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Dusp10 (NM_022019) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207734
Dusp10 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 10 (Dusp10)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dusp10
Synonyms 2610306G15Rik; AI158871; MKP-5; MKP5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207734 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCCATCTCCTTTAGACGACAGGGTAGTAGTGGCACTATCTAGGCCTGTCCGACCTCAGGATCTCA
ACCTTTGTTTAGACTCAAGCTACCTTGGCTCTGCCAGCCCAGGTAGTGGTAGCCACGCTCCTGTCCTTGC
CACCGCCGTTGTGACCCTCAAGGCTGCGAATCTGACGTATATGCCCTCATCCAGCGGCTCTGCCCGCTCC
CTGAATTGCGGATGCAGCAGCACTAGCTGCTGCACTGTGGCAACCTACGACAAGGATCATCAGGCCCAGA
CTCAAGCCATTGCTGCAGGCACTGCCACCACCGCCATAGGAACCTCGACCACCTGCCCTGCTAACCAGAT
GGTCAACAACAATGAAAACACAGGCTCTGTGTTAAGTCCATCGGGTGGGGTGGGCAGCCCTGTGTCAGGG
ACTCCCAAGCAGCTAGCCAGCATCAAGATCATCTACCCCAATGACCTGGCAAAGAAGATGACCAAATGCA
GCAAGAGTCACCTGCCCAGCCAGGGTCCCGTCATCATCGATTGCAGGCCTTTCATGGAGTACAACAAGAG
TCACATCCAAGGAGCTGTCCACATTAACTGTGCCGATAAGATCAGCCGGCGAAGGCTGCAGCAGGGCAAG
ATCACTGTCTTAGACTTGATTTCCTGTAGGGAAGGCAAAGACTCTTTCAAGAGGATCTTTTCCAAAGAAA
TTATAGTTTATGATGAGAATACCAATGAGCCAAGCCGTGTGACACCATCCCAGCCACTTCACATAGTCCT
TGAGTCCCTGAAGAGAGAAGGCAAAGAACCCCTGGTATTGAAAGGGGGACTCAGTAGTTTTAAACAGAAC
CATGGAAACCTCTGTGACAACTCCCTCCAGCTCCAAGAGTGCCGGGAGGTGGGGGGTGGTGCATCTGCGG
CCTCGAGCATGCTACCTCAGTCTGTCCCCACCACCCCTGACATCGAGAACGCAGAGCTAACGCCCATCCT
GCCCTTCCTGTTCCTCGGCAATGAGCAGGATGCTCAGGACCTAGACACCATGCAGAGGCTCAACATCGGC
TATGTCATCAACGTCACCACGCACCTTCCTCTGTACCATTATGAGAAAGGCCTCTTCAACTACAAGAGGC
TGCCAGCCACAGACAGCAACAAACAGAACCTGCGGCAGTACTTTGAAGAGGCCTTCGAGTTCATCGAGGA
AGCTCACCAGTGTGGGAAGGGCCTTCTCATCCACTGCCAGGCCGGCGTGTCCCGATCCGCCACCATCGTC
ATCGCCTACTTGATGAAGCACACACGGATGACCATGACTGACGCTTACAAATTCGTCAAAGGCAAACGAC
CAATTATTTCCCCGAACCTCAACTTCATGGGGCAGTTGCTGGAATTTGAGGAAGACCTAAACAACGGCGT
GACGCCAAGAATCCTTACACCAAAGCTCATGGGCATGGAGACAGTTGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207734 protein sequence
Red=Cloning site Green=Tags(s)

MPPSPLDDRVVVALSRPVRPQDLNLCLDSSYLGSASPGSGSHAPVLATAVVTLKAANLTYMPSSSGSARS
LNCGCSSTSCCTVATYDKDHQAQTQAIAAGTATTAIGTSTTCPANQMVNNNENTGSVLSPSGGVGSPVSG
TPKQLASIKIIYPNDLAKKMTKCSKSHLPSQGPVIIDCRPFMEYNKSHIQGAVHINCADKISRRRLQQGK
ITVLDLISCREGKDSFKRIFSKEIIVYDENTNEPSRVTPSQPLHIVLESLKREGKEPLVLKGGLSSFKQN
HGNLCDNSLQLQECREVGGGASAASSMLPQSVPTTPDIENAELTPILPFLFLGNEQDAQDLDTMQRLNIG
YVINVTTHLPLYHYEKGLFNYKRLPATDSNKQNLRQYFEEAFEFIEEAHQCGKGLLIHCQAGVSRSATIV
IAYLMKHTRMTMTDAYKFVKGKRPIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGMETVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022019
ORF Size 1449 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022019.1, NM_022019.2, NM_022019.3, NM_022019.4, NM_022019.5, NP_071302.2
RefSeq Size 2712 bp
RefSeq ORF 1452 bp
Locus ID 63953
UniProt ID Q9ESS0
Cytogenetics 1 H5
MW 52.5 kDa
Summary Protein phosphatase involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily. It preferably dephosphorylates p38.UniProtKB/Swiss-Prot Function
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"NM_022019" in other vectors (4)
SKU Description Size Price
MC203171 Dusp10 (untagged) - Mouse dual specificity phosphatase 10 (Dusp10), (10ug) 10 ug
$457.00
MG207734 Dusp10 (tGFP-tagged) - Mouse dual specificity phosphatase 10 (Dusp10) 10 ug
$657.00
MR207734L3 Lenti ORF clone of Dusp10 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 10 (Dusp10) 10 ug
$757.00
MR207734L4 Lenti ORF clone of Dusp10 (mGFP-tagged) - Mouse dual specificity phosphatase 10 (Dusp10) 10 ug
$757.00

Plasmid Map for MR207734

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207734
Circular map for MR207734

file-alt Citations

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