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AI450540 (BC062949) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208376
AI450540 (Myc-DDK-tagged) - Mouse expressed sequence AI450540 (cDNA clone MGC:77957 IMAGE:6839812)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $487.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol AI450540
Synonyms AI450540; mKIAA1600
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208376 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCTCCAAGCTTACGTCCATCCTGCAGCATGCCGTGGAGGCGCTCGCACCGTCTCTCCCGTTACAAG
AAGATTTTGTTTATCACTGGAAGGCGATTACACACTACTACATAGAAACTTCAGATGATAAAGCCCCAGT
GACCGACACAAACATCCCATCCCACCTGGAACAGATGTTAGACATCCTGGTCCAAGAAGAAAATGAGCGG
GAATCTGGAGAGACAGGGCCATGCATGGAATACCTGCTCCATCACAAGATCTTGGAAACGTTATACACCT
TAGGGAAAGCCGACTGCCCTCCAGGAATGAAGCAGCAGGTTTTGGTGTTCTATACCAAGCTTCTGGGACG
AATCCGGCAGCCCCTGCTTCCACACATCAACGTACACAGGCCTGTGCAGAAATTAATTCGACTCTGTGGT
GAGGTCCTTGCAACGCCAACGGAGAATGAAGAGATCCAGTTTCTCTGCATCGTGTGTGCAAAGCTGAAAC
AGGATCCCTACTTGGTCAACTTTTTCCTGGAGAACAAATCAAAGTCCCTGGTGTCCAGAGGAGCTCTGAG
TGTCATCTCAGAGGATGGCCCAAAAGGTCAGGACCCGGGGTCAGGAGACGTCAGCCAGTGCCAGCAGCCC
CAGGAGCTGTCAGGTGCTACTGGGGTGGAGCCCACGGAGTCGGAGGAAGAGCCCCCTCACCAGATGGATG
ACCTGTCTGCAAGCCTAGATGACCTCAATGTCACCTCACTGCCGGAGGCCTCTGCCGTCCGGCCAAACCA
GGACTACAACTTAGTGAATTCTTTGTTAAACCTGACAAGAAGTCCTGACGGCCGGATAGCTGTGAAGGCC
TGCGAGGGCTTGATGCTCTTGGTGAGCCTACCGGAGCCTGCGGCTGCCAAGTGCCTTGCTCAGAGCACCT
GCCTGTGCGAGCTGCTGACCGGCAGACTCACCTCCCTGTACAAGGCTCTACCTCAGTCAGTGGACCCCTT
AGACATTGAAACCGTGGAGGCAGTTAACTGGGGGTTGGACTCATACAGCCATAAAGAAGACGCTTCGGCG
TTCCCGGGGAAGCGGGCCTTAATTTCATTTCTTTCCTGGTTCGATTACTGTGATCAGCTCATCAAGGAGG
CACAAAAGACTGCTGCTGTTGCTCTCGCCAAAGCCATCCACGAAAGGTTCTTTGTTGGTGTCATGGAACC
CCAGCTGATGCAGACCTCTGAGATGGGGATTCTGACCTCGACGGCCTTGCTGCACCGCATCGTCCGGCAA
GTGACCTCAGACGTCCTGCTTCAGGAAATGGTGGTCTTCATCCTCGGAGAACAGAGGGAGCCAGAAACTC
TGTCAGAAATTAGCAGACATCCTCTAAGACATAGGTTAATCGAACATTGCGATCACATATCTGATGAGAT
CAGCATCATGACCCTGAGGATGTTTGAGCATCTCCTACAAAAGCCCAATGAACACATCCTCTACAACCTG
GTCTTGAGAAACCTGGAAGAAAGAAACTATACGGAGAACAAACCTTCCTGCCCGGAAGACAAGGACGTGG
TGGAGAACGGGCTGATAGCAGGCGCCGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208376 protein sequence
Red=Cloning site Green=Tags(s)

MFSKLTSILQHAVEALAPSLPLQEDFVYHWKAITHYYIETSDDKAPVTDTNIPSHLEQMLDILVQEENER
ESGETGPCMEYLLHHKILETLYTLGKADCPPGMKQQVLVFYTKLLGRIRQPLLPHINVHRPVQKLIRLCG
EVLATPTENEEIQFLCIVCAKLKQDPYLVNFFLENKSKSLVSRGALSVISEDGPKGQDPGSGDVSQCQQP
QELSGATGVEPTESEEEPPHQMDDLSASLDDLNVTSLPEASAVRPNQDYNLVNSLLNLTRSPDGRIAVKA
CEGLMLLVSLPEPAAAKCLAQSTCLCELLTGRLTSLYKALPQSVDPLDIETVEAVNWGLDSYSHKEDASA
FPGKRALISFLSWFDYCDQLIKEAQKTAAVALAKAIHERFFVGVMEPQLMQTSEMGILTSTALLHRIVRQ
VTSDVLLQEMVVFILGEQREPETLSEISRHPLRHRLIEHCDHISDEISIMTLRMFEHLLQKPNEHILYNL
VLRNLEERNYTENKPSCPEDKDVVENGLIAGAV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC062949
ORF Size 1569 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC062949, AAH62949
RefSeq Size 3797 bp
RefSeq ORF 1571 bp
Locus ID 226252
Cytogenetics 19 D2
MW 58.5 kDa
Summary May be required for proper functioning of the nervous system.UniProtKB/Swiss-Prot Function
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"BC062949" in other vectors (4)
SKU Description Size Price
MC218002 AI450540 (untagged) - Mouse expressed sequence AI450540 (cDNA clone MGC:77957 IMAGE:6839812), (10ug) 10 ug
$537.00
MG208376 AI450540 (tGFP-tagged) - Mouse expressed sequence AI450540 (cDNA clone MGC:77957 IMAGE:6839812) 10 ug
$687.00
MR208376L3 Lenti ORF clone of AI450540 (Myc-DDK-tagged) - Mouse expressed sequence AI450540 (cDNA clone MGC:77957 IMAGE:6839812) 10 ug
$787.00
MR208376L4 Lenti ORF clone of AI450540 (mGFP-tagged) - Mouse expressed sequence AI450540 (cDNA clone MGC:77957 IMAGE:6839812) 10 ug
$787.00

Plasmid Map for MR208376

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208376
Circular map for MR208376

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