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Sfrs4 (BC019437) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207825
Sfrs4 (Myc-DDK-tagged) - Mouse splicing factor, arginine/serine-rich 4 (SRp75) (cDNA clone MGC:30385 IMAGE:4018178)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sfrs4
Synonyms 5730499P16Rik; AW550192; MNCb-2616; Sfrs4; SRp75
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207825 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCGGGTGTACATCGGCCGCCTGAGCTACCAGGCCCGGGAGCGCGATGTGGAGCGCTTCTTTAAGG
GCTACGGGAAGATCCTGGAGGTGGATCTGAAGAACGGGTATGGCTTTGTGGAGTTTGATGATCTGCGTGA
TGCAGACGATGCTGTTTATGAACTGAACGGCAAAGACCTGTGTGGCGAGCGAGTCATTGTTGAGCACGCT
CGAGGCCCGCGGCGGGATGGCAGCTACGGCTCGGGACGCAGTGGATATGGTTATCGAAGAAGTGGCCGAG
ATAAATACGGTCCCCCTACTCGCACAGAATACAGACTTATTGTGGAGAATTTGTCAAGTCGATGCAGCTG
GCAGGACCTAAAGGATTACATGCGTCAGGCAGGAGAAGTGACGTATGCAGATGCTCACAAGGGACGCAAA
AACGAAGGAGTGATTGAATTTGTGTCTTACTCTGATATGAAAAGAGCTTTGGAAAAGCTGGACGGAACTG
AAGTCAACGGCAGAAAAATCAGATTAGTTGAAGACAAGCCAGGTTCTAGGCGGCGCCGGTCTTACTCCAG
AAGCCGGAGTCACTCAAGGTCTCGCTCTCGAAGCAGACATTCCCGGAAGAGCAGAAGCCGGAGTGGTAGC
AGTAAAAGCAGCCATTCGAAGAGCCGGTCTCGATCCAGGTCAGGTTCCCACTCCCGCAGCAAGAGCCGCA
GTCGCAGTCAGAGCCGCAGCCGCAGTAAGAAGGAGAAAAGCCGGAGCCCCAGCAAGGACAACAAGAGCCG
CAGTCGCAGCCGCAGCCCTGACAAGAGCCGCAGTAAGAGTAAAGACCACGCCGAGGACAAGCTCCAAAAC
AACGACAGCGCCGGCAAAGCCAAGAGCCACAGCCCCAGCCGCCATGACAGTAAAAGTCGGAGCAGGAGCC
AGGAGAGGAGAGCAGAGGAGGAGAGGCGAAGGAGCGTGAGCAGGGCTCGCAGCCAGGAGAAGAGCCGCAG
CCAGGAGAAGAGCCTTCTCAAGAGCCGCAGCCGCAGCCGGAGCCGCAGCCGCAGCCGCAGCAAGGACAAG
CGGAAGGGAAGGAAGAGAAGCAGGGACGAAAGTCGAAGCCGCAGCCGCAGCAAGAGCGAGCGTAGCCGAA
AACACAGCAGCAAGCGGGACAGCAAAGTCAGTAGCAGCAGCAGCAGCAGCAAGAAGAAGAAGGACACCGA
CCACTCCCGGTCACCGTCCCGCTCGGTGTCAAAGGAGCGAGAACATGCCAAGGCAGAGTCTGGCCAGAGG
GGAAGCCGGGCAGAGGGTGAGAGCGAGGCCCCCAATCCAGAGCCCCGGGCCAGGTCAAGATCCACCTCCA
AATCCAAACCCAATGTCCCGGCAGAGTCGCGCTCCAGGTCAAAATCGGCTTCAAAAACACGCTCTCGGTC
CAAGTCTCCATCCAGGTCTGCTTCCAGGTCGCCCTCCCGGTCTAGATCCAGGTCCCACTCAAGGTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207825 protein sequence
Red=Cloning site Green=Tags(s)

MPRVYIGRLSYQARERDVERFFKGYGKILEVDLKNGYGFVEFDDLRDADDAVYELNGKDLCGERVIVEHA
RGPRRDGSYGSGRSGYGYRRSGRDKYGPPTRTEYRLIVENLSSRCSWQDLKDYMRQAGEVTYADAHKGRK
NEGVIEFVSYSDMKRALEKLDGTEVNGRKIRLVEDKPGSRRRRSYSRSRSHSRSRSRSRHSRKSRSRSGS
SKSSHSKSRSRSRSGSHSRSKSRSRSQSRSRSKKEKSRSPSKDNKSRSRSRSPDKSRSKSKDHAEDKLQN
NDSAGKAKSHSPSRHDSKSRSRSQERRAEEERRRSVSRARSQEKSRSQEKSLLKSRSRSRSRSRSRSKDK
RKGRKRSRDESRSRSRSKSERSRKHSSKRDSKVSSSSSSSKKKKDTDHSRSPSRSVSKEREHAKAESGQR
GSRAEGESEAPNPEPRARSRSTSKSKPNVPAESRSRSKSASKTRSRSKSPSRSASRSPSRSRSRSHSRS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC019437
ORF Size 1467 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC019437, AAH19437
RefSeq Size 2204 bp
RefSeq ORF 1469 bp
Locus ID 57317
Cytogenetics 4 D2.3
MW 56 kDa
Summary The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. provided by RefSeq, Sep 2010
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"BC019437" in other vectors (4)
SKU Description Size Price
MC217805 Sfrs4 (untagged) - Mouse splicing factor, arginine/serine-rich 4 (SRp75) (cDNA clone MGC:30385 IMAGE:4018178), (10ug) 10 ug
$503.00
MG207825 Sfrs4 (tGFP-tagged) - Mouse splicing factor, arginine/serine-rich 4 (SRp75) (cDNA clone MGC:30385 IMAGE:4018178) 10 ug
$657.00
MR207825L3 Lenti ORF clone of Sfrs4 (Myc-DDK-tagged) - Mouse splicing factor, arginine/serine-rich 4 (SRp75) (cDNA clone MGC:30385 IMAGE:4018178) 10 ug
$757.00
MR207825L4 Lenti ORF clone of Sfrs4 (mGFP-tagged) - Mouse splicing factor, arginine/serine-rich 4 (SRp75) (cDNA clone MGC:30385 IMAGE:4018178) 10 ug
$757.00

Plasmid Map for MR207825

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207825
Circular map for MR207825

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