OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Cnot2 (BC063105) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR208509
Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $494.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Cnot2 or BC063105"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cnot2
Synonyms 2600016M12Rik; 2810470K03Rik; AA537049; AA959607; AW557563; C79650
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208509 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAAAGAAGTTGCACAGGTGACAAACAGCATGTTTGGTGCTTCAAGAAAGAAGTTTGTAGAGGGGG
TGGACAGCGACTACCATGATGAGAACATGTACTACAGCCAGTCTTCTATGTTCCCACATCGGTCAGAGAA
AGATATGCTGGCATCGCCATCTACGTCAGGTCAGCTGTCTCAATTTGGGGCAAGTTTATACGGGCAACAA
AGTGCACTAGGCCTTCCAATGAGGGGGATGAGCAACAATACCCCTCAGTTAAATCGCAGCTTATCACAAG
GCACTCAGTTACCGAGCCACGTCACGCCAACAACAGGGGTACCAACAATGTCACTTCACACGCCTCCATC
TCCAAGCAGGGGTATTTTGCCTATGAATCCTAGGAATATGATGAACCACTCCCAGGTTGGTCAGGGCATT
GGAATTCCTAGCAGGACAAATAGCATGAGCAGTTCAGGGTTAGGTAGCCCCAACAGAAGCTCGCCAAGCA
TAATATGTATGCCAAAGCAACAGCCTTCTCGACAGCCTTTTACTGTGAACAGTATGTCTGGATTTGGAAT
GAACAGGAATCAGGCATTTGGAATGAATAACTCCTTATCAAGTAACATTTTTAATGGAACAGATGGCAGT
GAAAATGTGACAGGATTGGACCTTTCAGATTTCCCAGCGTTAGCTGACCGGAATAGAAGGGAAGGGAGCG
GCAACCCAACTCCATTAATAAACCCCTTGGCTGGAAGAGCTCCTTACGTTGGAATGGTAACAAAACCTGC
AAATGAGCAATCCCAAGACTTCTCAATACACAACGAAGATTTTCCAGCATTACCTGGTTCCAGCTATAAA
GATCCAACGTCAAGTAATGACGACAGCAAATCTAATTTGAGTACATCGGGGAAGACGACTTCAAGTACAG
ATGGACCCAAATTCCCTGGAGATAAAAGTTCAACAACACAAAACAATAATCAACAGAAAAAGGGGATCCA
GGTGTTACCTGATGGTCGAGTTACTAACATTCCTCAAGGGATGGTGACGGACCAATTTGGAATGATTGGC
CTGTTAACATTTATCAGGGCAGCAGAGACAGACCCAGGAATGGTACATCTTGCTTTAGGAAGTGACTTAA
CAACATTAGGCCTCAATCTGAACTCACCTGAAAATCTCTATCCCAAATTTGCATCACCCTGGGCTTCTTC
ACCTTGTCGGCCTCAAGACATAGACTTCCATGTTCCATCAGAATATTTAACAAACATTCACATTAGGGAT
AAGCTGGCTGCAATCAAACTTGGCCGATATGGAGAAGACCTCCTCTTCTATCTGTATTACATGAACGGCG
GGGATGTATTACAACTCTTAGCTGCGGTAGAACTTTTTAACCGTGATTGGAGATACCACAAGGAGGAGCG
GGTATGGATTACCAGGGCACCAGGCATGGAGCCAACAATGAAAACCAACACGTATGAGCGGGGGACCTAC
TATTTCTTTGACTGTCTCAACTGGAGGAAAGTAGCTAAGGAGTTCCATCTGGAATATGACAAATTAGAAG
AGCGGCCTCACCTGCCATCCACCTTCAACTACAACCCTGCTCAGCAAGCCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208509 protein sequence
Red=Cloning site Green=Tags(s)

MLKEVAQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYGQQ
SALGLPMRGMSNNTPQLNRSLSQGTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMMNHSQVGQGI
GIPSRTNSMSSSGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSSNIFNGTDGS
ENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIHNEDFPALPGSSYK
DPTSSNDDSKSNLSTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKGIQVLPDGRVTNIPQGMVTDQFGMIG
LLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSEYLTNIHIRD
KLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTYERGTY
YFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC063105
ORF Size 1593 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC063105, AAH63105
RefSeq Size 3014 bp
RefSeq ORF 1595 bp
Locus ID 72068
Cytogenetics 10 D2
MW 58.6 kDa
Summary Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"BC063105" in other vectors (4)
SKU Description Size Price
MC217882 Cnot2 (untagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026), (10ug) 10 ug
$545.00
MG208509 Cnot2 (tGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026) 10 ug
$694.00
MR208509L3 Lenti ORF clone of Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026) 10 ug
$794.00
MR208509L4 Lenti ORF clone of Cnot2 (mGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026) 10 ug
$794.00

Plasmid Map for MR208509

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208509
Circular map for MR208509

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products