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Sgo1 (NM_028232) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208287
Sgol1 (Myc-DDK-tagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $481.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sgo1
Synonyms 3300001M08Rik; C81037; Sgo1; Sgol1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208287 representing NM_028232
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAAGGAAAGGTGTCAGAAAAGGTCCTTTCAAGATACCCTTGAAGACATTAAGAATCGAATGAAAG
AAAAAAGGAATAAAAATTTGGCGGGGATTGGGAAACGCAAGTCCTTTATTGTTGCACCGGGCCAAGTACC
CACTAACACTGCTACACTACTGAGATATTACCAAGATAACAACAGGTTGTTAGTCTTGGCTTTGGAAAAT
GAGAAATCCAAAGTGAGAGAAGCACAGGATGTCATCCTGCAACTGAGAAAAGAATGCTACTACCTTACTT
GTCAGCTGTATGCATTGAAAGAGAAGCTAACTTCCCGACAAAGTGAAGAAACTACTCAGAACTGGAAAGG
ACGTCCCTCAGACGTGGTCTCCAGCATTGACAATACGACCAGGGACTTGTCAGGGAAGTCCTTACAGCAA
ATTGCTGTTGAAGAAACTGATTGTCCTTACCAAACCACAGAACCAAGTCCTGCTGTTACTCCAGAGACAC
AGGGTTGCGATTTTGATTCAGGTAAAGTTGAGTCTACTGATGAAGTCTTACCCAGAACTATATCTATCCG
TCGCCATTTAAGGAAAGATTTTAGTAATATAAGCCACTCCACGACTTTGGAGGATTGTAAAGCCAGTCCA
AGAGTGGCACAGTCTCTGGAAGTTAAAGGAAGTAGATGTAGAGAAGTAACCGTAACCCTGCACAGACTTG
AAAATGTTTGTCTGTGGAACAAAGACCAAATTAGCTTATGTTCTAGACTGATTAACCCAGCAAAGATTAC
TGAAACAGAAGTCATTTTATCATCTAAACCTGAACAAATAGAAAGCAAGCATAAACGTGCACGAAAAAGA
AGAGCAGAGCAAAGAAGAACCAAGCAGAGATGCAAATCAAAATCCTCATTGAGGAGTAAGGGGAACAAAA
ACAAAGATAAGCAGGGTTTACCCCCTACTACACTGGATGGAGGTATTGGTTCCTGTGATGCTTACGATTT
TAATCTAAAAGGGACGGTCCACCCCACCCCTTTCCGACAAAAAATGAACAATGGCTGCAACAAAGAAACG
GATAGCAGCAACTCAGAAGTGAGTGACCTCGAATGCAGTACCTCTGAGGATGAGTCTGATGACCTCTACC
TGCCTCCCTCCAAGCGCTTGCGAGACTACAGAGAGTCAGAGAGAGCAGTTACCAGGCCTCGGTCTAAAAG
AGGACTTCAGTACCCAGATGGGAAAGAGAGGAAGGAGGTGCTGCCATCTACAGCTCCTACTGGTATCCCA
CCTGAGACTCAAGAGTCACCTCGTTGTAGCCTAAAGGATGTCACCAATATCCTGCAGTGTCCTAGAGTGA
AGATCAGGAAGCCTTCTCTGCCTCCAAAGCGGCGTGAAGACAGCCCAGCAGTGGCTCTGACTAAACGCAG
GTGTAGCACCATCAAAAGCTATAAAGAGCCAACACTCGCTTCAAAGCTAAGAAGAGGGGACCCTTTCACG
GACTTGTGTTTCTTGAATTCTCCTATTTTCAAGCAGAAAAGGGGTATGAGATGTCCTAAAAGAAGAACCA
AGCAAACACAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208287 representing NM_028232
Red=Cloning site Green=Tags(s)

MAKERCQKRSFQDTLEDIKNRMKEKRNKNLAGIGKRKSFIVAPGQVPTNTATLLRYYQDNNRLLVLALEN
EKSKVREAQDVILQLRKECYYLTCQLYALKEKLTSRQSEETTQNWKGRPSDVVSSIDNTTRDLSGKSLQQ
IAVEETDCPYQTTEPSPAVTPETQGCDFDSGKVESTDEVLPRTISIRRHLRKDFSNISHSTTLEDCKASP
RVAQSLEVKGSRCREVTVTLHRLENVCLWNKDQISLCSRLINPAKITETEVILSSKPEQIESKHKRARKR
RAEQRRTKQRCKSKSSLRSKGNKNKDKQGLPPTTLDGGIGSCDAYDFNLKGTVHPTPFRQKMNNGCNKET
DSSNSEVSDLECSTSEDESDDLYLPPSKRLRDYRESERAVTRPRSKRGLQYPDGKERKEVLPSTAPTGIP
PETQESPRCSLKDVTNILQCPRVKIRKPSLPPKRREDSPAVALTKRRCSTIKSYKEPTLASKLRRGDPFT
DLCFLNSPIFKQKRGMRCPKRRTKQTQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028232
ORF Size 1551 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028232.2, NP_082508.1
RefSeq Size 3609 bp
RefSeq ORF 1554 bp
Locus ID 72415
UniProt ID Q9CXH7
Cytogenetics 17 C
MW 59 kDa
Summary Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Involved in centromeric enrichment of AUKRB in prometaphase.UniProtKB/Swiss-Prot Function
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"NM_028232" in other vectors (4)
SKU Description Size Price
MC217322 Sgol1 (untagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1), (10ug) 10 ug
$530.00
MG208287 Sgol1 (tGFP-tagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1) 10 ug
$681.00
MR208287L3 Lenti ORF clone of Sgol1 (Myc-DDK-tagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1) 10 ug
$781.00
MR208287L4 Lenti ORF clone of Sgol1 (mGFP-tagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1) 10 ug
$781.00

Plasmid Map for MR208287

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208287
Circular map for MR208287

file-alt Citations

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