Ttc8 (NM_198311) Mouse Tagged ORF Clone

SKU MR208262

Ttc8 (Myc-DDK-tagged) - Mouse tetratricopeptide repeat domain 8 (Ttc8), transcript variant 2

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ttc8
• Synonyms: 0610012F22Rik; AV001447; BBS8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208262 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCTCAGAGATGGAGCCACTGCTCCGGGCCTGGAGCTATTTTAGACGCAGGAAGTTCCAGCTCTGCG; CCGATCTATGCACGCAGATGCTGGAGAAATCCCCTTATGATCAGGAACCAGCTCCTGATTTGCCAGTGTC; TCAGGCGGCTTGGATTTTGAAAGCACGGGCGCTGACAGAGATGGTGTACATAGATGAAATTGATGTGGAC; CAGGAAGGAATTGCTGAAATGATCCTGGATGAAAATGCTATTGCTCAGGTCCCACGCCCTGGCACATCTT; TGAAGCTCCCTGGCACTAACCAAACAGGAGGACCGACTCAAGCTGTCAGGCCAATCACTCAAGCTGGAAG; ACCCATAACAGGTTTCCTTAGGCCCAGCACACAGAGTGGAAGACCAGGTACAATGGAACAGGCCATCAGA; ACACCCAGAACTGCCTACACAGCACGACCTATCACTAGCTCATCTGGAAGATTCGTCAGACTGGGGACTG; CTTCCATGCTTACAAGTCCCGATGGACCATTTATAAATTTATCTAGACTGAATTTAACAAAATATTCCCA; GAAGCCTAAGTTGGCAAAGGCTCTATTTGAATATATTCTCCACCATGAAAACGATGTTAAGATGGCTTTG; GATTTGGCGTCCCTTTCCACGGAGTATTCTCAGTACAAGGACTGGTGGTGGAAGGTACAGATTGGAAAAT; GTTACTACAGGTTAGGAATGTACCGTGAAGCAGAGAAACAGTTTAAATCAGCCTTGAAGCAGCAGGAAAT; GGTAGACACATTTCTCTATCTGGCAAAAGTTTATATCATATTGGATCAACCTGTGACTGCTTTAAATCTA; TTTAAACAAGGCTTAGATAAGTTTCCAGGAGAGGTGACCCTGCTTTGTGGAATTGCCAGGATCTATGAGG; AAATGAATAACAGTTCATCTGCAGCAGAGTACTACAAAGAAGTTCTAAAACAAGACAATACTCACGTGGA; AGCCATCGCCTGCATTGGAAGCAACCACTTTTATTCTGACCAGCCAGAGGTTGCCCTTCGGTTTTACAGG; CGCCTCTTGCAGATGGGAGTTTATAACTGCCAGCTTTTCAACAACCTGGGCCTGTGCTGCTTCTATGCCC; AGCAGTACGATATGACCCTGACCTCGTTTGAACGTGCCCTTTCCCTGGCTGAAAATGAAGAAGAGGCAGC; TGATGTCTGGTACAACCTGGGGCACATAGCTGTGGGAATCGGAGATACAAACTTGGCCCACCAATGCTTC; AGGCTGGCCCTGGTCCACAACAACCACCATGCTGAAGCCTACAACAACCTGGCAGTGCTGGAGATGCGGA; AGGGTCACGTTGAACAGGCACGCGCGCTCTTACAAACCGCATCGTCTTTGGCACCCCACATGTATGAGCC; ACACTTTAATTTCGCAACAGTCTCTGATAAGATTGGAGATCTTCAGAGAAGCTACGTTGCTGCTCAGAAG; TCTGAAGTGGCGTTTCCTGAACACGTGGACACACAGCACTTAATTAAGCAGCTGAAGCAGCACTTTGCTA; TGCTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208262 protein sequence; Red=Cloning site Green=Tags(s); ; MGSEMEPLLRAWSYFRRRKFQLCADLCTQMLEKSPYDQEPAPDLPVSQAAWILKARALTEMVYIDEIDVD; QEGIAEMILDENAIAQVPRPGTSLKLPGTNQTGGPTQAVRPITQAGRPITGFLRPSTQSGRPGTMEQAIR; TPRTAYTARPITSSSGRFVRLGTASMLTSPDGPFINLSRLNLTKYSQKPKLAKALFEYILHHENDVKMAL; DLASLSTEYSQYKDWWWKVQIGKCYYRLGMYREAEKQFKSALKQQEMVDTFLYLAKVYIILDQPVTALNL; FKQGLDKFPGEVTLLCGIARIYEEMNNSSSAAEYYKEVLKQDNTHVEAIACIGSNHFYSDQPEVALRFYR; RLLQMGVYNCQLFNNLGLCCFYAQQYDMTLTSFERALSLAENEEEAADVWYNLGHIAVGIGDTNLAHQCF; RLALVHNNHHAEAYNNLAVLEMRKGHVEQARALLQTASSLAPHMYEPHFNFATVSDKIGDLQRSYVAAQK; SEVAFPEHVDTQHLIKQLKQHFAML; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198311
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_198311.2
• RefSeq Size: 2287 bp
• RefSeq ORF: 1548 bp
• Locus ID: 76260
• UniProt ID: Q8VD72
• Cytogenetics: 12 E
• MW: 58.4 kDa
• Summary: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization (By similarity).[UniProtKB/Swiss-Prot Function]