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Pfkfb4 (BC057594) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207244
Pfkfb4 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (cDNA clone MGC:67170 IMAGE:6810667)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pfkfb4
Synonyms C230090D14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207244 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCTCCACGGGAACTGACCCAGAATCCCCTGAAGAAGATCTGGATGCCGTACAGCAATGGCAGGC
CCGCTCTGCACGCCAGCCAGCGTGGTGCCCGGGGCAAGACCTACATTTCTAAGAAGCTGACGCGGTACCT
CAACTGGATTGGCGTGCCTACTCGGGAATTCAACGTTGGTCAGTATCGCCGGGACATAGTCAAGACGTAC
AAATCTTTTGAGTTTTTCCTCCCAGACAATGAAGAAGGCCTGAAAATCAGGAAGCAGTGTGCCTTAGCAG
CCCTCAGTGATGTCCGGAAGTTCCTCAGTGAGGAGGGGGGACATGTGGCGGTTTTTGATGCAACCAATAC
GACCCGAGAGCGGAGAGCGATGATCTTTAACTTTGGAGAACAGAATGGCTACAAGACCTTCTTTGTTGAA
TCTATCTGTGTGGATCCTGAGGTTGTTGCTGCCAACATCGTGCAAGTGAAGCTGGGCAGCCCTGACTATG
TCAACCGGGACAGCGACGAAGCCACCGAAGACTTCATGAGGCGCATTGAATGCTATGAAAACTCATATGA
GTCGCTGGATGAAGACCTGGACAGGGATCTGTCCTACATCAAGATCATGGACGTGGGCCAGAGCTATGTG
GTGAACCGTGTTGCTGATCACATCCAGAGTCGCATCGTTTATTACCTCATGAACATCCATGTGACACCCC
GCTCCATCTACCTCTGCCGGCATGGGGAGAGCGAGCTAAACCTCAAGGGCCGGATTGGTGGGGATCCTGG
ACTGTCCCCCCGGGGCCGGGAGTTTTCCAAGCATCTGGCCCAGTTCATCAGTGACCAGAACATTAAGGAT
CTGAAGGTCTGGACGAGCCAGATGAAGAGGACGATCCAGACAGCCGAGGCGCTGAGCGTCCCTTATGAGC
AGTGGAAGGTCCTCAACGAGATCGATGCGGGCGTCTGTGAGGAAATGACCTACGAAGAAATCCAGGATCA
CTACCCGCTGGAGTTTGCCCTGCGGGATCAGGACAAGTACCGGTACCGGTACCCGAAGGGTGAGTCCTAT
GAAGACCTGGTGCAGCGGCTGGAGCCCGTCATCATGGAACTGGAGAGGCAGGAGAATGTGTTGGTCATTT
GCCACCAGGCTGTCATGCGCTGCCTCCTGGCCTACTTCCTTGACAAGGCAGCTGAAGAGCTGCCCTACCT
CAAATGCCCCTTGCACACAGTCCTGAAGCTCACACCCGTGGCTTACGGTTGTAAAGTGGAGTCCATATTC
CTGAACGTGGCAGCTGTGAACACTCACCGAGACAGACCTCAGAATGTAGACATATCCAGGCCTTCAGAGG
AAGCCCTTGTCACAGTCCCTGCTCACCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207244 protein sequence
Red=Cloning site Green=Tags(s)

MASPRELTQNPLKKIWMPYSNGRPALHASQRGARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDIVKTY
KSFEFFLPDNEEGLKIRKQCALAALSDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVE
SICVDPEVVAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYV
VNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFSKHLAQFISDQNIKD
LKVWTSQMKRTIQTAEALSVPYEQWKVLNEIDAGVCEEMTYEEIQDHYPLEFALRDQDKYRYRYPKGESY
EDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIF
LNVAAVNTHRDRPQNVDISRPSEEALVTVPAHQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC057594
ORF Size 1359 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC057594, AAH57594
RefSeq Size 3329 bp
RefSeq ORF 1361 bp
Locus ID 270198
Cytogenetics 9 F2
MW 52.4 kDa
Summary Synthesis and degradation of fructose 2,6-bisphosphate.UniProtKB/Swiss-Prot Function
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"BC057594" in other vectors (6)
SKU Description Size Price
MC218671 Pfkfb4 (untagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (cDNA clone MGC:67170 IMAGE:6810667), (10ug) 10 ug
$732.00
MG207244 Pfkfb4 (tGFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (cDNA clone MGC:67170 IMAGE:6810667) 10 ug
$886.00
MR207244L1 Lenti ORF clone of Pfkfb4 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (cDNA clone MGC:67170 IMAGE:6810667) 10 ug
$986.00
MR207244L2 Lenti ORF clone of Pfkfb4 (mGFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (cDNA clone MGC:67170 IMAGE:6810667) 10 ug
$986.00
MR207244L3 Lenti ORF clone of Pfkfb4 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (cDNA clone MGC:67170 IMAGE:6810667) 10 ug
$986.00
MR207244L4 Lenti ORF clone of Pfkfb4 (mGFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (cDNA clone MGC:67170 IMAGE:6810667) 10 ug
$986.00

Plasmid Map for MR207244

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207244
Circular map for MR207244

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