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5730410E15Rik (BC048945) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207983
5730410E15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730410E15 gene (cDNA clone MGC:57027 IMAGE:6467420)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "5730410E15Rik or BC048945"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 5730410E15Rik
Synonyms Golsyn
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207983 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACGACAGGAAACAAGAGAGCCTCTTTCTCACGCAACCGAGGCCCTCATGGGCGGAGCAATGGAG
CATCATCCCACAAGTCTGGCAGCAGCCCACCATCCCCAAGGGAAAAAGACCTTGTGTCTATGCTGTGCAG
GAATCCACTGAGCCCCAGTAACATCCACCCTAGTTACGCCCCTTCTTCTCCAAGTAGCAGCAACTCCGGC
TCCTACAAAGGAAGTGATTGTAGTCCAGTCATGAGAAGGTCTGGAAGATACATGTCTTGTGGCGAAAATC
ACGGTGTCAAACCCCCAAATCCAGAACAGTATTTGACTCCTCTGCAGCAGAAGGAGGTCACAGTGAGGCA
CCTGAGGACCAAGCTGAAGGAGTCTGAGCGCCGACTCCATGAGAGGGAATCTGAAATCATGGAGCTCAAG
TCTCAGCTGGCTCGAATGAGGGAGGACTGGATTGAGGAAGAGTGCCACAGAGTGGAGGCTCAGTTGGCAC
TCAAAGAAGCCAGAAAAGAGATTAAACAGCTCAAACAGGTCATCGAGACTATGAGGAGCAGCTTGGCTGA
TAAAGATAAAGGCATTCAGAAGTACTTTGTGGACATAAACATCCAAAACAAGAAACTGGAGTCTCTGCTT
CAAAGCATGGAGATGGCACACAATAGTTCCCTGAGGGATGAACTGTGTCTTGACTTTTCCTTTGATTCCC
CAGAGAAAAGTTTACCCCTGAGCAGCACATTTGACAAGTTGCCAGATGGGTTATCTCTGGAAGAACAGAT
AACAGAGGAAGGTGCTGACAGTGAGCTTCTGGTGGGAGACAGCATGGCTGAAGGCACAGATCTGTTAGAT
GAGATGGTGACTGCCACCACCACAGAATCCAGTGGCCTGGAGTTTGTTCATTCCACTCCAGGGCCACAAG
CCCTCAAGGCTCTCCCCTTGGTGAGCCACGAAGAGGGCATTGCGGTGATGGAGCAAGCCGTGCAGACCGA
CGTGGTGCCGTTCAGCCCTGCCATCTCAGAGCTCATTCAGAGTGTGCTAAAGCTGCAGGACTACTGTCCC
ACAAGCTCAGCATCTCCAGATGAATCTGGAGCTGACTCGATGGAAAGCTTCTCAGAATCTATCTCTGCCT
TAATGCTTGATTTAACTCCAAGAAGTCCTAACTCGGCCATCCTTCTGTCTCCTGTGGAGATCCCATTCAG
CAAGGGGGCTATGGAAGCCCATGCAAATCGCCTCATGAGAGAGCTAGATTTTGCAGCCTACACAGAAGAA
AGATTGGACAGTGTCCTCTCACTGTCCCAGGGCAGTGTTGTGAGGCAGTACTGGAGCAGCAATTTCTTGG
TGGATCTACTGGCTGTGGCTGCCCCTGTGGTACCCACTGTTCTGTGGGCATTCAGTACTCAGAGAGGGGG
TACAGATCCTGTCTACAACATCGGAGCTTTGCTCCGAGGTTGCTGTGTGGTGGCCCTACACTCACTACGC
CGCACCGCTTTCCACATGAAAACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207983 protein sequence
Red=Cloning site Green=Tags(s)

MSTTGNKRASFSRNRGPHGRSNGASSHKSGSSPPSPREKDLVSMLCRNPLSPSNIHPSYAPSSPSSSNSG
SYKGSDCSPVMRRSGRYMSCGENHGVKPPNPEQYLTPLQQKEVTVRHLRTKLKESERRLHERESEIMELK
SQLARMREDWIEEECHRVEAQLALKEARKEIKQLKQVIETMRSSLADKDKGIQKYFVDINIQNKKLESLL
QSMEMAHNSSLRDELCLDFSFDSPEKSLPLSSTFDKLPDGLSLEEQITEEGADSELLVGDSMAEGTDLLD
EMVTATTTESSGLEFVHSTPGPQALKALPLVSHEEGIAVMEQAVQTDVVPFSPAISELIQSVLKLQDYCP
TSSASPDESGADSMESFSESISALMLDLTPRSPNSAILLSPVEIPFSKGAMEAHANRLMRELDFAAYTEE
RLDSVLSLSQGSVVRQYWSSNFLVDLLAVAAPVVPTVLWAFSTQRGGTDPVYNIGALLRGCCVVALHSLR
RTAFHMKT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC048945
ORF Size 1494 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC048945, AAH48945
RefSeq Size 2679 bp
RefSeq ORF 1496 bp
Locus ID 319613
Cytogenetics 15 B3.2
MW 54.9 kDa
Summary Part of a kinesin motor-adapter complex that is critical for the anterograde axonal transport of active zone components and contributes to activity-dependent presynaptic assembly during neuronal development.UniProtKB/Swiss-Prot Function
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"BC048945" in other vectors (4)
SKU Description Size Price
MC218048 5730410E15Rik (untagged) - Mouse RIKEN cDNA 5730410E15 gene (cDNA clone MGC:57027 IMAGE:6467420), (10ug) 10 ug
$503.00
MG207983 5730410E15Rik (tGFP-tagged) - Mouse RIKEN cDNA 5730410E15 gene (cDNA clone MGC:57027 IMAGE:6467420) 10 ug
$657.00
MR207983L3 Lenti ORF clone of 5730410E15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730410E15 gene (cDNA clone MGC:57027 IMAGE:6467420) 10 ug
$757.00
MR207983L4 Lenti ORF clone of 5730410E15Rik (mGFP-tagged) - Mouse RIKEN cDNA 5730410E15 gene (cDNA clone MGC:57027 IMAGE:6467420) 10 ug
$757.00

Plasmid Map for MR207983

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207983
Circular map for MR207983

file-alt Citations

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