OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Mlycd (NM_019966) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR207886
Mlycd (Myc-DDK-tagged) - Mouse malonyl-CoA decarboxylase (Mlycd), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Mlycd or NM_019966"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mlycd
Synonyms AI324784; Mcd
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207886 representing NM_019966
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGAGGCCTCGGGCCAGGCTTGAGGGCTCGGCGCCTGCTCCCACTGCGGTCCCCGCCGCGGCCTCCGG
GGCCCCGGGGACGTCGGCTGTGCGGCGGGCTCGCGGCCAGCGCCATGGACGAGCTGCTGCGGCGAGCCGT
GCCCCCGACGCCGGCCTACGAGCTGCGCGAGAAGACGCCGGCGCCCGCCGAGGGCCAGTGCGCCGACTTC
GTGAGCTTCTACGGCGGCCTGGCCGAGGCGTCCCAGCGCGCCGAGCTGCTCGGCCGCCTGGCTCAGGGCT
TCGGCGTGGACCACGGCCAGGTGGCCGAGCAGAGCGCCGGGGTGCTGCAGCTGCGCCAGCAGGCGCGCGA
GGCGGCCGTGCTGCTGCAGGCGGAGGACCGGTTGCGCTACGCGCTCGTGCCGCGGTATCGCGGCCTCTTC
CATCACATCAGCAAGCTGGACGGCGGCGTGCGCTTCTTGGTGCAGCTGCGGGCCGATCTGCTGGAGGCGC
AGGCCCTCAAGCTGGTGGAGGGGCCGCACGTCCGGGAAATGAACGGAGTGCTCAAAAGCATGCTGTCTGA
GTGGTTCTCCTCCGGCTTCCTGAACCTGGAGCGGGTCACCTGGCACTCACCCTGTGAGGTACTTCAGAAG
ATCAGCGAGTGTGAGGCTGTGCACCCTGTGAAAAACTGGATGGACATGAAGCGGCGTGTGGGGCCCTACC
GGAGATGTTACTTCTTCTCCCACTGCTCCACCCCCGGGGAACCCCTGGTGGTTCTGCATGTGGCTCTGAC
TGGTGACATTTCCAACAACATCCAGGGCATTGTGAAGGAGTGCCCTCCGACTGAAACCGAGGAGAGGAAC
AGGATCGCCGCTGCCATCTTCTACTCCATCAGCCTGACCCAGCAGGGCCTGCAGGGGGTGGAGCTCGGGA
CCTTCCTCATAAAGAGAGTGGTCAAGGAGCTGCAGAAGGAATTTCCTCAGCTGGGGGCCTTTTCAAGCCT
GTCGCCTATCCCTGGATTCACCAAGTGGCTGCTGGGCCTCCTGAATGTGCAGGGGAAGGAGCATGGGAGG
AACGAACTATTCACAGACTCAGAGTGCCAAGAAATCTCAGCGGTTACCGGCAACCCTGTTCACGAGAGCC
TCAAGGGGTTCCTGAGCAGTGGTGAGTGGGTGAAGTCAGAGAAGCTGACGCAGGCGCTGCAGGGGCCACT
AATGAGGCTGTGTGCCTGGTACCTGTACGGTGAGAAGCACCGAGGCTACGCCCTCAACCCAGTGGCCAAC
TTCCATCTGCAGAATGGGGCTGTGATGTGGCGTATCAACTGGATGGCTGACAGCAGCCTCAAAGGCCTCA
CCAGCTCATGCGGCCTCATGGTCAACTACCGCTACTACCTGGAGGAGACAGGCCCCAACAGTATCTCCTA
CCTGGGCTCCAAGAACATCAAAGCTTCCGAGCAGATCCTCAGCCTGGTAGCCCAGTTCCAGAACAACAGC
AAACTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207886 representing NM_019966
Red=Cloning site Green=Tags(s)

MRGLGPGLRARRLLPLRSPPRPPGPRGRRLCGGLAASAMDELLRRAVPPTPAYELREKTPAPAEGQCADF
VSFYGGLAEASQRAELLGRLAQGFGVDHGQVAEQSAGVLQLRQQAREAAVLLQAEDRLRYALVPRYRGLF
HHISKLDGGVRFLVQLRADLLEAQALKLVEGPHVREMNGVLKSMLSEWFSSGFLNLERVTWHSPCEVLQK
ISECEAVHPVKNWMDMKRRVGPYRRCYFFSHCSTPGEPLVVLHVALTGDISNNIQGIVKECPPTETEERN
RIAAAIFYSISLTQQGLQGVELGTFLIKRVVKELQKEFPQLGAFSSLSPIPGFTKWLLGLLNVQGKEHGR
NELFTDSECQEISAVTGNPVHESLKGFLSSGEWVKSEKLTQALQGPLMRLCAWYLYGEKHRGYALNPVAN
FHLQNGAVMWRINWMADSSLKGLTSSCGLMVNYRYYLEETGPNSISYLGSKNIKASEQILSLVAQFQNNS
KL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019966
ORF Size 1476 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019966.3
RefSeq Size 2116 bp
RefSeq ORF 1479 bp
Locus ID 56690
UniProt ID Q99J39
Cytogenetics 8 E1
MW 55.2 kDa
Summary Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Plays a role in the metabolic balance between glucose and lipid oxidation in muscle independent of alterations in insulin signaling. Plays a role in controlling the extent of ischemic injury by promoting glucose oxidation.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_019966" in other vectors (4)
SKU Description Size Price
MC200035 Mlycd (untagged) - Mouse malonyl-CoA decarboxylase (Mlycd), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$457.00
MG207886 Mlycd (tGFP-tagged) - Mouse malonyl-CoA decarboxylase (Mlycd) 10 ug
$489.00 $657.00
MR207886L3 Lenti ORF clone of Mlycd (Myc-DDK-tagged) - Mouse malonyl-CoA decarboxylase (Mlycd), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR207886L4 Lenti ORF clone of Mlycd (mGFP-tagged) - Mouse malonyl-CoA decarboxylase (Mlycd), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR207886

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207886
Circular map for MR207886

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products