Midn (NM_021565) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR208176
Midn (Myc-DDK-tagged) - Mouse midnolin (Midn)
  $709.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Midn
Synonyms 3000003C15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208176 representing NM_021565
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCGCAGCCCGGCGGTGCCCGGAGCTGCCGGCGCGGGGCCCCCGGCGGCGCCTGCGAGCTGAACA
CGGCCACGGAGTCGGCCGCACCCATGAGCCTGGCGATCCACAGCACTACGGGGACCCGCTACGACCTGTC
GGTGCCCCACGACGAGACCGTGGAAGGGCTGCGCAAAAGGTTGTCCCAACGCCTCAAAGTACCCAAGGAA
CGCCTGGCGCTGCTTCACAAAGACACCCGGCTCAGTTCGGGGAAGCTGCAGGAATTCGGCGTGGGGGATG
GGAGCAAGTTGACGCTCGTGCCCACGGTGGAAGCTGGCCTCATGTCCCAGGCCTCGAGGCCGGAACAGTC
CGTTATGCAAGCCTTGGAAAGTTTGACCGAGACCCAGCCCCCAGCGACACCCGGGCCAGGCCGGGCTGCC
GGAGGAGGCTTCCGGAAATACAGATTGATTTTATTTAAGCGTCCGTGGCACCGACAGGGACCCCAGAGCC
CAGAGAGGGGCGGCGAGAGGCCCCAGGTCAGTGACTTTCTGTCAGGCCGCTCGCCTTTGACCCTGGCCCT
GCGAGTCGGGGATCACATGATGTTTGTGCAGTTGCAACTGGCAGCCCAGCATGCCCCACTCCAGCACCGC
CATGTGCTTGCTGCTGCCGCAGCAGCAGCCGCCGCCGCCCGGGGAGACTCCAGTGTAGCCACCCCAGTGT
CTTCACCCTGTAGGCCGGTGTCCAGTGCCGCCCGTGTACCCCCAGTATCCAGCAGCCCTTCCTCACCTGT
ATCCCCCTCACCTGTCACTGCTGGCTCCTTCCGATCCCACGCAGCCTCCACAACCTGTCCTGAGCAGATG
GACTGTTCTCCACCCGCCAGCAGCAGCTCCACATCCACCCCAGGCAGCAGCCCCACCCCTCGATCCCGCA
AACCTGGTGCGGTCATTGAGAGCTTCGTGAACCATGCTCCAGGGGTCTTCTCAGGGACCTTCTCTGGCAC
ACTACACCCCAACTGCCAGGACAGCAGTGGGCGGCCTCGGCGTGACATCGGCACCATTCTGCAGATACTC
AATGACCTCCTAAGTGCCACGAGGCACTACCAGGGCATGCCACCCTCACTGACCCAGCTCCGCTGCCATG
CTCAGTGCTCACCTGCCTCACCAGCCCCTGACCTCACCCCCAAAACTACCTCCTGTGAAAAGCTGGCAGC
CACCTCCTCCACATCCCTGCTCCAGGGCCAGAGCCAGATTCGAATGTGCAAGCCCCCTGGAGACCGTCTT
CGACAGACAGAGAACCGTGCCACACGTTGCAAAGTGGAACGCCTCCAGCTCCTGTTGCAGCAGAAGCGCC
TGCGCAGGAAGGCACGGCGGGACGCCCGGGGGCCTTACCACTGGACCCCCAGCCGCAAAGCTGGCCGTAG
CGACAGCAGCAGCAGTGGGGGTGGAGGTGGCCCCAGCGAGGCCACAGGCTTGGGCCTCGACTTCGAGGAC
TCCGTTTGGAAGCCTGAAGTCAACCCGGACATCCAGTCCGAGTTTGTGGTGGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR208176 representing NM_021565
Red=Cloning site Green=Tags(s)

MEPQPGGARSCRRGAPGGACELNTATESAAPMSLAIHSTTGTRYDLSVPHDETVEGLRKRLSQRLKVPKE
RLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQPPATPGPGRAA
GGGFRKYRLILFKRPWHRQGPQSPERGGERPQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHR
HVLAAAAAAAAAARGDSSVATPVSSPCRPVSSAARVPPVSSSPSSPVSPSPVTAGSFRSHAASTTCPEQM
DCSPPASSSSTSTPGSSPTPRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQIL
NDLLSATRHYQGMPPSLTQLRCHAQCSPASPAPDLTPKTTSCEKLAATSSTSLLQGQSQIRMCKPPGDRL
RQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWTPSRKAGRSDSSSSGGGGGPSEATGLGLDFED
SVWKPEVNPDIQSEFVVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021565
ORF Size 1524 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021565.2, NP_067540.1
RefSeq Size 3747 bp
RefSeq ORF 1527 bp
Locus ID 59090
UniProt ID Q3TPJ7
Cytogenetics 10 C1
MW 53.9 kDa
Summary This gene encodes a protein that contains an ubiquitin-like domain. This protein may be involved in the regulation of brain development as inferred by its high expression level in the embryonic midbrain. This protein has been found to negatively regulate glucokinase activity and insulin secretion in pancreatic beta cells. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2015
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Documents
Resources
"NM_021565" in other vectors (4)
SKU Description Size Price
MC217169 Midn (untagged) - Mouse midnolin (Midn), (10ug) 10 ug
$758.00
MG208176 Midn (tGFP-tagged) - Mouse midnolin (Midn) 10 ug
$909.00
MR208176L3 Lenti ORF clone of Midn (Myc-DDK-tagged) - Mouse midnolin (Midn) 10 ug
$1,009.00
MR208176L4 Lenti ORF clone of Midn (mGFP-tagged) - Mouse midnolin (Midn) 10 ug
$1,009.00

Plasmid Map for MR208176

Download sequence for

Cloning scheme for MR208176

Circular map for MR208176

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