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Efcab14 (NM_172698) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208481
Efcab14 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4732418C07 gene (4732418C07Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $493.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Efcab14
Synonyms 4732418C07Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208481 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAAAGCGCAAAGAGCTCAATGCGTTGATCGGCCTGGCTGGTGACCATCGGAGAAAGAAGACCAAGC
AAGGCTCAGGCAGCCACCGCCTGCTTCGCACTGAGCCTCCTGACTCAGACTCAGAGTCCAGCACAGACGA
AGAGGAATTTGGAGCAATTGGAAATCGCTCTCGTTTTGTCAAGGGAGACTATGCCCGATGCTGCAAGATC
TGCTGTCCCCTCTGTGCCTTTGTCATCCTCGCAGCCTGCGTTGTGGCCTCTGTGGGCTTGGTGTGGATGC
AAATGGCTCTCAAGGAGGATCTGGATGTCCTCAAGGAAAAATTTAGAACAATGGAGTCTAATCAGAAGAG
CTCATTCCAGGAAATCCCCAAACTTAATGAGGAACTTCTCAGCAAACAAAAACAACTTGAGAAGATTGAG
TCTGGGGAGCTGGGCTTAAGCAGAGTCTGGATCAACATCACAGAAATGAATAAGCAGATTTCTCTCTTGA
GTTCTGCAGTAAACCACCTAAAAGCCAGCGTCAAGTCAGCCGCAGACTTACTTAGTCTGCCTAGCACTGT
AGAAGGACTTCAGAAGAGTGTGGCTTCCATTGGCAATACTTTAAACAGTGTCCACCTTGCTGTAGAGGTG
ATACAGAAGACTGTGGATGAGCACAGGACAACCTTGGGGTTACTGCAGGGCAGTATGGAGAACAATGGAA
GCAACCAGATCTTGCCATCACCTTCACCTCCATCAGAGCTTGACAATAAATCCCACAGTGAAAGTGCAAA
ACAGGATATCCTGTACCTGCACAACTCCTTAGAGGAAGTCAACAGTACTGTAGTGGAATACCAGAGACAG
AATGACCTTAAACTCAAGGGAATGAGCGAGACCCTCAGTAACCTGACACAGAGACTCAGCCTGATAGAAA
GCCATGTGGTTGCACTGAGCAAGGCAGAACAGAGAACAAACGTGAGCTCCAGCACGATGGAGAATAGGGC
TGCCACTCTGAAAAGAGAGTCTTTGGTGACCAACAGAAGTGACACTGTACAGGCGCAAAGCATGAAGAAA
GAAGATAATTCAAATTCTCAGGTATCCGAGCTTCGAGAAAAACTGCAGCTGATCAGTGCTCTCACAAACA
AACCTGAAAGCAACAGGCCTCCGGAGACCACAGATGAAGAGCAAGTACAGAACTTCACATCAGATCCTTC
AGCATTGCCAGAATTTTCACAGCTTCTTAGAAACCAAATTGAGACCCAAGTAAAACCTCTCTCCCTGCCT
GGGATTTCTAGCATCAAAGATCTTCAGGATTTATTCCACAAGACTGGTCAAGATGTGGATGGGATGCTGA
CCTACCAGGAACTCTGGAACTCCCTAGGTTCTGCCATGCCAAGACCAGAGAGCTTGAGAGCATTTGATTC
GAATGGAGATGGAAGATACTCTTTCCTGGAACTAAGATTAGCTTTAGGATGCCTAAAGCCAAAATGTGGT
CTGATGAAACAGGAAATCATATTCTTTCTTCCTAATTGTTCACTCAAAAGGAGCTATGGGTTTTCTGTCC
AGAGGATACCACATGGAGCAGGCGTGCTTGGAGCAGGAGCCTTCTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208481 protein sequence
Red=Cloning site Green=Tags(s)

MKKRKELNALIGLAGDHRRKKTKQGSGSHRLLRTEPPDSDSESSTDEEEFGAIGNRSRFVKGDYARCCKI
CCPLCAFVILAACVVASVGLVWMQMALKEDLDVLKEKFRTMESNQKSSFQEIPKLNEELLSKQKQLEKIE
SGELGLSRVWINITEMNKQISLLSSAVNHLKASVKSAADLLSLPSTVEGLQKSVASIGNTLNSVHLAVEV
IQKTVDEHRTTLGLLQGSMENNGSNQILPSPSPPSELDNKSHSESAKQDILYLHNSLEEVNSTVVEYQRQ
NDLKLKGMSETLSNLTQRLSLIESHVVALSKAEQRTNVSSSTMENRAATLKRESLVTNRSDTVQAQSMKK
EDNSNSQVSELREKLQLISALTNKPESNRPPETTDEEQVQNFTSDPSALPEFSQLLRNQIETQVKPLSLP
GISSIKDLQDLFHKTGQDVDGMLTYQELWNSLGSAMPRPESLRAFDSNGDGRYSFLELRLALGCLKPKCG
LMKQEIIFFLPNCSLKRSYGFSVQRIPHGAGVLGAGAFW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172698
ORF Size 1587 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172698.2, NP_766286.2
RefSeq Size 4320 bp
RefSeq ORF 1590 bp
Locus ID 230648
Cytogenetics 4 D1
MW 58.6 kDa
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"NM_172698" in other vectors (3)
SKU Description Size Price
MG208481 Efcab14 (tGFP-tagged) - Mouse RIKEN cDNA 4732418C07 gene (4732418C07Rik) 10 ug
$693.00
MR208481L3 Lenti ORF clone of Efcab14 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4732418C07 gene (4732418C07Rik) 10 ug
$793.00
MR208481L4 Lenti ORF clone of Efcab14 (mGFP-tagged) - Mouse RIKEN cDNA 4732418C07 gene (4732418C07Rik) 10 ug
$793.00

Plasmid Map for MR208481

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208481
Circular map for MR208481

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