Cpsf6 (NM_001013391) Mouse Tagged ORF Clone

SKU MR208746

Cpsf6 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Cpsf6
• Synonyms: 4733401N12Rik; AI256641; CFIM; CFIM68; HPBRII-4; HPBRII-7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208746 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGACGGTGTGGACCACATAGACATTTACGCGGATGTGGGTGAAGAGTTCAACCAGGAAGCAGAAT; ATGGTGGACATGATCAGATAGATTTGTATGATGATGTCATCTCTCCATCTGCAAATAATGGCGATGCCCC; AGAAGATCGCGACTACATGGATACTCTTCCACCAACTGTTGGTGATGATGTGGGTAAAGGAGCCGCACCA; AACGTTGTGTACACTTACACTGGGAAGAGAATCGCATTGTATATTGGAAATCTAACATGGTGGACAACAG; ATGAGGACCTAACTGAGGCCGTTCATTCTTTGGGAGTAAATGATATTTTGGAGATAAAATTCTTTGAAAA; TCGGGCAAATGGACAATCAAAGGGATTTGCCCTTGTTGGTGTTGGATCTGAAGCATCTTCCAAAAAGTTA; ATGGATCTTTTGCCTAAAAGAGAACTTCATGGTCAGAGTCCTGTTGTAACTCCATGCAATAAGCAGTTCC; TGAGTCAATTTGAAATGCAATCCAGGAAAACTACACAGTCAGGACAGATGTCTGGGGAAGGGAAAGCCGG; TCCTCCAGGAGGCGGTTCACGCGCAGCGTTTCCTCAAGGTGGTAGAGGACGGGGCCGGTTTCCAGGGGCT; GTTCCTGGTGGGGACAGATTTCCTGGGCCAGCAGGACCAGGAGGGCCACCTCCACCTTTTCCAGCTGGAC; AAACTCCACCACGTCCACCTTTAGGCCCACCTGGCCCACCTGGTCCACCAGGTCCTCCACCTCCTGGTCA; GGTTCTGCCACCTCCTCTAGCAGGACCTCCTAACCGAGGAGACCGCCCTCCACCACCAGTTCTTTTTCCT; GGACAACCTTTTGGGCAGCCTCCGCTGGGTCCACTTCCTCCTGGGCCTCCACCTCCAGTTCCAGGCTACG; GCCCCCCTCCAGGTCCACCGCCTCCACAGCAGGGACCACCTCCACCTCCAGGCCCCTTTCCACCTCGCCC; ACCAGGTCCACTAGGGCCTCCCCTCACACTTGCTCCTCCTCCACATCTTCCGGGACCACCTCCAGGTGCC; CCGCCACCAGCTCCACATGTGAATCCTGCTTTTTTTCCTCCACCAACTAATAGTGGCATGCCAACATCAG; ATAGTCGAGGTCCACCACCAACAGACCCATATGGCCGACCTCCCCCATATGATAGGGGTGACTACGGTCC; CCCTGGGAGGGAAATGGATACTGCAAGAACACCTCTGAGTGAAGCTGAGTTTGAAGAAATCATGAATAGA; AATCGGGCAATCTCAAGCAGTGCTATTTCAAGAGCTGTGTCTGACGCTAGTGCTGGTGATTATGGGAGTG; CTATTGAAACATTGGTAACAGCAATTTCTTTAATTAAACAATCCAAAGTCTCTGCAGATGATCGTTGCAA; AGTTCTTATTAGCTCTCTGCAGGATTGCCTTCATGGAATCGAGTCCAAGTCCTATGGGTCTGGATCCAGA; CGTGAACGATCAAGAGAACGGGACCATAGTAGATCACGGGAAAAGAGTCGTCGCCATAAATCTCGGAGTA; GAGATCGCCACGATGACTATTACAGAGAGAGAAGCAGAGAACGAGAGAGACACCGGGATCGGGACCGCGA; CCGTGACCGAGAGCGTGACCGAGAGCGAGAATACCGTCATCGT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208746 protein sequence; Red=Cloning site Green=Tags(s); ; MADGVDHIDIYADVGEEFNQEAEYGGHDQIDLYDDVISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAP; NVVYTYTGKRIALYIGNLTWWTTDEDLTEAVHSLGVNDILEIKFFENRANGQSKGFALVGVGSEASSKKL; MDLLPKRELHGQSPVVTPCNKQFLSQFEMQSRKTTQSGQMSGEGKAGPPGGGSRAAFPQGGRGRGRFPGA; VPGGDRFPGPAGPGGPPPPFPAGQTPPRPPLGPPGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPPVLFP; GQPFGQPPLGPLPPGPPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRPPGPLGPPLTLAPPPHLPGPPPGA; PPPAPHVNPAFFPPPTNSGMPTSDSRGPPPTDPYGRPPPYDRGDYGPPGREMDTARTPLSEAEFEEIMNR; NRAISSSAISRAVSDASAGDYGSAIETLVTAISLIKQSKVSADDRCKVLISSLQDCLHGIESKSYGSGSR; RERSRERDHSRSREKSRRHKSRSRDRHDDYYRERSRERERHRDRDRDRDRERDREREYRHR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013391
• ORF Size: 1653 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001013391.2
• RefSeq Size: 6534 bp
• RefSeq ORF: 1656 bp
• Locus ID: 432508
• UniProt ID: Q6NVF9
• Cytogenetics: 10 D2
• MW: 59.2 kDa
• Summary: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery. Plays a role in mRNA export.[UniProtKB/Swiss-Prot Function]