Htra2 (NM_019752) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207308
Htra2 (Myc-DDK-tagged) - Mouse HtrA serine peptidase 2 (Htra2), nuclear gene encoding mitochondrial protein
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Htra2
Synonyms AI481710; mnd2; Omi; Prss25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207308 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGCTGAAAGCGGGGCGGGGTGCAAACTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATCT
TCTGGAGGAAGCCTCCCCTGTTAGCTCCTGACCTCCGGGCTCTGCTGACGTCAGGAACTCCTGACTCTCA
GATCTGGATGACTTATGGGACCCCCAGTCTCCCGGCCCAGGTGCCTGAGGGGTTCCTTGCATCACGGGCA
GATCTGACGTCTAGGACCCCGGATCTCTGGGCACGATTGAATGTGGGGACTTCAGGCTCCAGTGACCAGG
AGGCCCGCAGGAGCCCTGGAAGCCGTCGGCGCGAATGGCTGGCGGTGGCGGTGGGCGCAGGAGGTGCAGT
GGTGCTGCTGTTGTGGGGTTGGGGTCGGGGTCTTTCCACGGTGCTGGCTGCTGTTCCTGCTCCGCCACCC
ACTTCTCCCCGGAGCCAGTACAATTTCATCGCAGATGTGGTGGAGAAGACAGCCCCTGCTGTGGTCTATA
TCGAGATCCTAGACCGGCACCCTTTCTCCGGCCGTGAAGTCCCCATCTCAAACGGATCAGGATTCGTAGT
GGCTTCAGATGGGCTCATCGTTACCAACGCCCACGTGGTGGCTGATCGGCGCCGAGTACGAGTGAGGCTG
CCTAGCGGGGATACTTATGAGGCCATGGTCACAGCTGTGGATCCCGTAGCAGACATTGCCACACTGAGGA
TTCAAACCAAGGAGCCTCCTCCCACACTGCCCCTCGGCCGCTCTGCTGATGTCCGGCAAGGGGAGTTTGT
TGTTGCCATGGGAAGCCCCTTTGCACTGCAGAACACGATCACATCTGGTATTGTCAGCTCTGCTCAGCGC
CCAGCCAGGGACCTGGGACTCCCTCAAAACAACGTGGAATACATTCAGACCGATGCAGCTATTGATTTTG
GAAATTCTGGTGGTCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTGACAGC
TGGAATCTCCTTTGCCATCCCTTCTGATCGCCTTAGGGAGTTTCTGCATCGCGGGGAAAAGAAAAATTCC
TGGTTTGGAACCAGTGGGTCCCAGCGCCGCTACATTGGAGTGATGATGCTGACCCTGACTCCCAGCATCC
TTATTGAACTACAGCTCCGTGAGCCAAGCTTCCCTGATGTTCAGCATGGTGTCCTCATTCATAAAGTTAT
CCTGGGCTCCCCCGCACACAGGGCTGGTCTGCGGCCTGGTGATGTGATCTTGGCCATTGGGGAGAAATTG
GCACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCACAGCTGGCAGTGCGGATCCGGCGCG
GATCAGAGACACTGATCTTATATGTGACCCCCGAGGTCACAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207308 protein sequence
Red=Cloning site Green=Tags(s)

MAALKAGRGANWSLRAWRALGGIFWRKPPLLAPDLRALLTSGTPDSQIWMTYGTPSLPAQVPEGFLASRA
DLTSRTPDLWARLNVGTSGSSDQEARRSPGSRRREWLAVAVGAGGAVVLLLWGWGRGLSTVLAAVPAPPP
TSPRSQYNFIADVVEKTAPAVVYIEILDRHPFSGREVPISNGSGFVVASDGLIVTNAHVVADRRRVRVRL
PSGDTYEAMVTAVDPVADIATLRIQTKEPPPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQR
PARDLGLPQNNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNS
WFGTSGSQRRYIGVMMLTLTPSILIELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEKL
AQNAEDVYEAVRTQSQLAVRIRRGSETLILYVTPEVTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019752
ORF Size 1374 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019752.2, NP_062726.2
RefSeq Size 1739 bp
RefSeq ORF 1377 bp
Locus ID 64704
UniProt ID Q9JIY5
Cytogenetics 6 35.94 cM
MW 49.3 kDa
Summary Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019752" in other vectors (4)
SKU Description Size Price
MC202311 Htra2 (untagged) - Mouse HtrA serine peptidase 2 (Htra2), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$457.00
MG207308 Htra2 (tGFP-tagged) - Mouse HtrA serine peptidase 2 (Htra2) 10 ug
$657.00
MR207308L3 Lenti ORF clone of Htra2 (Myc-DDK-tagged) - Mouse HtrA serine peptidase 2 (Htra2), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR207308L4 Lenti ORF clone of Htra2 (mGFP-tagged) - Mouse HtrA serine peptidase 2 (Htra2), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR207308

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Cloning scheme for MR207308

Circular map for MR207308

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