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Alg8 (NM_199035) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208441
Alg8 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase) (Alg8)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $490.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Alg8 or NM_199035"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Alg8
Synonyms AI447372; Gm1089
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208441 representing NM_199035
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGTCCGGGTCTGCAACCGCTGGTGGCCATTGGTTCTCTGCTTTGGCTCTTGGGGTGACTCTCC
TTAAGTGCCTTCTCATCCCCACCTACCATTCCACGGATTTTGAAGTACACCGAAATTGGCTTGCAATCAC
TCACAGCCTCCCAATATCTCAGTGGTATTATGAGGCAACTTCAGAGTGGACCTTGGATTACCCCCCCTTT
TTTGCATGGTTTGAGTACGCCTTATCACATATTGCCAAATACTTTGATCAAGAGATGCTCAATATTCATA
ACCTGAACTATTACAGTTCAAGGACATTGCTTTTCCAGAGGTTTTCTGTCATCTTGACAGATGCACTCTT
TGTGTATGCTGTCCATGAGTGCTGTAAATGCATTGATGGAAAAAGAACAGGCAAAGACCTCACAGAGAAA
CCAAAGTTTATTCTGTCCGTGCTCCTGCTGTGGAACTTTGGGTTGTTAATTGTAGACCATATTCATTTTC
AGTACAATGGCTTTCTGTCTGGATTACTGCTGTTGTCCATTGCACGTCTATTTCAGAAAAGGCACATTGA
AGGGGCGTTGCTCTTTGCTGTCCTCCTCCATCTCAAGCACATCTACCTATATGTGGCACCAGCCTATGGT
GTCTATCTGCTGCGGTCCTACTGCTTCACTGCAAGTAAACCAGATGGCAGTGTCCGTTGGAGCAGCTTCA
GCGTTGTCCGCGTTACTTCCCTGGGGCTGATCGTGTTCCTAGTTTCTGCTCTTTCGTTGGGCCCTTTCCT
AGCCTTGAACCAGCTGCCACAAGTCTTTTCCCGACTCTTTCCTTTCAAGAGGGGCCTTTGCCATGCCTAC
TGGGCTCCAAACTTCTGGGCTTTGTACAATGCTTTGGACAAAGTACTGTCTGTAATTGGTTTGAAATTGA
AGCTTCTTGACCCCAGCCAGATCCCCAGGGCCTCAATGACAAGTGGTTTGGTTCAGCAGTTCCAGCACAC
AGTCCTTCCCTCAGTTTCCCCTTTGGCAACCCTCATCTGCACACTGATAGCCATATTGCCCTCTGTTTTC
TGTCTTTGGTTTAAACCCCAAGGGCCCAGAGGCTTTCTCCGATGCCTGGTTCTTTGTGCCTTGAGTTCCT
TCATGTTTGGGTGGCATGTCCATGAGAAAGCCATACTCCTTGCGATTCTCCCAATGAGCCTGTTGTCTGT
GGAAAAAGCAGGAGATGCGACAGTTTTTCTGATTCTGGCGACAACAGGACACTATTCCCTCTTCCCCCTG
CTCTTCACTGCACCAGAACTTCCCATTAAAATCTTACTCATGTTACTGTTCACGGTTTATAGTATTTCCT
CACTGAAGACACTATTCAGAAAAGAAAAACCTCTTTTTAATTGGATGGAAACTGTGTACCTCCTTGGCCT
GGGGCCTCTGGAAGTCTGCTGTGAATTCCTACTCCCGTTTACCTCCTGGAAGCTGAAGTACCCCTTCATC
CCTTTGCTGCTGACCTCGGTGTACTGTGCAGTGGGCATCACATACGCCTGGACCAGGCTGTATGCTTCAG
TGTTGACTGGCTCCCTTGTCAGCAAGACAAAGAAACAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208441 representing NM_199035
Red=Cloning site Green=Tags(s)

MAASGSATAGGHWFSALALGVTLLKCLLIPTYHSTDFEVHRNWLAITHSLPISQWYYEATSEWTLDYPPF
FAWFEYALSHIAKYFDQEMLNIHNLNYYSSRTLLFQRFSVILTDALFVYAVHECCKCIDGKRTGKDLTEK
PKFILSVLLLWNFGLLIVDHIHFQYNGFLSGLLLLSIARLFQKRHIEGALLFAVLLHLKHIYLYVAPAYG
VYLLRSYCFTASKPDGSVRWSSFSVVRVTSLGLIVFLVSALSLGPFLALNQLPQVFSRLFPFKRGLCHAY
WAPNFWALYNALDKVLSVIGLKLKLLDPSQIPRASMTSGLVQQFQHTVLPSVSPLATLICTLIAILPSVF
CLWFKPQGPRGFLRCLVLCALSSFMFGWHVHEKAILLAILPMSLLSVEKAGDATVFLILATTGHYSLFPL
LFTAPELPIKILLMLLFTVYSISSLKTLFRKEKPLFNWMETVYLLGLGPLEVCCEFLLPFTSWKLKYPFI
PLLLTSVYCAVGITYAWTRLYASVLTGSLVSKTKKH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199035
ORF Size 1578 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199035.2, NP_950200.2
RefSeq Size 2002 bp
RefSeq ORF 1581 bp
Locus ID 381903
UniProt ID Q6P8H8
Cytogenetics 7 E1
MW 60 kDa
Summary Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(1)Man(9)GlcNAc(2)-PP-Dol before it is transferred to the nascent peptide (By similarity). Required for PKD1/Polycystin-1 maturation and localization to the plasma membrane of the primary cilia (PubMed:28375157).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_199035" in other vectors (4)
SKU Description Size Price
MC217538 Alg8 (untagged) - Mouse asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase) (Alg8), (10ug) 10 ug
$540.00
MG208441 Alg8 (tGFP-tagged) - Mouse asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase) (Alg8) 10 ug
$690.00
MR208441L3 Lenti ORF clone of Alg8 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase) (Alg8) 10 ug
$790.00
MR208441L4 Lenti ORF clone of Alg8 (mGFP-tagged) - Mouse asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase) (Alg8) 10 ug
$790.00

Plasmid Map for MR208441

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208441
Circular map for MR208441

file-alt Citations

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