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Siae (NM_011734) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208648
Siae (Myc-DDK-tagged) - Mouse sialic acid acetylesterase (Siae)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $505.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Siae or NM_011734"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Siae
Synonyms LSE; Ysg2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208648 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTTCCCCGGGGCCTGTGTTTGGGATAGTGCTGCTCATAATCGCGCGAGTCAGCAGAAGTGCAGGTA
TTGGTTTTCGCTTTGCTTCATACATCGATAATTACATGGTGCTGCAGAAGGAGCCTTCAGGGGCTGTGAT
CTGGGGCTTTGGTACACCTGGAGCCACAGTGACAGTGACCTTGTGCCAAGGTCAGGAAACCATCATGAAG
AAAGTGACCAGTGTGAAAGAACCCTCCAACACCTGGATGGTGGTACTGGACCCTATGAAGCCTGGGGGAC
CTTTTGAAGTGATGGCGCAACAGACTTTGGGGACAATGAATTTCACCCTGAGAGTCCATGATGTCTTATT
TGGAGATGTCTGGCTTTGCAGTGGGCAGAGTAACATGCAGATGACTGTTTCACAGATCTTTAACGCCTCA
AAGGAGTTGTCCGACACTGCTGCCTACCAGTCTGTGCGCATCTTCTCTGTCTCCCTCACCCAATCAGAGG
AGGAGTTGGATGACCTTACTGAGGTTGACCTGTCATGGTCCAAGCCCACCGCAGGAAACTTGGGCCATGG
AAATTTCACTTACATGTCTGCGGTGTGCTGGCTCTTTGGGCGTTACCTGTATGACACTCTGCAATATCCC
ATTGGGTTGGTCTCCTCCAGTTGGGGTGGGACGTACATTGAAGTCTGGTCATCTAGAAGGACACTGAAAG
CCTGTGGAGTCCCTAATACAAGGGATGAAAGAGTTGGTCAGCCCGAGATAAAGCCCATGAGGAATGAATG
TAACAGTGAGGAGTCCTCATGTCCTTTTAGGGTTGTCCCATCTGTTCGTGTGACTGGACCGACAAGACAC
TCTGTCCTTTGGAACGCCATGATCCATCCACTGCAAAACATGACTTTGAAAGGGGTGGTGTGGTACCAGG
GAGAGTCCAATGCGGATTATAACAGGGACCTGTACACCTGCATGTTTCCTGAACTCATTGAAGACTGGCG
CCAGACCTTCCACTACGGCTCCCAGGGTCAGACAGATCGTTTCTTTCCATTTGGGTTTGTCCAGCTGTCT
TCATACATGTTAAAGAACTCATCGGATTATGGATTTCCAGAGATCCGTTGGCATCAAACTGCAGACTTTG
GCCATGTCCCCAACCTGAAGATGCCTAATACTTTTATGGCTGTCGCTATTGACCTCTGTGATAGAGACTC
ACCCTTTGGAAGTATCCACCCTCGAGACAAACAGACTGTGGCCTATCGTCTGCACTTGGGTGCTCGAGCT
GTGGCATATGGTGAGAAAAATTTGACCTTTCAAGGACCACTACCTAAGAAGATAGAACTCTTGGCTTCCA
ATGGCCTGCTCAACCTCACATATGACCAGGAAATCCAGGTGCAAATGCAAGATAACAAAACATTTGAGAT
CTCCTGTTGTAGTGACCGTCATTGCAAATGGCTTCCAGCTCCTGTTAACACTTTCTCCACCCAGACTCTG
ATTTTGGATCTCAATGCTTGTCTTGGCACAGTGGTTGCTGTTCGCTATGCCTGGACCACATGGCCTTGTG
AATATAAGCAATGTGCTGTTTACCACACCAGCAGTATGTTGCCAGCTCCTCCCTTCATTGCTCAAATTTC
ACACAGGGGTATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208648 protein sequence
Red=Cloning site Green=Tags(s)

MVSPGPVFGIVLLIIARVSRSAGIGFRFASYIDNYMVLQKEPSGAVIWGFGTPGATVTVTLCQGQETIMK
KVTSVKEPSNTWMVVLDPMKPGGPFEVMAQQTLGTMNFTLRVHDVLFGDVWLCSGQSNMQMTVSQIFNAS
KELSDTAAYQSVRIFSVSLTQSEEELDDLTEVDLSWSKPTAGNLGHGNFTYMSAVCWLFGRYLYDTLQYP
IGLVSSSWGGTYIEVWSSRRTLKACGVPNTRDERVGQPEIKPMRNECNSEESSCPFRVVPSVRVTGPTRH
SVLWNAMIHPLQNMTLKGVVWYQGESNADYNRDLYTCMFPELIEDWRQTFHYGSQGQTDRFFPFGFVQLS
SYMLKNSSDYGFPEIRWHQTADFGHVPNLKMPNTFMAVAIDLCDRDSPFGSIHPRDKQTVAYRLHLGARA
VAYGEKNLTFQGPLPKKIELLASNGLLNLTYDQEIQVQMQDNKTFEISCCSDRHCKWLPAPVNTFSTQTL
ILDLNACLGTVVAVRYAWTTWPCEYKQCAVYHTSSMLPAPPFIAQISHRGI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011734
ORF Size 1623 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011734.1, NM_011734.2, NM_011734.3, NP_035864.2
RefSeq Size 3433 bp
RefSeq ORF 1626 bp
Locus ID 22619
UniProt ID P70665
Cytogenetics 9 20.79 cM
MW 60.8 kDa
Summary Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid.UniProtKB/Swiss-Prot Function
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"NM_011734" in other vectors (4)
SKU Description Size Price
MC200476 Siae (untagged) - Mouse sialic acid acetylesterase (Siae), (10ug) 10 ug
$505.00
MG208648 Siae (tGFP-tagged) - Mouse sialic acid acetylesterase (Siae) 10 ug
$705.00
MR208648L3 Lenti ORF clone of Siae (Myc-DDK-tagged) - Mouse sialic acid acetylesterase (Siae) 10 ug
$805.00
MR208648L4 Lenti ORF clone of Siae (mGFP-tagged) - Mouse sialic acid acetylesterase (Siae) 10 ug
$805.00

Plasmid Map for MR208648

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR208648
Circular map for MR208648

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