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Alpl (NM_007431) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208392
Alpl (Myc-DDK-tagged) - Mouse alkaline phosphatase, liver/bone/kidney (Alpl)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $489.00
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Other products for "Alpl or NM_007431"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Alpl
Synonyms Ak; Akp; Akp-2; Akp2; ALP; APTNAP; T; TNAP; TNSALP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208392 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCTCACCATTTTTAGTACTGGCCATCGGCACCTGCCTTACCAACTCTTTTGTGCCAGAGAAAGAGA
GAGACCCCAGTTACTGGCGACAGCAAGCCCAAGAGACCTTGAAAAATGCCCTGAAACTCCAAAAGCTCAA
CACCAATGTAGCCAAGAATGTCATCATGTTCCTGGGAGATGGTATGGGCGTCTCCACAGTAACCGCTGCC
CGAATCCTTAAGGGCCAGCTACACCACAACACGGGCGAGGAGACCCGGCTGGAGATGGACAAATTCCCCT
TTGTGGCCCTCTCCAAGACATATAACACCAACGCTCAGGTCCCTGACAGCGCGGGCACTGCCACTGCCTA
CTTGTGTGGCGTGAAGGCCAACGAGGGCACAGTGGGAGTGAGCGCAGCCACAGAGCGCACGCGATGCAAC
ACCACTCAGGGCAATGAGGTCACATCCATCCTGCGCTGGGCCAAGGATGCTGGGAAGTCCGTGGGCATTG
TGACTACCACTCGGGTGAACCACGCCACACCCAGTGCAGCCTACGCACACTCGGCCGATCGGGACTGGTA
CTCGGATAACGAGATGCCACCAGAGGCTCTGAGCCAGGGCTGCAAGGACATCGCATATCAGCTAATGCAC
AATATCAAGGATATCGACGTGATCATGGGTGGCGGCCGGAAATACATGTACCCGAAGAACAGAACTGATG
TGGAATACGAACTGGATGAGAAGGCCAGGGGTACAAGGCTAGATGGCCTGGATCTCATCAGTATTTGGAA
GAGCTTTAAACCCAGACACAAGCATTCCCACTATGTCTGGAACCGCACTGAACTGCTGGCCCTTGACCCC
TCCAGGGTGGACTACCTCTTAGGTCTCTTTGAGCCCGGGGACATGCAGTATGAATTGAATCGGAACAACC
TGACTGACCCTTCGCTCTCCGAGATGGTGGAGGTGGCCCTCCGGATCCTGACCAAAAACCTCAAAGGCTT
CTTCTTGCTGGTGGAAGGAGGCAGGATTGACCACGGACATCATGAGGGTAAGGCCAAGCAGGCTCTGCAT
GAAGCAGTGGAGATGGACCAGGCCATTGGCAAGGCAGGCGCCATGACATCCCAGAAAGACACCTTGACTG
TGGTTACTGCTGATCATTCCCACGTTTTCACATTCGGTGGATACACCCCCCGGGGCAACTCCATCTTTGG
TCTGGCTCCCATGGTGAGCGACACGGACAAGAAGCCCTTCACGGCCATCCTATATGGTAACGGGCCTGGC
TACAAGGTGGTGGACGGTGAACGGGAAAATGTCTCCATGGTAGATTACGCTCACAACAACTACCAGGCCC
AGTCCGCTGTTCCCCTGCGCCATGAGACCCACGGTGGAGAAGACGTGGCGGTCTTTGCCAAGGGCCCGAT
GGCACACCTGCTTCACGGCGTCCATGAGCAGAACTACATTCCCCATGTGATGGCGTATGCCTCCTGCATT
GGGGCCAACCTTGACCACTGTGCCTGGGCCGGCTCTGGGAGCGCACCCTCCCCAGGGGCCCTGCTGCTTC
CACTGGCTGTGCTCTCCCTACGCACCCTGTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208392 protein sequence
Red=Cloning site Green=Tags(s)

MISPFLVLAIGTCLTNSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAA
RILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCN
TTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMH
NIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDP
SRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALH
EAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPG
YKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCI
GANLDHCAWAGSGSAPSPGALLLPLAVLSLRTLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007431
ORF Size 1572 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007431.3
RefSeq Size 2524 bp
RefSeq ORF 1575 bp
Locus ID 11647
UniProt ID P09242
Cytogenetics 4 70.02 cM
MW 57.5 kDa
Summary This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack this enzyme show symptoms of osteomalacia, softening of the bones. In humans, mutations in this gene are associated with hypophosphatasia, an inherited metabolic bone disease in which deficiency of this enzyme inhibits bone mineralization leading to skeletal defects. Mutations in the mouse gene mirror the symptoms of human hypophosphatasia. A pseudogene of this gene is present on chromosome X. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2015
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"NM_007431" in other vectors (3)
SKU Description Size Price
MG208392 Alpl (tGFP-tagged) - Mouse alkaline phosphatase 2, liver (Akp2) 10 ug
$689.00
MR208392L3 Lenti ORF clone of Alpl (Myc-DDK-tagged) - Mouse alkaline phosphatase, liver/bone/kidney (Alpl) 10 ug
$789.00
MR208392L4 Lenti ORF clone of Alpl (mGFP-tagged) - Mouse alkaline phosphatase, liver/bone/kidney (Alpl) 10 ug
$789.00

Plasmid Map for MR208392

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208392
Circular map for MR208392

file-alt Citations

Mitochondrial TNAP controls thermogenesis by hydrolysis of phosphocreatine
Sun, Rahbani, Jedrychowski et al
Nature (2021) 593 (7860), 580-585 DOI: 10.1038/s41586-021-03533-z
Cited by 141
AI Summary: OriGene’s mouse Alpl cDNA clone (MR208392) was used to amplify the open-reading frame for AdALPL, enabling the study of TNAP’s role in mitochondrial phosphocreatine hydrolysis and thermogenesis.
Snippet: For AdALPL, the open-reading frame (ORF) of mouse Alpl was amplified from a cDNA clone (Origene, MR208392),
Showing 1-1 of 1 papers.
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