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Cpsf6 (NM_001013391) Mouse Untagged Clone

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SKU
MC202653
Cpsf6 (untagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $514.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Cpsf6
Synonyms 4733401N12Rik; AI256641; CFIM; CFIM68; HPBRII-4; HPBRII-7
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC068133 sequence for NM_001013391
GCGGGCCGACCTGCAGGAGGCGGCGGCGGCGGCGGCCGAGGCCGAGGGAAGATGGCGGACGGTGTGGACC ACATAGACATTTACGCGGATGTGGGTGAAGAGTTCAACCAGGAAGCAGAATATGGTGGACATGATCAGAT AGATTTGTATGATGATGTCATCTCTCCATCTGCAAATAATGGCGATGCCCCAGAAGATCGCGACTACATG GATACTCTTCCACCAACTGTTGGTGATGATGTGGGTAAAGGAGCCGCACCAAACGTTGTGTACACTTACA CTGGGAAGAGAATCGCATTGTATATTGGAAATCTAACATGGTGGACAACAGATGAGGACCTAACTGAGGC CGTTCATTCTTTGGGAGTAAATGATATTTTGGAGATAAAATTCTTTGAAAATCGGGCAAATGGACAATCA AAGGGATTTGCCCTTGTTGGTGTTGGATCTGAAGCATCTTCCAAAAAGTTAATGGATCTTTTGCCTAAAA GAGAACTTCATGGTCAGAGTCCTGTTGTAACTCCATGCAATAAGCAGTTCCTGAGTCAATTTGAAATGCA ATCCAGGAAAACTACACAGTCAGGACAGATGTCTGGGGAAGGGAAAGCCGGTCCTCCAGGAGGCGGTTCA CGCGCAGCGTTTCCTCAAGGTGGTAGAGGACGGGGCCGGTTTCCAGGGGCTGTTCCTGGTGGGGACAGAT TTCCTGGGCCAGCAGGACCAGGAGGGCCACCTCCACCTTTTCCAGCTGGACAAACTCCACCACGTCCACC TTTAGGCCCACCTGGCCCACCTGGTCCACCAGGTCCTCCACCTCCTGGTCAGGTTCTGCCACCTCCTCTA GCAGGACCTCCTAACCGAGGAGACCGCCCTCCACCACCAGTTCTTTTTCCTGGACAACCTTTTGGGCAGC CTCCGCTGGGTCCACTTCCTCCTGGGCCTCCACCTCCAGTTCCAGGCTACGGCCCCCCTCCAGGTCCACC GCCTCCACAGCAGGGACCACCTCCACCTCCAGGCCCCTTTCCACCTCGCCCACCAGGTCCACTAGGGCCT CCCCTCACACTTGCTCCTCCTCCACATCTTCCGGGACCACCTCCAGGTGCCCCGCCACCAGCTCCACATG TGAATCCTGCTTTTTTTCCTCCACCAACTAATAGTGGCATGCCAACATCAGATAGTCGAGGTCCACCACC AACAGACCCATATGGCCGACCTCCCCCATATGATAGGGGTGACTACGGTCCCCCTGGGAGGGAAATGGAT ACTGCAAGAACACCTCTGAGTGAAGCTGAGTTTGAAGAAATCATGAATAGAAATCGGGCAATCTCAAGCA GTGCTATTTCAAGAGCTGTGTCTGACGCTAGTGCTGGTGATTATGGGAGTGCTATTGAAACATTGGTAAC AGCAATTTCTTTAATTAAACAATCCAAAGTCTCTGCAGATGATCGTTGCAAAGTTCTTATTAGCTCTCTG CAGGATTGCCTTCATGGAATCGAGTCCAAGTCCTATGGGTCTGGATCCAGACGTGAACGATCAAGAGAAC GGGACCATAGTAGATCACGGGAAAAGAGTCGTCGCCATAAATCTCGGAGTAGAGATCGCCACGATGACTA TTACAGAGAGAGAAGCAGAGAACGAGAGAGACACCGGGATCGGGACCGCGACCGTGACCGAGAGCGTGAC CGAGAGCGAGAATACCGTCATCGTTAGAAGCTGAAGGAAGAGGAACACCTTCCAAGACAAAAGAGTCTTC TCGGGGGAAAAGTGACGCTTGTCCAGCAGTTTGCTTCTTGTGATTGAACTGAACCTGTAAGGCTTCATGG ATAAACTGAACAGGAATAGATCTGAATAAAGCAAATCTGCATAAATGGTAACCAGTAGCTCTACTTTTAT TTTTTATGTTGCTTAACTGTTTTATTTGAAGGAAACCTGTGTGATTTAAAAAGTTAATAGCTTTTGCAAC TTTATTACTGGTTATATATATTTGGCCATTATAATGTGCAAGCAATTGGAAAAAAGTCAAGTAAATGCTT GTTTTTATAGTAGTTTGTTCTTGTTAAAATGTTCCTATGATAATGTCTGTAAACAGCACCGATTTGATTA CACTAGATGTAGTGTTGTAATAAACTGTTTATGGGGCTGATGTGTAAAGCTGTTCACGTTATTTGATGTT TACACCTCAGGGAAAGGCTTGTGTTCAGCAATATCTAAAGATAATGTTACTATGACAACATTTTTACTGT CCTTTAAAAAGCATTGCAATAGCGTGTTTGGATATGCATCAATCTAATCTTGCGTTCAGTGAATTAAACA TGACTAATTAAGTGTCTATTGCCCTTGATTTTGATATTAGAATAGGTGACTACATGGGTATTTAATATTT CTATATTCTGCTTTTCTAGCTGTTTTTACCTAGTTAGCTTGTGATTTTGCTGAATGGTATGTAAACGTGT AAAAACTGAAATTTGACAGAGCAATCCAGTCAATGTGTTAGGGGTCAAAAAAATTGAAGGCTGTAACATT TTTGGTAAAAACTCATGTATTTGCTACAGTAGTGCAGCTTAATTACAACCCAGATATACTGAAATAATAA ATTGTGAACTGCTAGCATTTAAGATAGATTTTGATAATTCTGGTTTCATGCAGTAATTTACTGTTTGATG CTGATGGTGCCATAGGCAGTGTAATTATGGCTATACAGCAAGATGCGTAAAGAAAGCTCTAGTATTGTAC TTGATTCAGATTTCTGAAGGGTGTGATGTTTATTAACATTGGGCTGTATTGTTCAGTGTGAATAATGAAA ATTTATAATTACACTGTCTTCATGGCTCAAGGAATTGTGTGTGTATTCCTGTAACCTTGTTGATCTATGC TTGATTAGAGTTAATCAGCTGATTTTTTTTTCTTTTTTTTTCCTTTCTTTCATTTTTTTTTTTAAAAGAT CCAGCCTTAATAAAAGGTTGTATTAAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Restriction Sites AscI-NotI |
ACCN NM_001013391
Insert Size 1656 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC068133, AAH68133
RefSeq Size 3012 bp
RefSeq ORF 1656 bp
Locus ID 432508
UniProt ID Q6NVF9
Cytogenetics 10 D2
Summary Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery. Plays a role in mRNA export.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
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"NM_001013391" in other vectors (4)
SKU Description Size Price
MG208746 Cpsf6 (tGFP-tagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6) 10 ug
$714.00
MR208746 Cpsf6 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6) 10 ug
$289.00 $514.00
MR208746L3 Lenti ORF clone of Cpsf6 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6) 10 ug
$814.00
MR208746L4 Lenti ORF clone of Cpsf6 (mGFP-tagged) - Mouse cleavage and polyadenylation specific factor 6 (Cpsf6) 10 ug
$814.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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