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Slc2a5 (NM_019741) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR208045
Slc2a5 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 5 (Slc2a5)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $467.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc2a5
Synonyms AI526984; Glut5; Slc5a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR208045 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAAAAACATCAAGAGGAGACAGGGGAGCTGACATTGGTCCTTGCTCTGGCAACGCTCATCGCTG
CCTTTGGCTCATCCTTCCAATATGGGTACAACGTAGCTGCCGTCAACTCTCCCTCAGAGTTCATGCAGCA
GTTTTACAATGACACCTACTACGACAGAAATGAGGAGAATATTGAGTCCTTCACCTTGACGCTGCTGTGG
TCCCTGACGGTGTCCATGTTCCCCTTTGGGGGCTTTATAGGCTCTCTCATGGTTGGAACCTTGGTGAATA
AACTGGGCAGAAAAGGGGCCCTGCTGTTCAACAACATATTCTCCATCCTGCCGGCCATCTTAATGGGCTG
CAGCCAAATTGCCCAATCGTTTGAGCTAATAATTATTTCCAGACTTCTGGTTGGAATCTGTGCAGGTATC
TCTTCCAACGTGGTCCCTATGTACTTAGGGGAGCTGGCCCCGAAAAACCTACGAGGGGCTCTGGGAGTGG
TCCCGCAACTCTTCATCACTGTCGGCATCCTTGTGGCCCAGCTGTTTGGCCTTCGGAGTCTCTTGGCAAA
TGAGGATGGCTGGCCAGTTCTCCTGGGTCTAACTGGAGTCCCCGCAGGCCTTCAGCTCCTCCTCCTCCCG
TTCTTTCCCGAGAGCCCCCGCTACCTGCTGATCCAGAAGAAAGATGAAGCAGCTGCTGAGAGAGCCCTCC
AGACCCTCCGAGGCTGGAAAGACGTGCACCTAGAGATGGAGGAGATCCGGAAGGAGGATGAGGCTGAGAA
GGCGGCGGGCTTCATCTCTGTGTGGAAGTTGTTCACTATGCAGTCTCTCCGTTGGCAACTCATCTCCATG
ATTGTCCTCATGGCTGGCCAGCAGCTGTCGGGAGTGAACGCGATCTACTACTACGCCGATCAGATCTACC
TCAGCGCAGGCGTGAAAAGCGACGACGTCCAGTATGTGACAGCCGGGACTGGGGCCGTCAATGTGTTCAT
GACCATCCTCACGATCTTTGTGGTAGAGCTTTGGGGACGGCGATTCCTACTCCTCGTCGGCTTCTCCACC
TGCCTCATAGCCTGCTTAGTGCTGACGGCCGCACTGGCGCTGCAGAACACCATCTCCTGGATGCCCTATA
TCAGCATTGTCTGTGTCATTGTCTACGTCATAGGACACGCCTTAGGACCCAGCCCCATCCCTGCCCTGCT
CATCACTGAGATCTTCCTGCAGTCCTCCCGGCCAGCCGCCTACATGATCGGAGGCAGTGTCCACTGGCTC
TCTAACTTCACTGTGGGGCTCATCTTCCCCTTCATTCAAATGGGCCTCGGTCCCTACAGCTTCATTATCT
TTGCAACCATCTGTTTCCTCACCACCATCTACATCTTCATGGTCGTCCCAGAGACCAAGGGCAGGACATT
CATTGAGATCAACCAGATTTTTACCATGAAGAACAAGGTGTCAGACGTATATCCGAAGAAGGAGGAGGAG
CTTGGCGCCCTCCCACACGCCATCTTGGAGCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR208045 protein sequence
Red=Cloning site Green=Tags(s)

MEEKHQEETGELTLVLALATLIAAFGSSFQYGYNVAAVNSPSEFMQQFYNDTYYDRNEENIESFTLTLLW
SLTVSMFPFGGFIGSLMVGTLVNKLGRKGALLFNNIFSILPAILMGCSQIAQSFELIIISRLLVGICAGI
SSNVVPMYLGELAPKNLRGALGVVPQLFITVGILVAQLFGLRSLLANEDGWPVLLGLTGVPAGLQLLLLP
FFPESPRYLLIQKKDEAAAERALQTLRGWKDVHLEMEEIRKEDEAEKAAGFISVWKLFTMQSLRWQLISM
IVLMAGQQLSGVNAIYYYADQIYLSAGVKSDDVQYVTAGTGAVNVFMTILTIFVVELWGRRFLLLVGFST
CLIACLVLTAALALQNTISWMPYISIVCVIVYVIGHALGPSPIPALLITEIFLQSSRPAAYMIGGSVHWL
SNFTVGLIFPFIQMGLGPYSFIIFATICFLTTIYIFMVVPETKGRTFIEINQIFTMKNKVSDVYPKKEEE
LGALPHAILEQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019741
ORF Size 1503 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019741.3
RefSeq Size 3096 bp
RefSeq ORF 1506 bp
Locus ID 56485
UniProt ID Q9WV38
Cytogenetics 4 E2
MW 55.4 kDa
Summary Functions as a fructose transporter that has only low activity with other monosaccharides (PubMed:12031501, PubMed:19091748). Can mediate the uptake of deoxyglucose, but with low efficiency (By similarity). Essential for fructose uptake in the small intestine (PubMed:19091748, PubMed:26071406). Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose (PubMed:19091748). Required for the development of high blood pressure in response to high dietary fructose intake (PubMed:19091748).UniProtKB/Swiss-Prot Function
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Documents
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"NM_019741" in other vectors (4)
SKU Description Size Price
MC204180 Slc2a5 (untagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 5 (Slc2a5), (10ug) 10 ug
$467.00
MG208045 Slc2a5 (tGFP-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 5 (Slc2a5) 10 ug
$667.00
MR208045L3 Lenti ORF clone of Slc2a5 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 5 (Slc2a5) 10 ug
$767.00
MR208045L4 Lenti ORF clone of Slc2a5 (mGFP-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 5 (Slc2a5) 10 ug
$767.00

Plasmid Map for MR208045

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR208045
Circular map for MR208045

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