Rtn2 (NM_013648) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207524
Rtn2 (Myc-DDK-tagged) - Mouse reticulon 2 (Z-band associated protein) (Rtn2), transcript variant B
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rtn2
Synonyms MMS10-P; Ms10p; Nspl1; NSPLI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207524 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCAGGTCCTGCCGGTCTTCGCCCACTGCAAAGAAGCTCCATCTACAGCCTCTTCTACCCCCGATT
CCACGGAAGGAGGGAATGACGACTCAGATTTTCGGGAGCTGCACACAGCCCGGGAATTCTCAGAGGATGA
AGAGGAGGAGACCACATCGCAGGACTGGGGCACCCCCAGGGAGCTGACCTTTTCCTACATTGCCTTCGAC
GGCGTGGTGGGCTCTGGGGGCCGTAGGGACTCAGTTGTTCGTCGTCCCCGGCCCCAGGGACGTTCAGTCT
CAGAACCACGGGACCCACCCCAACAGTCCGGCCTGGGTGACAGCTTGGAAAGCATCCCTAGCCTGAGCCA
GTCCCCAGAGCCAGGGCGCCGCGGTGACCCTGACCCCGTGCCTCCAGCTGAGCGCCCGCTGGAGGAACTG
AGGCTGCGGCTGGACCAGCTTGGCTGGGTTGTCCGGAGTGCGGGATCTGGGGAGGATTCGGCCACCAGCA
GCTCCACCCCTCTGGAAAATGAGGAGCCTGATGGATTGGAGGCCAGCGAGGCTGGAGAAGAGACGAATCT
GGAACTCCGACTTGCTCAGTCTCTACATTTGCAACTTGAGGTCCTGACTCCTCAACTTAGCCCTAGCTCC
GGGACCCCCCAGGCGCATACTCCATCCCCACAAAGATCTCAAGATTCGAACTCCGGTCCTGATGATGAGC
CTCTGCTGAATGTGGTAGAAGAGCATTGGCGGCTGCTGGAGCAGGAGCCAATCACGGCCCAGTGCCTCGA
TAGCACAGACCAATCAGAATTCATGTTGGAGCCGCTCCTTCTAGTGGCAGACCTGTTGTACTGGAAGGAC
ACCAGGACGTCGGGAGCGGTCTTCACGGGCCTCATGGCCTCTCTCCTCTGCCTCCTGCACTTTAGCATCG
TGTCCGTGGCTGCGCACCTGGCCCTGTTGGGTCTCTGCGCCACCATCTCTCTCAGGGTTTACCGCAAAGT
GCTGCAGGCCGTGCACCGGGGCGACGGCACCAACCCTTTCCAGGCCTACCTGGACATGGACCTGACCCTG
ACTCGGGAGCAGACAGAACGTTTGTCCCAGCAGATCGCCTCCCACGTGGTCTCCACAGCCACACAGCTAC
GGCATTTCTTCCTAGTGGAAGACCTGGTGGATTCTCTCAAGCTGGCTCTTCTCTTCTACATCTTGACCTT
TGTGGGAGCCATCTTCAACGGCTTGACTCTTGTCATCCTAGGAGTGGTTGCTTTGTTTACCGTCCCCCTT
CTGTACAGACAACATCAGGCCCAGATTGACCAGTATGTGGGGTTGGTGACCAATCAGTTGAGCCACATCA
AAGCTAAGATCCGAGCTAAGATCCCGGGGACCGGAACCCTTGCCCCAACAGCATCAGTCTCCGGATCCAA
AGCCAAAGCCGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207524 protein sequence
Red=Cloning site Green=Tags(s)

MGQVLPVFAHCKEAPSTASSTPDSTEGGNDDSDFRELHTAREFSEDEEEETTSQDWGTPRELTFSYIAFD
GVVGSGGRRDSVVRRPRPQGRSVSEPRDPPQQSGLGDSLESIPSLSQSPEPGRRGDPDPVPPAERPLEEL
RLRLDQLGWVVRSAGSGEDSATSSSTPLENEEPDGLEASEAGEETNLELRLAQSLHLQLEVLTPQLSPSS
GTPQAHTPSPQRSQDSNSGPDDEPLLNVVEEHWRLLEQEPITAQCLDSTDQSEFMLEPLLLVADLLYWKD
TRTSGAVFTGLMASLLCLLHFSIVSVAAHLALLGLCATISLRVYRKVLQAVHRGDGTNPFQAYLDMDLTL
TREQTERLSQQIASHVVSTATQLRHFFLVEDLVDSLKLALLFYILTFVGAIFNGLTLVILGVVALFTVPL
LYRQHQAQIDQYVGLVTNQLSHIKAKIRAKIPGTGTLAPTASVSGSKAKAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013648
ORF Size 1413 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013648.6
RefSeq Size 1994 bp
RefSeq ORF 1416 bp
Locus ID 20167
UniProt ID O70622
Cytogenetics 7 9.54 cM
MW 51.3 kDa
Summary Inhibits amyloid precursor protein processing, probably by blocking BACE1 activity (By similarity). Enhances trafficking of the glutamate transporter SLC1A1/EAAC1 from the endoplasmic reticulum to the cell surface (By similarity). Plays a role in the translocation of SLC2A4/GLUT4 from intracellular membranes to the cell membrane which facilitates the uptake of glucose into the cell (PubMed:19720795).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013648" in other vectors (4)
SKU Description Size Price
MC205806 Rtn2 (untagged) - Mouse reticulon 2 (Z-band associated protein) (Rtn2), transcript variant B, (10ug) 10 ug
$457.00
MG207524 Rtn2 (tGFP-tagged) - Mouse reticulon 2 (Z-band associated protein) (Rtn2), transcript variant B 10 ug
$657.00
MR207524L3 Lenti ORF clone of Rtn2 (Myc-DDK-tagged) - Mouse reticulon 2 (Z-band associated protein) (Rtn2), transcript variant B 10 ug
$757.00
MR207524L4 Lenti ORF clone of Rtn2 (mGFP-tagged) - Mouse reticulon 2 (Z-band associated protein) (Rtn2), transcript variant B 10 ug
$757.00

Plasmid Map for MR207524

Download sequence for

Cloning scheme for MR207524

Circular map for MR207524

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