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Slc19a2 (NM_054087) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207986
Slc19a2 (Myc-DDK-tagged) - Mouse solute carrier family 19 (thiamine transporter), member 2 (Slc19a2)
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc19a2
Synonyms AV276020; AW322295; DDA1; THTR1; TRMA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207986 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGTGCCAGCCAGGGTGTCCAGACGAGCGGCGGCGGCGGCGGCCAGGATGCTTCTGCGTACTGCCC
GCGTCCCTCGCGAGTGCTGGTTCCTGCCGACCGCGCTGCTCTGCGCCTACGGCTTCTTCGCAAACCTCCG
GCCGTCGGAGCCGTTCCTCACGCCCTACCTTCTGGGACCCGACAAGAACTTGACCGAGAGACAGGTCTAC
AATGAAATTTATCCGGTGTGGACGTACTCTTACCTGCTGCTGCTGTTTCCCGTGTTCCTTGCCACAGACT
ACCTCCGGTACAAGCCTGTCATCCTGCTGCAGGGACTCAGCCTGATTGTGACGTGGTTCATGCTGCTCTA
TGCCCAGGGACTGCTGGCCATTCAGTTCTTGGAATTCTTCTACGGCATCGCCACAGCCACCGAAATCGCC
TACTACTCCTATATCTATACTGTGGTGGACCTGGGCATGTACCAGAAAGTCACAAGCTACTGTAGAAGTG
CCACCTTGGTGGGCTTTACAGTGGGCTCCGTCCTAGGGCAGATCCTCGTCTCCGTGGTGGGCTGGTCACT
GTTCAGCTTGAACGTCATCTCCCTCACCTGTGTTTCTGTGGCTTTTGCTGTGGCCTGGTTTCTGCCTATG
CCACAGAAGAGCCTCTTCTTTCACCACATTCCTTCCTCCTGTCATGGAGTGAACGGCCTCAAGGTACAAA
ACGGTGGCATCGTTACTGATACCCCAGCAGCTAACCATCTTCCTGGATGGGAGGACATTGAGTCAAAAAT
CCCTCTAAATTTAGATGAGCCTCCGGTGGAAGAACCGGAGGAGCCCAAGCCAGACCGGCTGCGGGTGTTC
AGAGTCCTGTGGAATGACTTCCTGATGTGTTATTCCTCCCGCCCTCTGCTCTGCTGGTCCGTGTGGTGGG
CCCTGTCCACCTGCGGCTATTTCCAAGTGGTGAACTACGCGCAGGGATTGTGGGAGAAGGTGATGCCTTC
TCAGAATGCTGACATCTACAATGGCGGTGTGGAGGCCGTTTCAACCTTGCTGGGTGCTAGTGCTGTGTTT
GCAGTTGGCTATATAAAGCTATCTTGGTCAACTTGGGGAGAAATGACGTTGTTCCTGTGTTCTCTCCTGA
TTGCTGCTGCAGTGTATGTCATGGACACTGTGCAGAGCATCTGGGTGTGCTATGCATCCTATGTTGTCTT
CAGAATCATCTACATGGTACTCATCACCATAGCAACTTTCCAGATTGCTGCGAACCTCAGCATGGAACGT
TACGCCCTTGTGTTCGGCGTGAACACCTTCATTGCCCTGGCATTGCAGACTCTGCTCACTCTGATTGTCG
TGGATGCCAGGGGCCTTGGCTTATGTATCACCACTCAGTTCCTGATTTACGCCAGTTACTTCGCAGCCAT
CTCTGTGGTTTTCCTGGCGAATGGCATAGTCAGTATTATAAAGAAATGCAGAAAGCAGGAAGATCCCAGC
TCCAGCCCCCAAGCCTCCACGTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207986 protein sequence
Red=Cloning site Green=Tags(s)

MDVPARVSRRAAAAAARMLLRTARVPRECWFLPTALLCAYGFFANLRPSEPFLTPYLLGPDKNLTERQVY
NEIYPVWTYSYLLLLFPVFLATDYLRYKPVILLQGLSLIVTWFMLLYAQGLLAIQFLEFFYGIATATEIA
YYSYIYTVVDLGMYQKVTSYCRSATLVGFTVGSVLGQILVSVVGWSLFSLNVISLTCVSVAFAVAWFLPM
PQKSLFFHHIPSSCHGVNGLKVQNGGIVTDTPAANHLPGWEDIESKIPLNLDEPPVEEPEEPKPDRLRVF
RVLWNDFLMCYSSRPLLCWSVWWALSTCGYFQVVNYAQGLWEKVMPSQNADIYNGGVEAVSTLLGASAVF
AVGYIKLSWSTWGEMTLFLCSLLIAAAVYVMDTVQSIWVCYASYVVFRIIYMVLITIATFQIAANLSMER
YALVFGVNTFIALALQTLLTLIVVDARGLGLCITTQFLIYASYFAAISVVFLANGIVSIIKKCRKQEDPS
SSPQASTS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_054087
ORF Size 1494 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_054087.3, NP_473428.1
RefSeq Size 3571 bp
RefSeq ORF 1497 bp
Locus ID 116914
UniProt ID Q9EQN9
Cytogenetics 1 71.56 cM
MW 55.7 kDa
Summary High-affinity transporter for the intake of thiamine.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_054087" in other vectors (3)
SKU Description Size Price
MC216939 Slc19a2 (untagged) - Mouse solute carrier family 19 (thiamine transporter), member 2 (Slc19a2), (10ug) 10 ug
$503.00
MR207986L3 Lenti ORF clone of Slc19a2 (Myc-DDK-tagged) - Mouse solute carrier family 19 (thiamine transporter), member 2 (Slc19a2) 10 ug
$757.00
MR207986L4 Lenti ORF clone of Slc19a2 (mGFP-tagged) - Mouse solute carrier family 19 (thiamine transporter), member 2 (Slc19a2) 10 ug
$757.00

Plasmid Map for MR207986

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207986
Circular map for MR207986

file-alt Citations

Post-transcriptional regulation of thiamin transporter-1 (THTR-1) expression by microRNA-200a-3p in pancreatic acinar cells
Ramamoorthy, Anandam, Yasujima et al
Am. J. Physiol. Gastrointest. Liver Physiol. (2020) 319 (3), G323-G332 DOI: 10.1152/ajpgi.00178.2020
Cited by 7
AI Summary: OriGene’s mouse and human SLC19A2 plasmid constructs (MR207986 and RC216515) were used to overexpress THTR-1 in cell lines, enabling validation of antibody specificity in the study.
Snippet: antibodies was validated by overexpressing the mouse and human SLC19A2 carrying plasmid construct 214 (MR207986 and RC216515;OriGene Technologies, MD) in PAC 266-6 and human-derived intestinal 215 epithelial Caco-2 cells (see Fig. 3B. ii and Fig. 4C;- antibodies was validated by overexpressing the mouse and human SLC19A2 carrying plasmid construct 214 (MR207986 and RC216515
Showing 1-1 of 1 papers.
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