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Pak2 (NM_177326) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208417
Pak2 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 2 (Pak2)
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $488.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pak2
Synonyms 5330420P17Rik; A130002K10Rik; AI836325; D16Ertd269e; mKIAA4182; PAK-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208417 representing NM_177326
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGATAACGGAGAGCTAGAAGACAAGCCCCCAGCACCTCCAGTTCGGATGAGCAGTACCATTTTTA
GCACCGGAGGAAAAGATCCTTTATCAGCCAATCACAGTTTGAAACCTTTGCCTTCTGTTCCAGAGGAAAA
AAAACCCAGGAACAAAATCATCTCCATATTCTCTGGCACAGAAAAAGGAAGTAAAAAGAAAGAAAAAGAA
CGGCCAGAGATTTCTCCCCCATCTGATTTTGAGCACACCATCCATGTTGGCTTTGATGCTGTTACGGGAG
AGTTCACTGGCATGCCAGAACAGTGGGCGCGGCTGTTGCAGACCTCCAACATTACCAAACTCGAGCAGAA
GAAGAACCCTCAGGCAGTGCTGGATGTCTTGAAGTTCTACGACTCCAACACTGTGAAACAGAAGTACCTG
AGTTTCACTCCTCCTGAGAAAGATGGCTTCCCTTCTGGAACACCAGCACTGAACACCAAGGGGTCAGAGA
CATCAGCTGTAGTGACAGAGGAAGATGATGATGATGAAGACGCTGCTCCTCCCGTCATTGCCCCTCGGCC
AGATCATACAAAATCAATTTACACACGGTCTGTCATCGACCCCATTCCTGCTCCAGTTGGTGATTCTAAT
GTTGACAGTGGTGCCAAGTCTTCAGACAAACAGAAAAAGAAAGCCAAGATGACCGATGAAGAGATTATGG
AGAAATTAAGAACTATTGTGAGCATAGGGGACCCAAAGAAAAAATACACAAGATATGAAAAAATTGGGCA
AGGGGCTTCTGGAACAGTTTTTACTGCCACTGATGTGGCCCTGGGGCAAGAGGTTGCTATCAAGCAGATT
AATTTACAGAAACAACCAAAGAAGGAATTGATCATTAATGAAATTCTGGTGATGAAAGAGTTAAAGAATC
CCAACATAGTTAACTTCTTGGACAGTTACCTGGTAGGAGATGAGTTGTTTGTGGTAATGGAGTACCTCGC
TGGTGGGTCCCTCACTGATGTTGTAACAGAAACCTGCATGGACGAAGCGCAGATTGCCGCCGTGTGCAGA
GAGTGTTTACAGGCGTTGGAGTTTTTACATGCTAATCAAGTGATCCACAGAGACATCAAAAGTGACAATG
TGCTTTTGGGAATGGAAGGCTCAGTTAAACTTACTGACTTCGGCTTCTGTGCCCAGATCACTCCTGAACA
GAGCAAACGCAGTACTATGGTTGGAACACCGTACTGGATGGCACCAGAGGTGGTGACACGGAAAGCCTAT
GGTCCCAAAGTTGACATATGGTCTCTGGGCATCATGGCTATCGAGATGGTTGAAGGAGAGCCTCCATACC
TCAACGAAAATCCTCTGCGGGCATTATACCTGATAGCTACAAATGGAACTCCTGAACTTCAGAATCCAGA
AAAACTTTCCCCAATATTTCGGGATTTCTTAAATCGGTGTTTGGAAATGGATGTGGAGAAAAGGGGTTCG
GCCAAGGAACTGTTACAGCATCCTTTCCTGAAACTGGCCAAACCATTGTCTAGCTTGACGCCACTGATCC
TGGCAGCTAAAGAAGCAATGAAGAGTAACCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208417 representing NM_177326
Red=Cloning site Green=Tags(s)

MSDNGELEDKPPAPPVRMSSTIFSTGGKDPLSANHSLKPLPSVPEEKKPRNKIISIFSGTEKGSKKKEKE
RPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSNTVKQKYL
SFTPPEKDGFPSGTPALNTKGSETSAVVTEEDDDDEDAAPPVIAPRPDHTKSIYTRSVIDPIPAPVGDSN
VDSGAKSSDKQKKKAKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQI
NLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCR
ECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAY
GPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGS
AKELLQHPFLKLAKPLSSLTPLILAAKEAMKSNR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177326
ORF Size 1572 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177326.3, NP_796300.1
RefSeq Size 4135 bp
RefSeq ORF 1575 bp
Locus ID 224105
UniProt ID Q8CIN4
Cytogenetics 16 22.4 cM
MW 58.4 kDa
Summary Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation (By similarity).UniProtKB/Swiss-Prot Function
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"NM_177326" in other vectors (3)
SKU Description Size Price
MC217513 Pak2 (untagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 2 (Pak2), (10ug) 10 ug
$538.00
MR208417L3 Lenti ORF clone of Pak2 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 2 (Pak2) 10 ug
$788.00
MR208417L4 Lenti ORF clone of Pak2 (mGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 2 (Pak2) 10 ug
$788.00

Plasmid Map for MR208417

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file-download TXT (FASTA format)

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Cloning scheme for MR208417
Circular map for MR208417

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