Ifit1 (NM_008331) Mouse Tagged ORF Clone

SKU MR207393

Ifit1 (Myc-DDK-tagged) - Mouse interferon-induced protein with tetratricopeptide repeats 1 (Ifit1)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ifit1
• Synonyms: Ifi56; ISG56; P56
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207393 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGAGAGAATGCTGATGGTGACCAGGTCATGGAGAATCTGCTTCAGCTGAGATGTCACTTCACATGGA; AGCTGCTATTTGAAAATAATGACATACCTGATTTGGAAGTGAGAATCTCAGAGCAGGTCCAGTTCCTTGA; CATCAAGAACCCATTGGGGATGCACAACCTCCTGGCCTACGTGAGGCACCTGAAAGGCCAGCAGGACGAA; GCCCTGCAGAGCTTGAAAGAAGCTGAAGCCTTGATCCAGAGCGAGCAGCTGAGCAAGAGAAGCCTGGCGA; CCTGGGGCAACTGTGCCTGGCTGCATTACCACAGGGGCAGCTTGGCAGAAGCCCAGATCTACCTGGACAA; GGTGGAGAAGGTGTGCAAGGAATTTTCAAGTCCCTTCCGCTACAGGCTGGAGTGTGCTGAGATGGACTGT; GAGGAAGGCTGGGCCTTGCTGAAGTGTGGAGGAGGAAATTATAAACAAGCCATGGCCTGCTTTGCGAAGG; CTCTGAAAGTGGAGCCAGAAAACCCTGAGTACAACACTGGCTATGCAGTCGTAGCCTATCGCCAAGATTT; AGATGACAACTTTATTTCTCTAGAACCTTTGAGGAAGGCTGTCCGGTTAAATCCAGAAGATCCATACCTT; AAAGTTCTCCTTGCCCTAAAGCTTCAGGATTTAGGAGAACATGTTGAAGCAGAAGCACACATTGAAGAAG; CCCTCAGCAGCACATCTTGCCAAAGCTATGTCATTCGCTATGCAGCCAAATATTTCCGTAGGAAACATCG; CGTAGACAAAGCTCTTCATCTTCTAAACAGGGCCTTGCAGGCATCACCTTCCTCTGGCTACTTACATTAT; CAAAAAGGGCTCTGCTACAAGCAACAAATCTCCCAACTGAGGACATCCCGAAACAGGCAGCCCAGAAGGC; AGGACAATGTGCAAGAATTGGCACAACAGGCCATTCATGAATTTCAAGAGACTTTGAAACTGAGGCCCAC; ATTTGAGATGGCCTATGTTTGCATGGCTGAAGTGCAGGCAGAAATTCACCAGTATGAAGAAGCAGAGAGA; AATTTCCAGAAGGCACTGAACAACAAGACCCTCGTGGCTCACATAGAGCAGGATATTCACCTCCGCTATG; GCCGTTTCCTACAGTTTCATAAGCAGTCAGAAGACAAGGCAATCACCCTCTACTTAAAAGGTCTAAAAGT; GGAAGAGAAGTCCTTTGCTTGGAGGAAACTACTGACTGCTTTGGAGAAAGTGGCTGAAAGACGTGTTTGT; CAGAATGTTCATCTTGTAGAGAGTACCAGCCTTCTTGGGCTAGTCTACAAACTGAAAGGACAAGAGAAAA; ATGCTCTGTTTTACTATGAGAAGGCACTGAGGCTCACTGGGGAAATGAACCCTGCATTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207393 protein sequence; Red=Cloning site Green=Tags(s); ; MGENADGDQVMENLLQLRCHFTWKLLFENNDIPDLEVRISEQVQFLDIKNPLGMHNLLAYVRHLKGQQDE; ALQSLKEAEALIQSEQLSKRSLATWGNCAWLHYHRGSLAEAQIYLDKVEKVCKEFSSPFRYRLECAEMDC; EEGWALLKCGGGNYKQAMACFAKALKVEPENPEYNTGYAVVAYRQDLDDNFISLEPLRKAVRLNPEDPYL; KVLLALKLQDLGEHVEAEAHIEEALSSTSCQSYVIRYAAKYFRRKHRVDKALHLLNRALQASPSSGYLHY; QKGLCYKQQISQLRTSRNRQPRRQDNVQELAQQAIHEFQETLKLRPTFEMAYVCMAEVQAEIHQYEEAER; NFQKALNNKTLVAHIEQDIHLRYGRFLQFHKQSEDKAITLYLKGLKVEEKSFAWRKLLTALEKVAERRVC; QNVHLVESTSLLGLVYKLKGQEKNALFYYEKALRLTGEMNPAF; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008331
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008331.3
• RefSeq Size: 2638 bp
• RefSeq ORF: 1392 bp
• Locus ID: 15957
• UniProt ID: Q64282
• Cytogenetics: 19 C1
• MW: 53.7 kDa
• Summary: Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism).[UniProtKB/Swiss-Prot Function]