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Det1 (NM_029585) Mouse Untagged Clone

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SKU
MC218781
Det1 (untagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $564.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Det1
Synonyms 2610034H20Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC218781 representing NM_029585
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCATCATGTTTCCACAATTAAACCTCGAAGAATCCAAAATCAAAATGTCATTCACCGCCTCGAAC
GCCGGCGCATCAGTTCAGGCAAGGCGGGCACCCACTGGCATCAGGTCCGAGTGTTCCACCAGAACGTCTT
CCCCAACTTCACGGTTGTCAACGTCGAAAAGCCTCCTTGTTTCTTACGGAAATTCTCGCCTGACGGGCGC
TACTTTATTGCCTTTTCATCTGACCAGACATCTCTTGAAATCTATGAGTACCAGGGCTGTCAGGCAGCAG
AGGACCTCTTGCAGGGATACGAAGGGGAGATCTTGTCCAATGGCAATGACCAGAGGTCGGTCAGCATCCG
AGGTCGGCTCTTTGAGCGTTTTTTTGTTCTGCTGCACATTACCAACGTGGCGGCCAATGGCGAGCACTTG
AACCGGGAGTGTAGCCTCTTCACAGATGACTGCCGCTGCGTCATCGTGGGCTCTGCTGCCTACCTCCCAG
ACGAGCCTCACCCTCCATTCTATGAGGTGTATCGGAACAGTGAGTCGGTGACCCCCAATCCACGGTCCCC
ACTTGAGGACTATTCCCTCCACATCATTGACCTTCACACTGGCCGTTTGTGTGACACACGCACATTCAAG
TGTGACAAAGTGGTCTTGTCGCACAATCAAGGGCTCTACTTGTATAAAAACATCCTGGCCATATTGTCTG
TGCAGCAGCAGACGATTCATGTCTTCCAGGTGACCCCTGAAGGCACCTTTATCGACGTTCGAACCATCGG
CCGCTTCTGCTATGAGGATGACCTGCTCACTGTGTCTGCCGTTTTCCCTGAGGTGCAGCGGGACAGCCAG
ACAGGCATGGCCAACCCTTTCAGGGACCCTTTCATCAACTCCTTGAAGCACCGCTTGCTGGTTTACCTCT
GGCGCCGGGCAGAGCAGGATGGCAGTGCCATGGCGAAGAGGCGGTTCTTCCAGTACTTTGACCAGCTGCG
CCAGCTGCGCATGTGGAAGATGCAGCTCCTGGACGAGAACCATCTGTTCATCAAGTACACCAGCGAGGAC
GTAGTGACGCTGCGGGTCACAGACCCGTCTCAGGCCTCCTTCTTTGTGGTATACAACATGGTGACAACAG
AGGTGATTGCCGTGTTTGAGAACACATCCGATGAGCTTCTGGAACTCTTCGAGAACTTCTGTGACCTCTT
CCGCAACGCCACCCTGCACAGTGAAGTCCAATTCCCTTGCTCAGCTTCTAGCAACAACTTTGCAAGGCAG
ATCCAGCGCCGGTTCAAAGACACGATTATAAATGCCAAGTATGGAGGGCACACGGAGGCTGTGCGCCGAC
TGCTAGGCCAGCTCCCCATCAGTGCCCAGTCTTACAGTGGGAGCCCATACCTGGATTTGTCTCTCTTCAG
CTACGATGACAAGTGGGTGTCAGTCATGGAGCGACCCAAGACTTGTGGAGATCACCCGATCAGGTTCTAT
GCCCGGGACTCTGGCTTGCTGAAGTTTGAGATCCAGGCGGGCTTGCTGGGCCGCCCCATCAACCACACAG
TGCGACGCCTCGTGGCCTTCACCTTTCACCCCTTTGAGCCATTTGCCATTTCTGTGCAGAGGACTAACGC
AGAGTATGTTGTCAACTTCCACATGCGACACTGCTGCACCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_029585
Insert Size 1653 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029585.3, NP_083861.1
RefSeq Size 2217 bp
RefSeq ORF 1653 bp
Locus ID 76375
UniProt ID Q9D0A0
Cytogenetics 7 D2
Summary Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029585" in other vectors (4)
SKU Description Size Price
MG208737 Det1 (tGFP-tagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1) 10 ug
$713.00
MR208737 Det1 (Myc-DDK-tagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1) 10 ug
$289.00 $513.00
MR208737L3 Lenti ORF clone of Det1 (Myc-DDK-tagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1) 10 ug
$813.00
MR208737L4 Lenti ORF clone of Det1 (mGFP-tagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1) 10 ug
$813.00

file-alt Citations

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