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Pdia5 (NM_028295) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208279
Pdia5 (Myc-DDK-tagged) - Mouse protein disulfide isomerase associated 5 (Pdia5)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $482.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pdia5
Synonyms 2700053F16Rik; AU015525; Pdir
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208279 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGGGCGTGGGGGCTGCTACTGGCGATTGGGGTGGTCCTGCCGACATGGCTGTCCTCCACAAAGG
TCTCTTCGCTCATTGAGAGAATCTCCGACCCTAAGGACTTGAAGAAACTTCTCAGGACCCGCAATAACGT
GCTGGTGCTTTACTCGGAATCCGAGGTAGCAGCTGAAAGTCATCTTAAGCTCCTGTCCACCGTGGCCCAG
GCAGTGAAAGGCCAAGGCACCGTCTGCTGGGTGGACTGCGGTGACGCAGAGAGTAGAAAATTGTGCAAGA
AGATGAAAGTTGACCTCAGCCCAAAGGACAAAAAGATTGAATTATTCCATTACCAGGACGGCGCCTTTCA
CATGCAGTATGACCGAGCTGTGACGTTGAAGTCCATAGTGGCCTTTCTGAAAGACCCTAAAGGTCCCCCA
CTGTGGGAAGAAGATCCTGGAGCCAAAGATGTTGTGCACATCGACAGTGAGAAGGACTTCAGACGACTCC
TGAAGAGAGAAGAGAAGCCACTGCTCATGATGTTCTATGCTCCCTGGTGCAGCATGTGCAAGAGAATCAT
GCCACATTTCCAGAAGGCTGCCACCCAAGTGCGAGGCCACATTGTCCTGGCTGGGATGAACGTCTACCCA
TCAGAGTTCGAGAACATCAAGGAAGAGTACAATGTCCGCGGCTACCCTACCATCTGCTATTTCGAGAAGG
GGCGGTTTCTGTTCCCGTATGAGAACTATGGGTCCACGGCCGAGGACATCGTGGAGTGGTTGAAGAATCC
ACTGCCACCACAGCCCCAGGTCCCCGAGACTCCCTGGGCAGATGAGGGCGGCTCCGTTTATCACCTGACC
GATGAAGACTTTGACCAGTTTGTGAAGGAGCACTCCTCTGTCCTCGTCATGTTCCACGCCCCATGGTGTG
GACATTGTAAGAAAATGAAGCCAGAGTTTGAGAGTGCTGCTGAAGTCCTCCACGGAGATGCTGAGAGCTC
TGGTGTACTTGCAGCTGTCGATGCCACCGTCAACGAGGCCCTGGCAGGAAGATTTCACATCTCAGCGTTT
CCTACCCTGAAGTATTTTAAGAATGGCGAGCAGCAAGCGGTGCCTGCGCTCAGAACAAAGAAGAAGTTTA
TTGAGTGGATGCAGAACCCTGAGGCTCCCCCACCTCCAGAGCCCACGTGGGAAGAACAGCAGACAAGCGT
GCTACATCTGGTGGGGGATAACTTCCGGGATACCCTGAAGAAGAAGAAACACACCTTGGTCATGTTCTAT
GCCCCTTGGTGCCCACACTGTAAGAAGGTCATCCCCCACTTCACTGCCACAGCCGATGCCTTCAAAGAGG
ACCGCAAGATTGCCTGTGCTGCTGTAGACTGTGTCAAGGACAAGAACCAAGACCTGTGTCAGCAGGAGGC
CGTGAAGGCCTACCCCACCTTCCACTACTACCACTACGGGAAGCTCGTAGAAAAGTATGAGAGCGACCGC
ACGGAGTTGGGATTTACCAGTTTCATCCGAACCCTCCGGGAGGGAGACCTCAAGAGACTAGAGAAAAGGA
GGGAAGAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208279 protein sequence
Red=Cloning site Green=Tags(s)

MARAWGLLLAIGVVLPTWLSSTKVSSLIERISDPKDLKKLLRTRNNVLVLYSESEVAAESHLKLLSTVAQ
AVKGQGTVCWVDCGDAESRKLCKKMKVDLSPKDKKIELFHYQDGAFHMQYDRAVTLKSIVAFLKDPKGPP
LWEEDPGAKDVVHIDSEKDFRRLLKREEKPLLMMFYAPWCSMCKRIMPHFQKAATQVRGHIVLAGMNVYP
SEFENIKEEYNVRGYPTICYFEKGRFLFPYENYGSTAEDIVEWLKNPLPPQPQVPETPWADEGGSVYHLT
DEDFDQFVKEHSSVLVMFHAPWCGHCKKMKPEFESAAEVLHGDAESSGVLAAVDATVNEALAGRFHISAF
PTLKYFKNGEQQAVPALRTKKKFIEWMQNPEAPPPPEPTWEEQQTSVLHLVGDNFRDTLKKKKHTLVMFY
APWCPHCKKVIPHFTATADAFKEDRKIACAAVDCVKDKNQDLCQQEAVKAYPTFHYYHYGKLVEKYESDR
TELGFTSFIRTLREGDLKRLEKRREEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028295
ORF Size 1551 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028295.1, NP_082571.1
RefSeq Size 1823 bp
RefSeq ORF 1554 bp
Locus ID 72599
UniProt ID Q921X9
Cytogenetics 16 B3
MW 59.3 kDa
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Documents
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"NM_028295" in other vectors (4)
SKU Description Size Price
MC203725 Pdia5 (untagged) - Mouse protein disulfide isomerase associated 5 (Pdia5), (10ug) 10 ug
$482.00
MG208279 Pdia5 (tGFP-tagged) - Mouse protein disulfide isomerase associated 5 (Pdia5) 10 ug
$682.00
MR208279L3 Lenti ORF clone of Pdia5 (Myc-DDK-tagged) - Mouse protein disulfide isomerase associated 5 (Pdia5) 10 ug
$782.00
MR208279L4 Lenti ORF clone of Pdia5 (mGFP-tagged) - Mouse protein disulfide isomerase associated 5 (Pdia5) 10 ug
$782.00

Plasmid Map for MR208279

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208279
Circular map for MR208279

file-alt Citations

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