Ppp3cb (NM_008914) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR208424
Ppp3cb (Myc-DDK-tagged) - Mouse protein phosphatase 3, catalytic subunit, beta isoform (Ppp3cb)
$289.00
$489.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ppp3cb |
| Synonyms | 1110063J16Rik; Calnb; Cnab; CnAbeta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008914 |
| ORF Size | 1575 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008914.3 |
| RefSeq Size | 3881 bp |
| RefSeq ORF | 1578 bp |
| Locus ID | 19056 |
| UniProt ID | P48453 |
| Cytogenetics | 14 A3 |
| MW | 59.6 kDa |
| Summary | Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Dephosphorylates and activates transcription factor NFATC1. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008914" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC203403 | Ppp3cb (untagged) - Mouse protein phosphatase 3, catalytic subunit, beta isoform (Ppp3cb), (10ug) | 10 ug |
$490.00
|
|
| MG208424 | Ppp3cb (tGFP-tagged) - Mouse protein phosphatase 3, catalytic subunit, beta isoform (Ppp3cb) | 10 ug |
$689.00
|
|
| MR208424L3 | Lenti ORF clone of Ppp3cb (Myc-DDK-tagged) - Mouse protein phosphatase 3, catalytic subunit, beta isoform (Ppp3cb) | 10 ug |
$789.00
|
|
| MR208424L4 | Lenti ORF clone of Ppp3cb (mGFP-tagged) - Mouse protein phosphatase 3, catalytic subunit, beta isoform (Ppp3cb) | 10 ug |
$789.00
|
|
Citations
Calcineurin governs baseline and homeostatic regulations of non-rapid eye movement sleep in mice
Yin, Zhang, Zhou et al
Proc Natl Acad Sci U S A (2025) 122 (4), e2418317122 DOI: 10.1073/pnas.2418317122
Yin, Zhang, Zhou et al
Proc Natl Acad Sci U S A (2025) 122 (4), e2418317122 DOI: 10.1073/pnas.2418317122
Cited by 14
AI Summary: OriGene products contributed to this study by providing mouse open reading frame (ORF) plasmids for CnAα (MR227082), CnAβ (MR208424), CnAγ (MR224578), and PKAα (MR205322), which were used to investigate the roles of these proteins in regulating non-rapid eye movement sleep in mice.
Snippet: Cas9;Sik3Slp-flox/flox with CMV-Cre/+ mice (006054, JAX). Plasmids. Mouse open reading frames (ORFs) of CnAα (MR227082), CnAβ (MR208424), CnAγ (MR224578), and PKAα (MR205322) were purchased from OriGene Technologies. pAAV-Tre-3HA-CnAα/β/γCA were constructed from pAAV-Tre-EGFP by replacing EGFP with ORF sequences of CnAαCA (AA 1-398), CnAβCA (AA 1-408), and CnAγCA (AA 1-391) mutants lack
Calcineurin is a central regulator of Process S (sleep homeostasis) in mice
Liu, Yin, Zhang et al
Research Square (2023)
Liu, Yin, Zhang et al
Research Square (2023)
Snippet: Mouse open reading frames (ORFs) of CnAα (MR227082), CnAβ (MR208424), CnAγ (MR224578), PKAα (MR205322) were purchased from OriGene Technologies. pAAV-hSyn-3HACnAα/β/γ* or pAAV-Tre-3HA-CnAα/β/γ* mutants were constructed from pAAV-hSyn-EGFP (Addgene, 105539) or pAAV-Tre-EGFP by replacing GFP with DNA sequences of CnAα (AA 1
Showing 1-2 of 2 papers.
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