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Ppp2r3a (BC059852) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208419
Ppp2r3a (Myc-DDK-tagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha (cDNA clone MGC:69692 IMAGE:6402777)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $489.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppp2r3a
Synonyms PP2A B''-PR59; PP2A PR59; Ppp2r3; Ppp2r3a; PR59
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208419 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCGCTGACGCCGCGGCTACAGCTGAAGGTGGACGAGCTCTTCCGGCGCTGGCTCGGCGATCCTC
ACACGCAGCGCGCGCTCAGCCACGCCCTCCGCCGCATCCGGGACCCAGGGACGACCTCTGACCCCGCCGC
CGCCGCGACCCCCGATGCGGACCCCGGAAACACGACCTCCGACCCTAACCCCGCCTCCCGACCGCTGCCG
CGGCCCGCGCTGAGAACGACGGGACCCAGGACGGTCCATGCCCACAGGAAGGAGGAGCCCCCGCCCCAGT
CCCCACCCCCTGGCCACAGCGTCCCCGCCTTCTACTTCCCTTGCGGTCGACCCCCGCCCCGTCCCCAGGA
CACGGAGGATGCCATCGCCCTCGTGGAGTGCGCGTTCGAGGGGCTGCCTCGTGGGAGGGCGGGTCTGGGC
GACATGGCCGTGGTTGCCAAGGCCTGTGGCTGCCCTCTGTACTGGAAGGCCCCGCTGTTCTACGCTGCGG
GCGGCGAGCGCACAGGCTCTGTATCCGTGCACATGTTCGTAGCTATGTGGCGCAAAGTCCTGCTGACCTG
CCATGATGATGCTGCGCGGTTCGTGCGGCTGCTGGGCCACCCCGGGTGTAGTGGCCTCATCCAGGAGGAC
TTTGTGCCCTTCCTGCAGGATGTGGTGAACTCGCACCCGGGCCTGGCCTTCCTGAGGGCAGCGAAGGACT
TCCACTCACGTTACATCACCACAGTGATTCAGAGGATCTTCTACACAGTAAACCGGTCATGGTCAGGGAT
GATCTCGCGTGAGGAGCTAAGGCGCAGCAGCTTCCTGCAGGCTGTGTCACAGTTGGAGGTAGAGCCTGAC
ATCAACCGCATGACGTCGTTCTTCTCTTATGAGCATTTCTATGTCATCTACTGCAAGTTCTGGGAGCTCG
ACCTCGACCGCGACCTCACCATTGACAGGAGCGACCTCGCACGCCATGGGGACGGGGCCATCTCCTCCAG
AATGATTGACCGCATCTTCTCAGGGGCCGTCACCAGGGCGAGGCTGCCCCGAAAGGTCGGGAAGCTGAGC
TACGCAGACTTTGTATGGTTCCTGCTCTCCGAGGAGGACAAGACTACGCCCACCAGCACCGAGTACTGGT
TCCGTTGCATGGACCTGGACGGTGACGGCGCTCTCTCCATGTTTGAGCTCGAGTTCTTCTATGAGGAGCA
GGCCCAGCGCATGGCCGCCCGGGGTGTGGAGCCCCTCCCCTTCCACGACCTTGCACGCCAGGTGCTCGAC
CTCGTCGCCCCGCGCTGCCCCGGCCGGATCACGCTGCGCGACCTGAAGCAGTGCGGCCTGGCGGGTGAGT
TCTTCGACGCCTTCTTCAACGTGGACAAGTACCTGGCACGGGAGCAGCGCGAGCAGGCGGGGACTCCGCA
GGACACTGACTCCGACCCCGCGGCATCTGCCTGGGACAGGTATGCCGCCGAGGAGTACGACTTCCTCGTG
GCCGAGGAGGCCATGGCAGAGGACGACGACGACCATGACGAAGGGTCCGACCCCATAGATCTCTACGGCC
TTGCTGACGAGGACTGCGACGACCTGGAGCCTCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208419 protein sequence
Red=Cloning site Green=Tags(s)

MAPLTPRLQLKVDELFRRWLGDPHTQRALSHALRRIRDPGTTSDPAAAATPDADPGNTTSDPNPASRPLP
RPALRTTGPRTVHAHRKEEPPPQSPPPGHSVPAFYFPCGRPPPRPQDTEDAIALVECAFEGLPRGRAGLG
DMAVVAKACGCPLYWKAPLFYAAGGERTGSVSVHMFVAMWRKVLLTCHDDAARFVRLLGHPGCSGLIQED
FVPFLQDVVNSHPGLAFLRAAKDFHSRYITTVIQRIFYTVNRSWSGMISREELRRSSFLQAVSQLEVEPD
INRMTSFFSYEHFYVIYCKFWELDLDRDLTIDRSDLARHGDGAISSRMIDRIFSGAVTRARLPRKVGKLS
YADFVWFLLSEEDKTTPTSTEYWFRCMDLDGDGALSMFELEFFYEEQAQRMAARGVEPLPFHDLARQVLD
LVAPRCPGRITLRDLKQCGLAGEFFDAFFNVDKYLAREQREQAGTPQDTDSDPAASAWDRYAAEEYDFLV
AEEAMAEDDDDHDEGSDPIDLYGLADEDCDDLEPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC059852
ORF Size 1575 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC059852, AAH59852
RefSeq Size 2172 bp
RefSeq ORF 1577 bp
Locus ID 19054
Cytogenetics 9 F4
MW 59.1 kDa
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"BC059852" in other vectors (4)
SKU Description Size Price
MC217734 Ppp2r3a (untagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha (cDNA clone MGC:69692 IMAGE:6402777),, (10ug) 10 ug
$539.00
MG208419 Ppp2r3a (tGFP-tagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha (cDNA clone MGC:69692 IMAGE:6402777) 10 ug
$689.00
MR208419L3 Lenti ORF clone of Ppp2r3a (Myc-DDK-tagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha (cDNA clone MGC:69692 IMAGE:6402777) 10 ug
$789.00
MR208419L4 Lenti ORF clone of Ppp2r3a (mGFP-tagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha (cDNA clone MGC:69692 IMAGE:6402777) 10 ug
$789.00

Plasmid Map for MR208419

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208419
Circular map for MR208419

file-alt Citations

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