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Cnot11 (NM_028043) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208127
Cnot11 (Myc-DDK-tagged) - Mouse DNA segment, Chr 1, Brigham & Women's Genetics 0212 expressed (D1Bwg0212e)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $470.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cnot11
Synonyms 2410015L18Rik; C40; D1Bwg0212e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208127 representing NM_028043
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCGGCGGCGGGGCGAGCACGGCGTCTGGCCGGCTTCTCTCCTCAGCGGATCCCAGAGGGGCGCGGG
AGGCAGCGGCGTTCCGGAGCGGCCCGGCGGGCTCGAGCGGAGGCCGAGGCGGAGCAGGCGGCCCTGGGCC
AGGGATTGGAGGCCCCGCGGGCAGGATGAGCCTGACCCCGAAGGAGCTCTCGAGCCTGCTGAGCATCATC
TCGGAGGAGGCGGGCGGCGGCAGCACCTTCGAGGGCCTGTCCACCGCCTTCCACCACTACTTCAGCAAGG
CCGACCACTTCCGCCTGGGCTCGGTGCTGGTGATGCTGCTGCAGCAGCCGGACCTGCTGCCTAGCGCGGC
GCAGCGCCTCACGGCGCTCTACCTGCTCTGGGAGATGTACCGCACCGAGCCGCTCGCCGCCAACCCGTTC
GCGGCCAGCTTCGCGCACCTGCTCAACCCGGCGCCGCCCGCCCGCGGCGGCCAGGAGCCCGACCGGCCCC
CGCTCTCAGGGTTCCTGCCTCCTATAACTCCACCAGAAAAGTTTTTCCTCTCCCAGCTCATGCTGGCACC
CCCAAGGGAACTGTTCAAAAAGACACCTCGCCAGATTGCCTTGATGGACGTTGGAAACATGGGACAGTCT
GTGGACATTAGCGGCCTTCAGTTAGCCCTGGCAGAGCGTCAGTCTGAGCTGCCAACCCAGAGCAAAGCCA
GCTTCCCAAGCATCCTCAGTGACCCAGACCCGGACTCGTCCAACTCTGGATTTGACAGCTCAGTTGCCTC
TCGGATCACAGAGTCTTTAGTCAGTGGCCCAAAGCCACCTATTGAAAGCCACTTTCGACCAGAGTTTATC
CGCCCACCACCCCCTCTCCACATCTGTGAGGATGAACTGGCCTGGCTAAATCCCACAGAACCCGAGCATG
CCATCCAGTGGGACAGATCGATGTGTGTGAAGAATAGCACTGGTGTGGAGATCAAGCGGATCATGGCGAA
AGCCTTCAAAAGCCCCCTGTCGTCTCCCCAACAGACACAGCTCCTCGGTGAGTTGGAAAAAGATCCCAAA
CTTGTTTATCACATTGGCCTTACTCCAGCGAAACTTCCAGATCTAGTGGAGAACAACCCTTTAGTCGCTA
TAGAAATGCTGCTGAAACTGATGCAGTCAAGCCAGATCACGGAGTATTTCTCTGTCCTGGTCAACATGGA
CATGTCCTTACATTCAATGGAAGTTGTAAATCGACTAACTACAGCTGTTGACCTACCTCCTGAATTTATT
CACCTATACATATCCAATTGCATTTCTACTTGTGAACAGATTAAGGATAAATATATGCAGAATCGTTTGG
TGCGTCTTGTGTGTGTGTTTCTCCAGTCTTTGATCCGTAACAAAATTATTAACGTGCAGGACTTGTTTAT
AGAAGTGCAGGCCTTCTGCATTGAATTTAGTAGGATACGGGAAGCTGCAGGCCTATTCCGGTTGCTGAAG
ACACTGGATACTGGGGAAACGCCTTCAGAGACCAAAATATCAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208127 representing NM_028043
Red=Cloning site Green=Tags(s)

MPGGGASTASGRLLSSADPRGAREAAAFRSGPAGSSGGRGGAGGPGPGIGGPAGRMSLTPKELSSLLSII
SEEAGGGSTFEGLSTAFHHYFSKADHFRLGSVLVMLLQQPDLLPSAAQRLTALYLLWEMYRTEPLAANPF
AASFAHLLNPAPPARGGQEPDRPPLSGFLPPITPPEKFFLSQLMLAPPRELFKKTPRQIALMDVGNMGQS
VDISGLQLALAERQSELPTQSKASFPSILSDPDPDSSNSGFDSSVASRITESLVSGPKPPIESHFRPEFI
RPPPPLHICEDELAWLNPTEPEHAIQWDRSMCVKNSTGVEIKRIMAKAFKSPLSSPQQTQLLGELEKDPK
LVYHIGLTPAKLPDLVENNPLVAIEMLLKLMQSSQITEYFSVLVNMDMSLHSMEVVNRLTTAVDLPPEFI
HLYISNCISTCEQIKDKYMQNRLVRLVCVFLQSLIRNKIINVQDLFIEVQAFCIEFSRIREAAGLFRLLK
TLDTGETPSETKISK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028043
ORF Size 1515 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028043.3
RefSeq Size 3205 bp
RefSeq ORF 1518 bp
Locus ID 52846
UniProt ID Q9CWN7
Cytogenetics 1 18.31 cM
MW 55 kDa
Summary Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is required for the association of CNOT10 with the CCR4-NOT complex. Seems not to be required for complex deadenylase function (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028043" in other vectors (4)
SKU Description Size Price
MC204896 Cnot11 (untagged) - Mouse DNA segment, Chr 1, Brigham & Women's Genetics 0212 expressed (D1Bwg0212e), (10ug) 10 ug
$471.00
MG208127 Cnot11 (tGFP-tagged) - Mouse DNA segment, Chr 1, Brigham & Women's Genetics 0212 expressed (D1Bwg0212e) 10 ug
$670.00
MR208127L3 Lenti ORF clone of Cnot11 (Myc-DDK-tagged) - Mouse DNA segment, Chr 1, Brigham & Women's Genetics 0212 expressed (D1Bwg0212e) 10 ug
$770.00
MR208127L4 Lenti ORF clone of Cnot11 (mGFP-tagged) - Mouse DNA segment, Chr 1, Brigham & Women's Genetics 0212 expressed (D1Bwg0212e) 10 ug
$770.00

Plasmid Map for MR208127

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208127
Circular map for MR208127

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