARDPSFLMGAARCLPLAAAVRDTVSASLQQARARSLVFSILLAHNQLVALVRRKDQFLHPIDLHLLF
NLISSSSSFREGEAWTPVCLPKFNAAGFFHAHISYLEPDTDLCLLLISTDREDFFAVSDCRRRFQERLRK
RGTHLALREALRTPYYSVAQVGIPDLRHFLYKSKSSGLFTSPEIEAPYSSEEEQERLLGLYQYLHSRAHN
ASRPLKTIYYTGPNENLLAWVTGAFELYMCYSPLGTKASAVSAIHKLMRWIRKEEDRLFILTPLTY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028369
ORF Size 1668 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028369.1, NM_028369.2, NM_028369.3, NP_082645.1
RefSeq Size 2112 bp
RefSeq ORF 1671 bp
Locus ID 72825
UniProt ID Q6PDG8
Cytogenetics 9 F1
MW 62.1 kDa
Summary Plays an important role in membrane trafficking through the secretory apparatus. Not involved in endocytic trafficking to lysosomes (PubMed:17632513). Acts in concert with CCZ1, as a guanine exchange factor (GEF) for RAB7, promotes the exchange of GDP to GTP, converting it from an inactive GDP-bound form into an active GTP-bound form (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028369" in other vectors (4)
SKU Description Size Price
MC202312 Mon1a (untagged) - Mouse MON1 homolog A (yeast) (Mon1a), (10ug) 10 ug
$519.00
MG208816 Mon1a (tGFP-tagged) - Mouse MON1 homolog A (yeast) (Mon1a) 10 ug
$719.00
MR208816L3 Lenti ORF clone of Mon1a (Myc-DDK-tagged) - Mouse MON1 homolog A (yeast) (Mon1a) 10 ug
$819.00
MR208816L4 Lenti ORF clone of Mon1a (mGFP-tagged) - Mouse MON1 homolog A (yeast) (Mon1a) 10 ug
$819.00

Plasmid Map for MR208816

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208816
Circular map for MR208816

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