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Kcnn1 (NM_032397) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208601
Kcnn1 (Myc-DDK-tagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1 (Kcnn1)
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $502.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kcnn1
Synonyms AI854248; KCa2.1; SK1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208601 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTAGCCACAGCCACAATGGCAGCGTGGGGCAGCCTCTGGGCAGCGGGCCTGGATTCCTGGGCTGGG
AGCCTGTGGACCCTGAGGCAGGCCGCCCCCTGCAGCCCACCCAAGGCCCAGGCCTGCAGATGGTGGCCAA
GGGTCAGCCTGTCAGGCTGTCACCCGGTGGTTCCAGGGGCCACCCCCAGGAGCAGGAGGAGGAAGAGGAA
GAGGAGGAGGAGGAGGAGGACAAGACAGGCTCAGGGAAGCCCCCAACAGTCAGCCACCGCCTGGGACACC
GCAGGGCCCTCTTTGAAAAGCGTAAACGGCTCAGTGACTATGCGCTCATCTTTGGCATGTTTGGGATTGT
CGTCATGGTGACAGAAACAGAGCTGTCCTGGGGTGTATACACCAAGGAGTCACTCTGCTCTTTTGCTCTG
AAATGCCTCATCAGCCTGTCCACTGTCATCTTGCTTGGCCTTGTCATCCTGTACCACGCCCGAGAGATCC
AGCTGTTCTTGGTGGACAATGGTGCCGACGACTGGCGTATCGCCATGACGTGGGAGCGCGTGTCCCTGAT
CTCGCTGGAGTTGGTCGTGTGTGCCATCCACCCGGTGCCCGGCCACTATCGCTTCACGTGGACGGCACGA
CTGGCCTTCTCTCTGGTGCCGTCGGCAGCCGAGGCAGACCTGGATGTGCTGCTGTCCATCCCCATGTTCC
TGCGCCTCTACCTGCTGGCTCGGGTCATGCTCCTGCACAGCCGCATCTTCACCGATGCATCCAGCCGCAG
CATCGGGGCCCTTAACCGCGTCACCTTCAACACACGCTTCGTCACCAAGACGCTCATGACCATCTGCCCC
GGCACTGTGTTGTTGGTCTTCAGCGTCTCCTCCTGGATCGTTGCTGCGTGGACAGTGCGCGTGTGTGAGA
GGTACCACGATAAGCAGGAAGTGACCAGCAACTTCCTGGGAGCCATGTGGCTCATCTCCATCACCTTCTT
GTCCATTGGCTATGGAGACATGGTGCCGCATACCTACTGTGGGAAGGGTGTGTGTCTGCTCACTGGCATC
ATGGGAGCAGGCTGCACTGCACTCGTGGTGGCCGTCGTGGCTCGGAAGTTGGAACTCACCAAGGCTGAGA
AACACGTGCACAACTTCATGATGGACACACAGCTCACCAAGCGGGTCAAAAATGCTGCTGCAAACGTTCT
CAGGGAGACATGGCTCATCTACAAACACACCAGGCTGGTGAAGAAGCCAGACCAAGGCCGGGTTCGGAAA
CACCAGCGTAAGTTCCTTCAGGCCATCCATCAGGCTCAGAAGCTCCGAAGTGTGAAGATTGAACAAGGGA
AGGTGAACGATCAGGCCAACACGCTGGCTGAGCTGGCCAAGGCACAGAGCATCGCATATGAGGTGGTGTC
AGAGCTGCAGGCCCAGCAGGAGGAGTTGGAGGCACGCCTAGCCGCCTTGGAGAGCCGACTGGATGTCCTG
GGTGCCTCCCTGCAGGCTCTACCAGGCCTTATAGCCCAAGCCATATGCCCTCTACCACCACCCTGGCCTG
GGCCTGGTCACCTGGCCACAGCCACCCAGAGCCCACAAAGCCACTGGCTGCCCACCATGGGATCAGACTG
TGGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208601 protein sequence
Red=Cloning site Green=Tags(s)

MSSHSHNGSVGQPLGSGPGFLGWEPVDPEAGRPLQPTQGPGLQMVAKGQPVRLSPGGSRGHPQEQEEEEE
EEEEEEDKTGSGKPPTVSHRLGHRRALFEKRKRLSDYALIFGMFGIVVMVTETELSWGVYTKESLCSFAL
KCLISLSTVILLGLVILYHAREIQLFLVDNGADDWRIAMTWERVSLISLELVVCAIHPVPGHYRFTWTAR
LAFSLVPSAAEADLDVLLSIPMFLRLYLLARVMLLHSRIFTDASSRSIGALNRVTFNTRFVTKTLMTICP
GTVLLVFSVSSWIVAAWTVRVCERYHDKQEVTSNFLGAMWLISITFLSIGYGDMVPHTYCGKGVCLLTGI
MGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKHTRLVKKPDQGRVRK
HQRKFLQAIHQAQKLRSVKIEQGKVNDQANTLAELAKAQSIAYEVVSELQAQQEELEARLAALESRLDVL
GASLQALPGLIAQAICPLPPPWPGPGHLATATQSPQSHWLPTMGSDCG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032397
ORF Size 1614 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032397.3
RefSeq Size 4042 bp
RefSeq ORF 1617 bp
Locus ID 84036
UniProt ID Q9EQR3
Cytogenetics 8 B3.3
MW 59.4 kDa
Summary Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_032397" in other vectors (3)
SKU Description Size Price
MC217935 Kcnn1 (untagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1 (Kcnn1), (10ug) 10 ug
$552.00
MR208601L3 Lenti ORF clone of Kcnn1 (Myc-DDK-tagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1 (Kcnn1) 10 ug
$802.00
MR208601L4 Lenti ORF clone of Kcnn1 (mGFP-tagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1 (Kcnn1) 10 ug
$802.00

Plasmid Map for MR208601

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208601
Circular map for MR208601

file-alt Citations

Neuronal overexpression of Kcnn1 in A53T α-synuclein mice suppresses phospho-serine 129 α-synuclein formation and doubles survival time
Nagy, Fenton, Horwich
Proc Natl Acad Sci U S A (2026) 123 (34), e2622262123 DOI: 10.1073/pnas.2622262123
AI Summary: OriGene’s mouse Kcnn1 cDNA plasmid (MR208601) was used as the template to generate the Kcnn1 overexpression construct, enabling the study of its neuroprotective effects in the A53T α-synuclein mouse model.
Snippet: n1 cDNA. The mouse Kcnn1 cDNA sequence (NM_032397), was PCR-amplified from OriGene Technologies plasmid MR208601, omitting the 3′-terminal Myc-DDK tag sequence. The final construct was sequenced after preparation. This plasmid was packaged at the University of North Caroli
Neuronal overexpression of mouse potassium channel subunit Kcnn1 in A53T α-synuclein mice more than doubles median survival time, associated with suppression of phospho-S129 α-synuclein formation
Nagy, Fenton, Horwich 
bioRxiv (2026)
AI Summary: OriGene’s mouse Kcnn1 cDNA plasmid (catalog MR208601) was used as the template to generate the Kcnn1 overexpression construct employed in the study.
Snippet: modified by direct replacement of the GFP sequence with one encoding mouse Kcnn1 cDNA. The mouse Kcnn1 cDNA sequence (NM_032397), was PCR-amplified from OriGene Technologies plasmid MR208601, omitting the 3’-terminal Myc-DDK tag sequence. The final construct was sequenced after preparation. This plasmid was package
Showing 1-2 of 2 papers.
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