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Shmt2 (NM_028230) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208109
Shmt2 (Myc-DDK-tagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $470.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Shmt2 or NM_028230"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Shmt2
Synonyms 2700043D08Rik; AA408223; AA986903
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208109 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTATCCTTCTCTTTGCTTCGGACCACTCGGCCTCTGCAGAGATGTGGCCAGCTGGTCTGCATGGCTG
CCCGGGCCCAGCACAGCAAGGTGGCCCAGACGCAGGCTGGGGAAGCAACTGGAGGTTGGACGGGCCAGGA
GAGTTTATCAGACAGTGACCCTGAGATGTGGGAGCTTCTGCAGAGGGAGAAGGACAGACAGTGTCGCGGC
CTGGAGCTCATCGCCTCAGAGAACTTCTGCAGCCGAGCTGCGCTGGAGGCCCTGGGGTCCTGTCTCAACA
ACAAGTACTCGGAGGGTTACCCTGGCAAGAGATACTACGGAGGAGCGGAAGTGGTGGACGAGATCGAGCT
GCTCTGCCAGCGCCGGGCCTTGGAAGCCTTTGACCTGGATCCGGCACAGTGGGGAGTCAATGTGCAGCCA
TACTCAGGGTCCCCAGCCAATCTGGCTGCCTATACGGCCCTTCTGCAGCCTCATGATCGAATCATGGGGT
TGGACCTGCCCGATGGGGGCCATCTCACCCATGGCTACATGTCTGATGTCAAGCGGATCTCCGCCACATC
CATTTTCTTCGAGTCTATGCCCTATAAGCTCAATCCCCAAACTGGCCTCATCGACTACGACCAGCTGGCG
CTGACCGCTCGGCTTTTCCGACCGCGGCTCATCATAGCTGGCACGAGTGCCTATGCCCGCCTCATTGACT
ATGCACGCATGAAAGAGGTCTGTGATGAGGTCAGGGCACACCTGCTGGCAGACATGGCCCACATCAGTGG
CCTGGTGGCTGCCAAGGTGATCCCCTCCCCTTTCAAGTACGCGGATGTTGTTACCACCACCACTCACAAG
ACACTGCGAGGGGCCAGGTCAGGGCTCATCTTCTACCGGAAGGGAGTACGAACCGTAGACCCCAAGACTG
GCAAAGAGATCCCTTATACCTTTGAGGACCGAATCAACTTCGCTGTGTTCCCATCCCTACAGGGTGGCCC
CCACAACCACGCCATTGCTGCAGTAGCCGTGGCTCTCAAGCAGGCCTGCACCCCTATGTTCCGCGAGTAC
TCCTTACAAGTGCTGAGGAACGCCCAGGCCATGGCTGATGCCCTGCTCAAGCGAGGATACTCGCTGGTGT
CTGGTGGCACTGACACCCACCTGGTGCTGGTGGACCTGCGGCCCAAGGGCCTGGATGGAGCCCGAGCCGA
ACGCGTGTTGGAACTTGTCTCCATCACAGCCAACAAGAACACCTGTCCTGGAGACCGGAGCGCCATTACT
CCGGGGGGCTTGAGGCTTGGGGCCCCCGCGTTGACCTCTCGCCAGTTCCGTGAGGACGACTTCCGTAGAG
TCGTCGATTTTATCGATGAGGGAGTCAACATTGGCTTGGAGGTGAAGCGCAAGACTGCCAAGCTCCAGGA
TTTCAAATCCTTCCTGCTCAAAGACCCAGAGACAAGCCAGCGTTTGGCCAACCTCCGGCAACAGGTGGAA
CAGTTTGCCAGGGGCTTCCCGATGCCTGGATTTGATGAACGT

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208109 protein sequence
Red=Cloning site Green=Tags(s)

MVSFSLLRTTRPLQRCGQLVCMAARAQHSKVAQTQAGEATGGWTGQESLSDSDPEMWELLQREKDRQCRG
LELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQP
YSGSPANLAAYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPQTGLIDYDQLA
LTARLFRPRLIIAGTSAYARLIDYARMKEVCDEVRAHLLADMAHISGLVAAKVIPSPFKYADVVTTTTHK
TLRGARSGLIFYRKGVRTVDPKTGKEIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREY
SLQVLRNAQAMADALLKRGYSLVSGGTDTHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAIT
PGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKRKTAKLQDFKSFLLKDPETSQRLANLRQQVE
QFARGFPMPGFDER

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_028230
ORF Size 1512 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028230.1, NM_028230.2, NM_028230.3, NM_028230.4, NP_082506.1
RefSeq Size 2305 bp
RefSeq ORF 1515 bp
Locus ID 108037
UniProt ID Q9CZN7
Cytogenetics 10 D3
MW 55.8 kDa
Summary Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (By similarity). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (By similarity). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (By similarity). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29452640). Associates with mitochondrial DNA (By similarity). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (By similarity).UniProtKB/Swiss-Prot Function
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"NM_028230" in other vectors (4)
SKU Description Size Price
MC202532 Shmt2 (untagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$470.00
MG208109 Shmt2 (tGFP-tagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2) 10 ug
$670.00
MR208109L3 Lenti ORF clone of Shmt2 (Myc-DDK-tagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), nuclear gene encoding mitochondrial protein 10 ug
$770.00
MR208109L4 Lenti ORF clone of Shmt2 (mGFP-tagged) - Mouse serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), nuclear gene encoding mitochondrial protein 10 ug
$770.00

Plasmid Map for MR208109

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file-download TXT (FASTA format)

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Cloning scheme for MR208109
Circular map for MR208109

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