Steap4 (NM_054098) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207521
Steap4 (Myc-DDK-tagged) - Mouse STEAP family member 4 (Steap4)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Steap4
Synonyms 1110021O17Rik; AI481214; Tiarp; Tnfaip9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207521 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAAGCACATGCAGATGAGTTTCCTCTGACTACTGATTCCTCAGAAAAGCAAGGGGTTGTCTGCA
TTTTCGGAACAGGGGATTTCGGGAAGTCACTGGGATTGAAAATGCTTCAGTGTGGCTATTCTATCGTTTT
TGGGAGTCGAAACCCCCAGGTGTCCAGCCTTCTGCCCAGGGGGGCAGAGGTCCTGAGCTATTCGGAAGCA
GCATCCAAGTCTGACATCATAATCCTAGCCATGCACAGAGAGCACTATGATTCCCTCACGGAACTAGTTG
ATTATCTCAAAGGAAAAGTATTGGTAGACGTCAGTAACAACCGCAAAATCAATCAGTATCCAGAGTCAAA
TGCGGAATACCTTGCTCAGCTGGAGCCAGGAGCCCACGTGGTCAAAGCATTTAACACCATCTCAGCCTGG
GCTCTCCAGTCAGGAACACTAGATGCAAGCCGGCAGGTGTTTGTCTGTGGAAATGACAGCAAGGCCAAAC
AAAGAGTAATGGACATTGCTCGTACTCTTGGGCTCACCCCACTGGACCAAGGATCTCTCATGGCAGCCAG
TGAAATTGAAAACTACCCTCTGCAACTATTTCCAATGTGGAGGTTTCCTTTCTATTTGTCCTCTGTGCTG
TGCGTCTTCTTCTTTGTGTACTGTGCAATACGAGAGGTGATATACCCCTACGTGAATGGGAAAACAGATG
CTACGTACCGCCTGGCCATTTCCATCCCGAATCGTGTCTTTCCTATAACAGCACTGATCCTGCTTGCCTT
GGTGTACCTCCCTGGTATTCTCGCTGCCATTCTGCAGCTCTATCGAGGCACAAAGTACCGCCGATTCCCA
AACTGGCTTGACCATTGGATGCTTTGCAGAAAGCAACTGGGCTTGGTAGCTCTGGGATTTGCCTTCCTTC
ATGTCATCTACACACTTGTGATTCCTATCCGTTACTATGTTCGATGGAGGCTGAGAAATGCAACCATTAC
TCAGGCCCTAACTAATAAAGACAGCCCATTTATTACCTCTTATGCCTGGATTAATGATTCCTACTTGGCG
CTGGGAATCCTGGGATTTTTTCTGTTCCTTCTGTTGGGAATCACTTCCTTGCCATCAGTAAGCAACATGG
TCAACTGGAGAGAGTTCCGGTTTGTTCAGTCCAAACTGGGTTATCTGACCCTGGTCTTGTGCACAGCCCA
CACTTTGGTGTATGGCGGAAAGAGATTCCTCAGCCCTTCGATTCTAAGGTGGAGCCTCCCTTCGGCTTAT
ATTTTGGCACTCGTCATCCCCTGCGCAGTGCTGGTGCTGAAGTGCATCCTCATCATGCCATGCATAGACA
AGACCCTCACTCGCATCCGCCAGGGCTGGGAAAGAAACTCAAAGTACACACAATCAGCACTGAACGGAAA
ATCGGATATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207521 protein sequence
Red=Cloning site Green=Tags(s)

MEKAHADEFPLTTDSSEKQGVVCIFGTGDFGKSLGLKMLQCGYSIVFGSRNPQVSSLLPRGAEVLSYSEA
ASKSDIIILAMHREHYDSLTELVDYLKGKVLVDVSNNRKINQYPESNAEYLAQLEPGAHVVKAFNTISAW
ALQSGTLDASRQVFVCGNDSKAKQRVMDIARTLGLTPLDQGSLMAASEIENYPLQLFPMWRFPFYLSSVL
CVFFFVYCAIREVIYPYVNGKTDATYRLAISIPNRVFPITALILLALVYLPGILAAILQLYRGTKYRRFP
NWLDHWMLCRKQLGLVALGFAFLHVIYTLVIPIRYYVRWRLRNATITQALTNKDSPFITSYAWINDSYLA
LGILGFFLFLLLGITSLPSVSNMVNWREFRFVQSKLGYLTLVLCTAHTLVYGGKRFLSPSILRWSLPSAY
ILALVIPCAVLVLKCILIMPCIDKTLTRIRQGWERNSKYTQSALNGKSDI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_054098
ORF Size 1410 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_054098.2
RefSeq Size 3140 bp
RefSeq ORF 1413 bp
Locus ID 117167
UniProt ID Q923B6
Cytogenetics 5 A1
MW 53 kDa
Summary Integral membrane protein that functions as NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane (PubMed:16609065). Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate (By similarity). Can also reduce Cu(2+) to Cu(1+) (PubMed:16609065). Plays a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses (PubMed:17482547). Associated with obesity and insulin-resistance (By similarity). Involved in inflammatory arthritis, through the regulation of inflammatory cytokines (PubMed:19660107). Inhibits anchorage-independent cell proliferation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_054098" in other vectors (3)
SKU Description Size Price
MG207521 Steap4 (tGFP-tagged) - Mouse STEAP family member 4 (Steap4) 10 ug
$657.00
MR207521L3 Lenti ORF clone of Steap4 (Myc-DDK-tagged) - Mouse STEAP family member 4 (Steap4) 10 ug
$757.00
MR207521L4 Lenti ORF clone of Steap4 (mGFP-tagged) - Mouse STEAP family member 4 (Steap4) 10 ug
$757.00

Plasmid Map for MR207521

Download sequence for

Cloning scheme for MR207521

Circular map for MR207521

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