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Eif2s3y (NM_012011) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207547
Eif2s3y (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked (Eif2s3y)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Eif2s3y or NM_012011"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eif2s3y
Synonyms Eif-2gy; Spy; Tfy
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207547 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCGGAGAAGCCGGTGTCACTCTCGGGCAGCCGCATCTTTCTCGTCAGGATCTTGCCACTTTGG
ATGTTACCAAGTTGACTCCGCTTTCACGTGAAATTATCAGCAGACAAGCCACAATTAATATAGGCACAAT
TGGTCATGTTGCTCATGGAAAATCTACAGTTGTAAAAGCCATTTCTGGTGTTCACACTGTCCGATTCAAA
AATGAACTAGAAAGGAATATTACCATAAAACTTGGATATGCTAATGCCAAAATTTATAAGCTTGATGACT
CAAGTTGTCCTCGACCAGAATGTTACAGATCTTGTGGAAGTAGTACACCTGATGAGTTTCCTTCAGATAT
TCCAGGGACCAAAGGAAACTTCAGACTAGTCAGACATGTTTCCTTTGTTGATTGTCCTGGTCATGATATT
TTGATGGCAACTATGCTGAATGGGGCAGCAGTGATGGATGCAGCTCTTCTGTTGATAGCTGGTAATGAAT
CTTGTCCTCAACCTCAGACTTCTGAACACCTGGCTGCCATTGAAATTATGAAGCTAAAACATATTTTGAT
TCTGCAAAATAAAATTGATTTGGTGAAAGAAAGCCAGGCTAAAGAACAGTATGAACAGATACTTGCATTT
GTACAGGGTACAGTAGCCGAAGGAGCTCCTATTATTCCAATTTCTGCTCAGTTAAAATACAATATTGAAG
TTGTATGTGAGTATATAGTAAAGAAAATTCCAGTACCTCTAAGAGACTTTACTTCAGAACCCCGACTTAT
TGTTATTCGGTCTTTTGATGTTAACAAACCTGGCTGTGAAGTTGATGACCTTAAAGGGGGTGTAGCTGGT
GGTAGTATTTTAAAAGGCGTATTAAAGGTGGGACAAGAGATAGAAGTGAGACCTGGTATTGTTTCTAAAG
ACGGAGAAGGGAAGCTTATGTGTAAACCAATCTTTTCCAAGATTGTATCCCTTTTTGCAGAACACAATGA
TCTTCAGTATGCTGCTCCAGGTGGTCTTATTGGAGTTGGAACAAAAATTGACCCAACGTTATGCCGAGCA
GATAGAATGGTTGGGCAGGTCCTTGGTGCTGTTGGAGCATTACCTGAGATATTCACAGAGTTAGAAATTT
CCTACTTCCTACTGAGACGGCTCCTAGGTGTACGTACAGAAGGAGACAAGAAAGCAGCAAAAGTTCAAAA
GCTATCCAAGAATGAAGTACTCATGGTGAACATAGGGTCCTTGTCTACAGGAGGCAGAGTTAGTGCAGTC
AAGGCAGATTTGGGTAAAATTGTTCTAACCAATCCAGTATGCACAGAAGTAGGAGAAAAAATTGCTCTAA
GCCGACGAGTTGAGAAACACTGGCGTTTAATTGGTTGGGGCCAGATAAGAAGAGGCGTGACTATCAAGCC
AACAATAGATGATGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207547 protein sequence
Red=Cloning site Green=Tags(s)

MAGGEAGVTLGQPHLSRQDLATLDVTKLTPLSREIISRQATINIGTIGHVAHGKSTVVKAISGVHTVRFK
NELERNITIKLGYANAKIYKLDDSSCPRPECYRSCGSSTPDEFPSDIPGTKGNFRLVRHVSFVDCPGHDI
LMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHILILQNKIDLVKESQAKEQYEQILAF
VQGTVAEGAPIIPISAQLKYNIEVVCEYIVKKIPVPLRDFTSEPRLIVIRSFDVNKPGCEVDDLKGGVAG
GSILKGVLKVGQEIEVRPGIVSKDGEGKLMCKPIFSKIVSLFAEHNDLQYAAPGGLIGVGTKIDPTLCRA
DRMVGQVLGAVGALPEIFTELEISYFLLRRLLGVRTEGDKKAAKVQKLSKNEVLMVNIGSLSTGGRVSAV
KADLGKIVLTNPVCTEVGEKIALSRRVEKHWRLIGWGQIRRGVTIKPTIDDE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012011
ORF Size 1416 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012011.2
RefSeq Size 1801 bp
RefSeq ORF 1419 bp
Locus ID 26908
UniProt ID Q9Z0N2
Cytogenetics Ypter
MW 51.1 kDa
Summary As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome X, may contribute to spermatogenesis up to the round spermatid stage (PubMed:26823431).UniProtKB/Swiss-Prot Function
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"NM_012011" in other vectors (4)
SKU Description Size Price
MC204459 Eif2s3y (untagged) - Mouse eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked (Eif2s3y), (10ug) 10 ug
$457.00
MG207547 Eif2s3y (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked (Eif2s3y) 10 ug
$657.00
MR207547L3 Lenti ORF clone of Eif2s3y (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked (Eif2s3y) 10 ug
$757.00
MR207547L4 Lenti ORF clone of Eif2s3y (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked (Eif2s3y) 10 ug
$757.00

Plasmid Map for MR207547

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR207547
Circular map for MR207547

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