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Yes1 (NM_009535) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208634
Yes1 (Myc-DDK-tagged) - Mouse Yamaguchi sarcoma viral (v-yes) oncogene homolog 1 (Yes1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $504.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Yes1
Synonyms p61-Yes; Yes
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208634 representing NM_009535
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATAAAATACACACCGGAAAATCTTACAGAGC
CTGTAAGCCCAAGTGCCAGTCATTATGGAGTGGAACATGCTACAGTTGCCCCGACCTCTTCCACAAAGGG
AGCATCAGTTAATTTTAACAGTCTTTCCATGACACCCTTTGGAGGGTCCTCAGGGGTGACTCCTTTTGGA
GGAGCGTCTTCCTCATTCTCAGTGGTGTCAAGTTCATATCCTACAGGTTTAACAGGTGGTGTCACTATAT
TTGTGGCCTTGTATGATTATGAAGCTAGAACTACAGAAGACCTTTCCTTTAAGAAGGGTGAACGATTTCA
AATAATTAACAATACGGAAGGAGACTGGTGGGAAGCAAGATCAATTGCTACCGGAAAGAGTGGTTATATC
CCTAGCAATTACGTAGTGCCTGCAGATTCCATTCAGGCAGAAGAATGGTATTTTGGCAAAATGGGGAGAA
AAGATGCGGAAAGATTACTTCTGAATCCTGGGAATCAGCGAGGTATTTTCTTAGTAAGAGAAAGTGAAAC
TACTAAAGGTGCTTACTCCCTCTCAATCCGTGATTGGGATGAGGTGAGGGGTGACAATGTGAAGCATTAC
AAGATCAGAAAACTTGACAATGGTGGCTACTACATCACGACCAGAGCTCAGTTTGATACACTGCAGAAGC
TGGTGAAGCACTACACAGAACATGCTGATGGATTATGCCACAAGTTAACAACTGTGTGTCCTACTGTGAA
ACCCCAGACTCAAGGTCTGGCAAAAGATGCTTGGGAAATCCCTCGAGAATCATTGCGACTAGAGGTGAAA
CTAGGTCAAGGATGCTTTGGGGAAGTGTGGATGGGAACATGGAATGGAACTACAAAAGTAGCAATCAAAA
CACTAAAGCCAGGTACAATGATGCCAGAAGCATTCCTTCAAGAAGCTCAGATAATGAAAAAGCTAAGACA
CGATAAACTTGTTCCACTCTATGCAGTTGTTTCTGAAGAGCCCATTTATATTGTCACCGAGTTTATGTCA
AAAGGAAGCTTGTTAGATTTCCTTAAAGAAGGAGATGGAAAGTATTTGAAGCTTCCACAGCTGGTTGATA
TGGCTGCTCAGATCGCTGATGGCATGGCGTATATTGAAAGAATGAACTATATTCACCGAGATCTCCGAGC
TGCTAATATTCTTGTAGGAGAAAATCTTATATGCAAAATAGCAGATTTTGGCTTAGCAAGATTAATTGAA
GACAATGAATACACGGCAAGACAAGGTGCAAAATTTCCAATCAAGTGGACAGCTCCTGAGGCTGCTCTGT
ATGGTCGATTTACAATAAAGTCAGATGTGTGGTCATTTGGAATTCTACAGACAGAGCTGGTAACAAAAGG
AAGAGTGCCATATCCAGGTATGGTAAACCGTGAAGTATTGGAACAAGTAGAGCGGGGATACAGAATGCCT
TGCCCCCAGGGCTGTCCCGAATCCCTCCATGAATTGATGAATCTTTGCTGGAAGAAGGATCCTGATGAAA
GACCAACATTTGAATATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAGAGCCACAGTACCAACC
AGGAGAAAATTTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208634 representing NM_009535
Red=Cloning site Green=Tags(s)

MGCIKSKENKSPAIKYTPENLTEPVSPSASHYGVEHATVAPTSSTKGASVNFNSLSMTPFGGSSGVTPFG
GASSSFSVVSSSYPTGLTGGVTIFVALYDYEARTTEDLSFKKGERFQIINNTEGDWWEARSIATGKSGYI
PSNYVVPADSIQAEEWYFGKMGRKDAERLLLNPGNQRGIFLVRESETTKGAYSLSIRDWDEVRGDNVKHY
KIRKLDNGGYYITTRAQFDTLQKLVKHYTEHADGLCHKLTTVCPTVKPQTQGLAKDAWEIPRESLRLEVK
LGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMS
KGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLICKIADFGLARLIE
DNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMP
CPQGCPESLHELMNLCWKKDPDERPTFEYIQSFLEDYFTATEPQYQPGENL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009535
ORF Size 1623 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009535.3, NP_033561.1
RefSeq Size 4608 bp
RefSeq ORF 1626 bp
Locus ID 22612
UniProt ID Q04736, Q3TJI7
Cytogenetics 5 17.33 cM
MW 61.1 kDa
Summary Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity).UniProtKB/Swiss-Prot Function
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"NM_009535" in other vectors (4)
SKU Description Size Price
MC200981 Yes1 (untagged) - Mouse Yamaguchi sarcoma viral (v-yes) oncogene homolog 1 (Yes1), transcript variant 1, (10ug) 10 ug
$505.00
MG208634 Yes1 (tGFP-tagged) - Mouse Yamaguchi sarcoma viral (v-yes) oncogene homolog 1 (Yes1) 10 ug
$489.00 $704.00
MR208634L3 Lenti ORF clone of Yes1 (Myc-DDK-tagged) - Mouse Yamaguchi sarcoma viral (v-yes) oncogene homolog 1 (Yes1), transcript variant 1 10 ug
$804.00
MR208634L4 Lenti ORF clone of Yes1 (mGFP-tagged) - Mouse Yamaguchi sarcoma viral (v-yes) oncogene homolog 1 (Yes1), transcript variant 1 10 ug
$804.00

Plasmid Map for MR208634

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208634
Circular map for MR208634

file-alt Citations

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