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Dync1li1 (NM_146229) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208374
Dync1li1 (Myc-DDK-tagged) - Mouse dynein cytoplasmic 1 light intermediate chain 1 (Dync1li1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $488.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dync1li1
Synonyms 1110053F02Rik; Dnclic1; LIC-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208374 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGTGGGGCGAGTCGGCTCGTTCGGTTCATCTCCACCCGGGCTAGCCTCGACCTACGCCAGCG
GACCCCTAGCCAACGAGCTAGCGTCGGGCAGCGGCGGCCCCGCGGCGGGCGACGATGAGGACGGGCAGAA
CCTTTGGTCCTGCATCCTCAGCGAGGTCTCCACGCGCTCGCGCTCCAAGCTCCCGACCGGGAAGAACGTG
CTGCTGCTGGGTGAAGATGGAGCTGGGAAGACAAGCCTCATAAGAAGAATTCAGGGCATAGAGGAGTACA
AGAAGGGAAGAGGACTGGAGTACCTGTACTTAAATGTGCACGACGAAGACAGGGATGATCAAACAAGATG
TAATGTATGGATCTTAGATGGAGATCTGTACCACAAAGGGCTACTCAAGTTCTCTCTGGATGCCCTTTCT
CTGAGGGACACTCTGGTCATGTTGGTTGTTGATATGTCAAAGCCTTGGACTGCTTTGGATTCCTTACAGA
AATGGGCAAGTGTGGTGAGAGAACATGTCGACAAGCTGAAAATCCCCCCTGAAGAGATGAAGGAAATGGA
GCAGAAGTTGATTAGAGACTTCCAAGAGTACGTGGAGCCAGGAGAAGACTTCCCAGCCTCCCCTCAGCGA
AGAACCACTGGTGCGCAGGAGGACAGAGGTGACAGTGTTGTCCTGCCCCTGGGTGCAGACACACTCACAC
ACAACCTGGGCCTACCAGTGCTCGTAGTCTGTACGAAGTGTGATGCCATTAGTGTATTGGAGAAAGAGCA
TGACTACAGAGATGAACACTTCGATTTTATCCAGTCTCACATCCGCAAGTTCTGTTTACAGTATGGCGCA
GCGCTGATTTACACTTCAGTAAAAGAGAACAAGAACATAGACTTAGTTTATAAATACATCGTCCAGAAGC
TGTACGGGTTCCCGTACAAGATCCCTGCGGTGGTGGTGGAGAAGGACGCAGTGTTTATTCCAGCAGGGTG
GGATAATGATAAGAAAATAGGAATATTACATGAAAATTTTCAGACTTTGAAAGTAGAAGATAATTTTGAA
GACATCATAACCAAACCTCCTGTCAGAAAGTTCGTGCATGAGAAGGAGATCATGGCAGAAGATGACCAAG
TGTTTCTGATGAAGCTACAGTCTCTTTTAGCAAAGCAACCTCCAACTGCAGCTGGAAGGCCTGTGGATGC
ATCACCAAGAGTCCCTGGAGGCTCCCCTCGAACACCAAACAGATCTGTGTCATCCAATGTTGCCAGTGTG
TCCCCCATCCCTGCTGGATCCAAAAAAATTGATCCAAACATGAAAGCTGGAGCGACCAGCGAAGGGGTCC
TGGCGAATTTCTTCAACAGTCTGTTGAGTAAGAAGACTGGCTCTCCTGGAGGCCCCGGGGTCGGCGGCAG
TCCTGGAGGAGGGGCTGCAGGTGCAAGCCCCAGTTTGCCACCGTCCGCCAAAAAGTCAGGCCAGAAACCT
GTGCTTTCAGATGTTCACGCAGAGCTAGACAGAATCACACGGAAACCTGCTTCTGTTTCTCCTACAACAC
CTACATCTCCTACGGAAGGAGAAGCCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208374 protein sequence
Red=Cloning site Green=Tags(s)

MAAVGRVGSFGSSPPGLASTYASGPLANELASGSGGPAAGDDEDGQNLWSCILSEVSTRSRSKLPTGKNV
LLLGEDGAGKTSLIRRIQGIEEYKKGRGLEYLYLNVHDEDRDDQTRCNVWILDGDLYHKGLLKFSLDALS
LRDTLVMLVVDMSKPWTALDSLQKWASVVREHVDKLKIPPEEMKEMEQKLIRDFQEYVEPGEDFPASPQR
RTTGAQEDRGDSVVLPLGADTLTHNLGLPVLVVCTKCDAISVLEKEHDYRDEHFDFIQSHIRKFCLQYGA
ALIYTSVKENKNIDLVYKYIVQKLYGFPYKIPAVVVEKDAVFIPAGWDNDKKIGILHENFQTLKVEDNFE
DIITKPPVRKFVHEKEIMAEDDQVFLMKLQSLLAKQPPTAAGRPVDASPRVPGGSPRTPNRSVSSNVASV
SPIPAGSKKIDPNMKAGATSEGVLANFFNSLLSKKTGSPGGPGVGGSPGGGAAGASPSLPPSAKKSGQKP
VLSDVHAELDRITRKPASVSPTTPTSPTEGEAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146229
ORF Size 1569 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146229.2, NP_666341.1
RefSeq Size 2046 bp
RefSeq ORF 1572 bp
Locus ID 235661
UniProt ID Q8R1Q8
Cytogenetics 9 F3
MW 56.6 kDa
Summary Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress through the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores (By similarity).UniProtKB/Swiss-Prot Function
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"NM_146229" in other vectors (4)
SKU Description Size Price
MC201651 Dync1li1 (untagged) - Mouse dynein cytoplasmic 1 light intermediate chain 1 (Dync1li1), (10ug) 10 ug
$488.00
MG208374 Dync1li1 (tGFP-tagged) - Mouse dynein cytoplasmic 1 light intermediate chain 1 (Dync1li1) 10 ug
$688.00
MR208374L3 Lenti ORF clone of Dync1li1 (Myc-DDK-tagged) - Mouse dynein cytoplasmic 1 light intermediate chain 1 (Dync1li1) 10 ug
$788.00
MR208374L4 Lenti ORF clone of Dync1li1 (mGFP-tagged) - Mouse dynein cytoplasmic 1 light intermediate chain 1 (Dync1li1) 10 ug
$788.00

Plasmid Map for MR208374

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR208374
Circular map for MR208374

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