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Nectin2 (NM_008990) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208504
Pvrl2 (Myc-DDK-tagged) - Mouse poliovirus receptor-related 2 (Pvrl2), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $741.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nectin2
Synonyms AI325026; AI987993; Cd112; MPH; nectin-2; Pvr; Pvrl2; Pvs
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208504 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGGGCCGCAGTCCTCCCGCCGTCCAGATTGTCACCGACGCTGCCGTTGTTGCCGCTGCTACTGC
TCCTGCTTCAGGAAACAGGAGCCCAAGATGTGCGGGTACGAGTGCTTCCCGAGGTCCGGGGCCGCTTGGG
AGGCACCGTGGAGTTACCGTGCCACCTGCTCCCACCCACGACGGAGCGCGTCTCTCAGGTGACCTGGCAG
CGCCTGGATGGCACAGTTGTGGCTGCTTTCCACCCATCCTTCGGAGTGGATTTCCCCAACTCTCAGTTCA
GCAAGGACCGTCTGTCCTTTGTCAGAGCGAGACCAGAAACAAACGCAGACCTGCGGGATGCCACACTGGC
CTTCCGGGGACTGAGGGTAGAGGACGAGGGCAATTACACCTGCGAGTTTGCCACGTTTCCCAACGGTACC
CGCAGGGGGGTGACCTGGCTCAGAGTCATAGCCCAGCCTGAGAACCACGCTGAAGCCCAGGAGGTCACAA
TTGGCCCCCAGTCGGTGGCTGTAGCCCGCTGTGTCTCCACTGGGGGCCGCCCCCCTGCCCGAATCACCTG
GATCTCATCTCTGGGTGGAGAGGCCAAAGATACTCAGGAGCCAGGGATACAGGCTGGCACCGTCACTATC
ATCAGCCGATACTCCTTGGTGCCCGTGGGCCGAGCGGATGGCGTCAAGGTCACGTGTAGAGTGGAACACG
AGAGCTTCGAAGAGCCGATCCTGCTGCCAGTGACCCTCTCTGTGCGCTACCCTCCAGAAGTATCCATCTC
CGGCTATGATGACAACTGGTACCTTGGCCGCAGTGAGGCCATACTGACCTGTGATGTACGAAGCAACCCA
GAGCCCACAGACTATGACTGGAGCACGACCTCGGGCGTCTTCCCAGCCTCTGCAGTGGCCCAGGGCTCTC
AGCTGCTTGTCCACTCTGTGGATCGAATGGTCAACACTACCTTCATCTGTACAGCCACCAACGCTGTGGG
GACAGGCCGTGCTGAGCAGGTCATCCTGGTGCGAGAGTCACCCAGCACAGCAGGAGCAGGGGCCACTGGT
GGCATCATTGGAGGTATTATCGCTGCCATCATCGCCACCGCAGTGGCTGGCACAGGCATCCTCATCTGCC
GACAACAGCGGAAGGAGCAGAGGCTTCAAGCTGCGGATGAGGAAGAAGAACTGGAAGGACCTCCCTCCTA
TAAACCACCCACCCCGAAGGCCAAGCTGGAGGAACCAGAGATGCCCTCTCAACTCTTCACCTTGGGGGCC
TCAGAGCACAGCCCAGTGAAGACGCCATACTTTGATGCTGGTGTCTCTTGTGCTGATCAGGAGATGCCTC
GGTATCACGAGCTGCCCACTCTGGAAGAGTGGTCAGGGCCCCTGCTGTTGGGGGCTACAGGCCTGGGACC
TTCTCTTCTGGTGCCTCCAGGACCCAATGTTGTGGAGGGGGTTTCCCTGAGTCTCGAAGATGAGGAGGAA
GATGATGAGGAGGAAGACTTCCTGGATAAAATCAACCCTATTTATGATGCCCTGTCCTACCCCAGCCCCT
CTGACTCCTACCAGAGCAAAGACTTTTTTGTGTCACGGGCCATGTATGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208504 protein sequence
Red=Cloning site Green=Tags(s)

MARAAVLPPSRLSPTLPLLPLLLLLLQETGAQDVRVRVLPEVRGRLGGTVELPCHLLPPTTERVSQVTWQ
RLDGTVVAAFHPSFGVDFPNSQFSKDRLSFVRARPETNADLRDATLAFRGLRVEDEGNYTCEFATFPNGT
RRGVTWLRVIAQPENHAEAQEVTIGPQSVAVARCVSTGGRPPARITWISSLGGEAKDTQEPGIQAGTVTI
ISRYSLVPVGRADGVKVTCRVEHESFEEPILLPVTLSVRYPPEVSISGYDDNWYLGRSEAILTCDVRSNP
EPTDYDWSTTSGVFPASAVAQGSQLLVHSVDRMVNTTFICTATNAVGTGRAEQVILVRESPSTAGAGATG
GIIGGIIAAIIATAVAGTGILICRQQRKEQRLQAADEEEELEGPPSYKPPTPKAKLEEPEMPSQLFTLGA
SEHSPVKTPYFDAGVSCADQEMPRYHELPTLEEWSGPLLLGATGLGPSLLVPPGPNVVEGVSLSLEDEEE
DDEEEDFLDKINPIYDALSYPSPSDSYQSKDFFVSRAMYV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008990
ORF Size 1590 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008990.2, NP_033016.2
RefSeq Size 2735 bp
RefSeq ORF 1593 bp
Locus ID 19294
UniProt ID P32507
Cytogenetics 7 9.94 cM
MW 57.3 kDa
Summary Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008990" in other vectors (3)
SKU Description Size Price
MG208504 Pvrl2 (tGFP-tagged) - Mouse poliovirus receptor-related 2 (Pvrl2) 10 ug
$941.00
MR208504L3 Lenti ORF clone of Pvrl2 (Myc-DDK-tagged) - Mouse poliovirus receptor-related 2 (Pvrl2), transcript variant 1 10 ug
$1,041.00
MR208504L4 Lenti ORF clone of Pvrl2 (mGFP-tagged) - Mouse poliovirus receptor-related 2 (Pvrl2), transcript variant 1 10 ug
$1,041.00

Plasmid Map for MR208504

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208504
Circular map for MR208504

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