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Ncf2 (NM_010877) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208432
Ncf2 (Myc-DDK-tagged) - Mouse neutrophil cytosolic factor 2 (Ncf2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $490.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ncf2
Synonyms Ncf-2; NOXA2; p67phox
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208432 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCTGGCTGAGGCCATCAGACTCTGGAATGAAGGGGTGCTCGCAGCCGACAAGAAGGACTGGAAGG
GGGCCCTGGAGGCCTTCAGCGAGGTGCAGGACCCCCACTCGAGGATTTGCTTCAACATAGGCTGCGTGAA
CACCATCCTGGAAAACTTGCAGGCAGCCGAGCAGGCCTTCACCAAAAGCATCAACAGAGACAAGCACTCT
GCAGTGGCCTACTTCCAGAGAGGAATGCTCTACTACAGAATGGAGAAGTACGACCTTGCTATCAAAGACC
TTAAAGAGGCCTTGACGCAGCTTCGTGGGAACCAGCTGATAGACTACAAGATCCTGGGGCTGCAGTTCAA
GCTGTTTGCCTGTGAGGTATTGTACAATATTGCTCTCATGCATGCCAAGAAAGAGGAATGGAAGAAAGCA
GAAGAGCAGTTGGCATTGGCAACCAACATGAAGTCCGAGCCCAGGCATTCCAAGATCGACAAGGCCATGG
AGAGCATCTGGAAGCAGAAGCTGTTCGAGCCCGTGGTGATCCCTGTGGGTCGGCTGTTCCGTCCAAATGA
GAGGCAGGTGGCTCAGCTGGCCAAAAAGGACTATCTGGGCAAGGCTACGGTTGTAGCATCTGTGGTTCAC
CAAGACAACTTTTCTGGCTTCGCCCCTCTGCAGCCGCAGTCAGCAGAGCCTCCTCCCAGACCCAAAACCC
CAGAAATCTTCAGGGCTCTGGAAGGTGAGGCACACCGCGTATTGTTTGGCTTTGTGCCGGAGACGCCAGA
AGAGCTACAGGTCATGCCTGGGAACATCGTCTTTGTCTTGAAGAAGGGCAGTGATAACTGGGCCACAGTC
ATGTTCAATGGACAGAAGGGGCTTGTCCCCTGCAACTACCTGGAGCCAGTTGAGCTTCGGATTCACCCTC
AGTCGCAGCCCCAGGAAGATACCTCTCCAGAATCTGATATTCCACCACCTCCTAATTCTAGTCCCCCAGG
AAGACTCCAGTTGTCACCAGGTCACAAGCAAAAAGAGCCCAAGGAACTGAAGCTCAGCGTGCCTATGCCT
TACATGCTCAAGGTGCATTACAAATACACAGTGGTCATGGAGACGCGGCTTGGCCTCCCCTACAGCCAGC
TTCGGAACATGGTGTCTAAGAAGCTGGCGCTCTCGCCAGAACACACTAAACTGAGCTACCGGCGTCGGGA
CAGCCACGAGCTTCTGCTCCTGTCCGAAGAAAGCATGAAGGATGCCTGGGGCCAAGTGAAAAACTACTGC
CTGACTCTGTGGTGTGAGCATACGGTGGGTGACCAAGGTCTTATTGATGAACCCATACAAAGGGAAAACT
CAGACGCCAGTAAGCAGACTACGGAGCCTCAGCCTAAGGAGGGGACCCAGGTGGTAGCAATCTTCAGTTA
TGAGGCTGCCCAGCCAGAAGACCTGGAATTTGTGGAAGGAGATGTAATCCTGGTACTGTCACATGTGAAT
GAAGAATGGCTGGAAGGGGAGTGTAAAGGGAAAGTTGGCATTTTCCCGAAGGCTTTTGTTGAAGGATGTG
CAGCCAAGAATTTGGAAGGCATTCCCAGAGAAGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208432 protein sequence
Red=Cloning site Green=Tags(s)

MSLAEAIRLWNEGVLAADKKDWKGALEAFSEVQDPHSRICFNIGCVNTILENLQAAEQAFTKSINRDKHS
AVAYFQRGMLYYRMEKYDLAIKDLKEALTQLRGNQLIDYKILGLQFKLFACEVLYNIALMHAKKEEWKKA
EEQLALATNMKSEPRHSKIDKAMESIWKQKLFEPVVIPVGRLFRPNERQVAQLAKKDYLGKATVVASVVH
QDNFSGFAPLQPQSAEPPPRPKTPEIFRALEGEAHRVLFGFVPETPEELQVMPGNIVFVLKKGSDNWATV
MFNGQKGLVPCNYLEPVELRIHPQSQPQEDTSPESDIPPPPNSSPPGRLQLSPGHKQKEPKELKLSVPMP
YMLKVHYKYTVVMETRLGLPYSQLRNMVSKKLALSPEHTKLSYRRRDSHELLLLSEESMKDAWGQVKNYC
LTLWCEHTVGDQGLIDEPIQRENSDASKQTTEPQPKEGTQVVAIFSYEAAQPEDLEFVEGDVILVLSHVN
EEWLEGECKGKVGIFPKAFVEGCAAKNLEGIPREV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010877
ORF Size 1575 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010877.1, NM_010877.2, NM_010877.3, NM_010877.4, NM_010877.5, NP_035007.1
RefSeq Size 2596 bp
RefSeq ORF 1578 bp
Locus ID 17970
UniProt ID O70145
Cytogenetics 1 65.05 cM
MW 59.5 kDa
Summary NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).UniProtKB/Swiss-Prot Function
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"NM_010877" in other vectors (4)
SKU Description Size Price
MC205584 Ncf2 (untagged) - Mouse neutrophil cytosolic factor 2 (Ncf2), (10ug) 10 ug
$490.00
MG208432 Ncf2 (tGFP-tagged) - Mouse neutrophil cytosolic factor 2 (Ncf2) 10 ug
$690.00
MR208432L3 Lenti ORF clone of Ncf2 (Myc-DDK-tagged) - Mouse neutrophil cytosolic factor 2 (Ncf2) 10 ug
$790.00
MR208432L4 Lenti ORF clone of Ncf2 (mGFP-tagged) - Mouse neutrophil cytosolic factor 2 (Ncf2) 10 ug
$790.00

Plasmid Map for MR208432

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208432
Circular map for MR208432

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