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Det1 (NM_029585) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208737
Det1 (Myc-DDK-tagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $513.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Det1
Synonyms 2610034H20Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208737 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCATCATGTTTCCACAATTAAACCTCGAAGAATCCAAAATCAAAATGTCATTCACCGCCTCGAAC
GCCGGCGCATCAGTTCAGGCAAGGCGGGCACCCACTGGCATCAGGTCCGAGTGTTCCACCAGAACGTCTT
CCCCAACTTCACGGTTGTCAACGTCGAAAAGCCTCCTTGTTTCTTACGGAAATTCTCGCCTGACGGGCGC
TACTTTATTGCCTTTTCATCTGACCAGACATCTCTTGAAATCTATGAGTACCAGGGCTGTCAGGCAGCAG
AGGACCTCTTGCAGGGATACGAAGGGGAGATCTTGTCCAATGGCAATGACCAGAGGTCGGTCAGCATCCG
AGGTCGGCTCTTTGAGCGTTTTTTTGTTCTGCTGCACATTACCAACGTGGCGGCCAATGGCGAGCACTTG
AACCGTGAGTGTAGCCTCTTCACAGATGACTGCCGCTGCGTCATCGTGGGCTCTGCTGCCTACCTCCCAG
ACGAGCCTCACCCTCCATTCTATGAGGTGTATCGGAACAGTGAGTCGGTGACCCCCAATCCACGGTCCCC
ACTTGAGGACTATTCCCTCCACATCATTGACCTTCACACTGGCCGTTTGTGTGACACACGCACATTCAAG
TGTGACAAAGTGGTCTTGTCGCACAATCAAGGGCTCTACTTGTATAAAAACATCCTGGCCATATTGTCTG
TGCAGCAGCAGACGATTCATGTCTTCCAGGTGACCCCTGAAGGCACCTTTATCGACGTTCGGACCATCGG
CCGCTTCTGCTACGAGGATGACCTGCTCACTGTGTCTGCCGTTTTCCCTGAGGTGCAGCGGGACAGCCAG
ACAGGCATGGCCAACCCTTTCAGGGACCCTTTCATCAACTCCTTGAAGCACCGCTTGCTGGTTTACCTCT
GGCGCCGGGCAGAGCAGGATGGCAGTGCCATGGCGAAGAGGCGGTTCTTCCAGTACTTTGACCAGCTGCG
CCAGCTGCGCATGTGGAAGATGCAGCTCCTGGACGAGAACCATCTGTTCATCAAGTACACCAGCGAGGAC
GTAGTGACGCTGCGGGTCACAGACCCGTCTCAGGCCTCCTTCTTTGTGGTGTACAACATGGTGACAACTG
AGGTGATTGCCGTGTTTGAGAACACATCCGATGAGCTTCTGGAACTCTTCGAGAACTTCTGTGACCTCTT
CCGCAACGCCACCCTGCACAGTGAAGTCCAGTTCCCTTGCTCAGCTTCTAGCAACAACTTTGCAAGGCAG
ATCCAGCGCCGGTTCAAAGACACGATTATAAATGCCAAGTATGGAGGGCACACGGAGGCTGTGCGCCGAC
TGCTAGGCCAGCTCCCCATCAGTGCCCAGTCTTACAGTGGGAGCCCATACCTGGATTTGTCTCTCTTCAG
CTACGATGACAAGTGGGTGTCAGTCATGGAGCGACCCAAGACTTGTGGAGATCACCCGATCAGGTTCTAT
GCCCGGGACTCTGGCTTGCTGAAGTTTGAGATCCAGGCGGGCTTGCTGGGCCGCCCCATCAACCACACAG
TGCGACGCCTCGTGGCCTTCACCTTTCACCCCTTTGAGCCATTTGCCATTTCTGTGCAGAGGACTAACGC
AGAGTATGTTGTCAACTTCCACATGCGACACTGCTGCACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208737 protein sequence
Red=Cloning site Green=Tags(s)

MDHHVSTIKPRRIQNQNVIHRLERRRISSGKAGTHWHQVRVFHQNVFPNFTVVNVEKPPCFLRKFSPDGR
YFIAFSSDQTSLEIYEYQGCQAAEDLLQGYEGEILSNGNDQRSVSIRGRLFERFFVLLHITNVAANGEHL
NRECSLFTDDCRCVIVGSAAYLPDEPHPPFYEVYRNSESVTPNPRSPLEDYSLHIIDLHTGRLCDTRTFK
CDKVVLSHNQGLYLYKNILAILSVQQQTIHVFQVTPEGTFIDVRTIGRFCYEDDLLTVSAVFPEVQRDSQ
TGMANPFRDPFINSLKHRLLVYLWRRAEQDGSAMAKRRFFQYFDQLRQLRMWKMQLLDENHLFIKYTSED
VVTLRVTDPSQASFFVVYNMVTTEVIAVFENTSDELLELFENFCDLFRNATLHSEVQFPCSASSNNFARQ
IQRRFKDTIINAKYGGHTEAVRRLLGQLPISAQSYSGSPYLDLSLFSYDDKWVSVMERPKTCGDHPIRFY
ARDSGLLKFEIQAGLLGRPINHTVRRLVAFTFHPFEPFAISVQRTNAEYVVNFHMRHCCT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029585
ORF Size 1650 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029585.2
RefSeq Size 2217 bp
RefSeq ORF 1653 bp
Locus ID 76375
UniProt ID Q9D0A0
Cytogenetics 7 D2
MW 63.8 kDa
Summary Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation (By similarity).UniProtKB/Swiss-Prot Function
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"NM_029585" in other vectors (4)
SKU Description Size Price
MC218781 Det1 (untagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1), (10ug) 10 ug
$564.00
MG208737 Det1 (tGFP-tagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1) 10 ug
$713.00
MR208737L3 Lenti ORF clone of Det1 (Myc-DDK-tagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1) 10 ug
$813.00
MR208737L4 Lenti ORF clone of Det1 (mGFP-tagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1) 10 ug
$813.00

Plasmid Map for MR208737

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208737
Circular map for MR208737

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